Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0235 and SMa0237 are separated by 8 nucleotides SMa0237 and SMa0241 overlap by 4 nucleotides
SMa0235: SMa0235 - dihydroxy-acid dehydratase, at 131,106 to 132,884
SMa0235
SMa0237: SMa0237 - dehydrogenase, at 132,893 to 133,774
SMa0237
SMa0241: SMa0241 - epimerase, at 133,771 to 134,754
SMa0241
Position (kb) 132
133
134 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 131.929 kb on + strand, within SMa0235 at 132.170 kb on - strand, within SMa0235 at 132.535 kb on + strand, within SMa0235 at 132.565 kb on + strand, within SMa0235 at 132.692 kb on + strand, within SMa0235 at 132.692 kb on + strand, within SMa0235 at 132.692 kb on + strand, within SMa0235 at 132.693 kb on - strand, within SMa0235 at 132.805 kb on + strand at 132.806 kb on - strand at 132.825 kb on - strand at 132.825 kb on - strand at 133.375 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.505 kb on - strand, within SMa0237 at 133.505 kb on - strand, within SMa0237 at 133.519 kb on + strand, within SMa0237 at 133.589 kb on - strand, within SMa0237 at 133.685 kb on - strand, within SMa0237 at 134.261 kb on + strand, within SMa0241 at 134.262 kb on - strand, within SMa0241 at 134.303 kb on + strand, within SMa0241 at 134.304 kb on - strand, within SMa0241 at 134.319 kb on + strand, within SMa0241 at 134.320 kb on - strand, within SMa0241 at 134.320 kb on - strand, within SMa0241 at 134.327 kb on + strand, within SMa0241 at 134.328 kb on - strand, within SMa0241 at 134.402 kb on + strand, within SMa0241 at 134.402 kb on + strand, within SMa0241 at 134.638 kb on + strand, within SMa0241 at 134.639 kb on - strand, within SMa0241 at 134.639 kb on - strand, within SMa0241 at 134.749 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 131,929 + SMa0235 0.46 +0.2 132,170 - SMa0235 0.60 -2.4 132,535 + SMa0235 0.80 +0.3 132,565 + SMa0235 0.82 +0.9 132,692 + SMa0235 0.89 +0.6 132,692 + SMa0235 0.89 +0.1 132,692 + SMa0235 0.89 +0.0 132,693 - SMa0235 0.89 -0.7 132,805 + -0.0 132,806 - +0.1 132,825 - -1.3 132,825 - +0.1 133,375 + SMa0237 0.55 +0.9 133,504 + SMa0237 0.69 -0.1 133,504 + SMa0237 0.69 -0.6 133,504 + SMa0237 0.69 -0.3 133,504 + SMa0237 0.69 -0.0 133,505 - SMa0237 0.69 -2.3 133,505 - SMa0237 0.69 -2.9 133,519 + SMa0237 0.71 +0.2 133,589 - SMa0237 0.79 -1.4 133,685 - SMa0237 0.90 -1.2 134,261 + SMa0241 0.50 -1.1 134,262 - SMa0241 0.50 -4.2 134,303 + SMa0241 0.54 -0.4 134,304 - SMa0241 0.54 -0.7 134,319 + SMa0241 0.56 +0.4 134,320 - SMa0241 0.56 +0.1 134,320 - SMa0241 0.56 +0.3 134,327 + SMa0241 0.57 -0.5 134,328 - SMa0241 0.57 -0.3 134,402 + SMa0241 0.64 -0.7 134,402 + SMa0241 0.64 +0.1 134,638 + SMa0241 0.88 -0.1 134,639 - SMa0241 0.88 +0.1 134,639 - SMa0241 0.88 +0.6 134,749 - +1.6
Or see this region's nucleotide sequence