Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0329 and SMa5017 are separated by 55 nucleotides SMa5017 and SMa0333 overlap by 4 nucleotides SMa0333 and SMa0335 are separated by 180 nucleotides SMa0335 and SMa0337 overlap by 14 nucleotides
SMa0329: SMa0329 - short chain alcohol dehydrogenase-related dehydrogenase, at 182,145 to 182,906
SMa0329
SMa5017: SMa5017 - hypothetical protein, at 182,962 to 183,186
SMa5017
SMa0333: SMa0333 - hypothetical protein, at 183,183 to 183,395
SMa0333
SMa0335: SMa0335 - 3-ketoacyl-ACP reductase, at 183,576 to 184,352
SMa0335
SMa0337: SMa0337 - hypothetical protein, at 184,339 to 184,821
SMa0337
Position (kb) 183
184 Strain fitness (log2 ratio) -1
0
1
2 at 182.499 kb on + strand, within SMa0329 at 182.500 kb on - strand, within SMa0329 at 182.500 kb on - strand, within SMa0329 at 182.682 kb on - strand, within SMa0329 at 182.700 kb on - strand, within SMa0329 at 183.231 kb on - strand, within SMa0333 at 183.238 kb on + strand, within SMa0333 at 183.238 kb on + strand, within SMa0333 at 183.381 kb on - strand at 183.381 kb on - strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.628 kb on + strand at 183.629 kb on - strand at 183.629 kb on - strand at 183.868 kb on + strand, within SMa0335 at 183.868 kb on + strand, within SMa0335 at 184.242 kb on - strand, within SMa0335
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 182,499 + SMa0329 0.46 -0.3 182,500 - SMa0329 0.47 +0.6 182,500 - SMa0329 0.47 -0.4 182,682 - SMa0329 0.70 -0.2 182,700 - SMa0329 0.73 -0.6 183,231 - SMa0333 0.23 -0.9 183,238 + SMa0333 0.26 -0.4 183,238 + SMa0333 0.26 -1.2 183,381 - +2.3 183,381 - -0.8 183,628 + +0.8 183,628 + -0.1 183,628 + +0.8 183,628 + +0.8 183,628 + +0.0 183,629 - +0.3 183,629 - -0.1 183,868 + SMa0335 0.38 +0.2 183,868 + SMa0335 0.38 +0.2 184,242 - SMa0335 0.86 -0.4
Or see this region's nucleotide sequence