Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0130 and SMa0132 overlap by 1 nucleotides SMa0132 and SMa0134 are separated by 110 nucleotides SMa0134 and SMa5002 are separated by 40 nucleotides
SMa0130: SMa0130 - fatty acid desaturase, at 69,601 to 70,668
SMa0130
SMa0132: SMa0132 - hypothetical protein, at 70,668 to 70,955
SMa0132
SMa0134: SMa0134 - hypothetical protein, at 71,066 to 71,671
SMa0134
SMa5002: SMa5002 - hypothetical protein, at 71,712 to 72,026
SMa5002
Position (kb) 70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 69.676 kb on + strand at 70.030 kb on + strand, within SMa0130 at 70.072 kb on + strand, within SMa0130 at 70.309 kb on + strand, within SMa0130 at 70.309 kb on + strand, within SMa0130 at 70.396 kb on + strand, within SMa0130 at 70.397 kb on - strand, within SMa0130 at 70.397 kb on - strand, within SMa0130 at 70.435 kb on - strand, within SMa0130 at 70.477 kb on + strand, within SMa0130 at 70.497 kb on - strand, within SMa0130 at 70.598 kb on + strand at 70.666 kb on + strand at 70.776 kb on + strand, within SMa0132 at 70.777 kb on - strand, within SMa0132 at 70.777 kb on - strand, within SMa0132 at 70.880 kb on + strand, within SMa0132 at 70.936 kb on + strand at 70.937 kb on - strand at 70.953 kb on + strand at 70.954 kb on - strand at 70.954 kb on - strand at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.167 kb on - strand, within SMa0134 at 71.167 kb on - strand, within SMa0134 at 71.167 kb on - strand, within SMa0134 at 71.292 kb on + strand, within SMa0134 at 71.293 kb on - strand, within SMa0134 at 71.420 kb on + strand, within SMa0134 at 71.420 kb on + strand, within SMa0134 at 71.496 kb on - strand, within SMa0134 at 71.496 kb on - strand, within SMa0134 at 71.640 kb on + strand at 71.660 kb on + strand at 71.660 kb on + strand at 71.672 kb on - strand at 71.714 kb on - strand at 71.714 kb on - strand at 71.725 kb on - strand at 71.828 kb on + strand, within SMa5002 at 71.828 kb on + strand, within SMa5002 at 71.828 kb on + strand, within SMa5002 at 71.828 kb on + strand, within SMa5002 at 71.829 kb on - strand, within SMa5002 at 71.926 kb on - strand, within SMa5002
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 69,676 + +0.1 70,030 + SMa0130 0.40 -0.2 70,072 + SMa0130 0.44 -0.6 70,309 + SMa0130 0.66 -0.8 70,309 + SMa0130 0.66 +0.1 70,396 + SMa0130 0.74 +0.3 70,397 - SMa0130 0.75 -0.6 70,397 - SMa0130 0.75 +0.1 70,435 - SMa0130 0.78 +0.7 70,477 + SMa0130 0.82 +0.5 70,497 - SMa0130 0.84 -0.5 70,598 + -1.5 70,666 + -2.0 70,776 + SMa0132 0.38 -0.3 70,777 - SMa0132 0.38 -2.4 70,777 - SMa0132 0.38 -2.2 70,880 + SMa0132 0.74 -1.2 70,936 + -1.4 70,937 - -0.6 70,953 + -0.2 70,954 - -1.8 70,954 - +1.0 71,166 + SMa0134 0.17 +0.5 71,166 + SMa0134 0.17 +0.5 71,166 + SMa0134 0.17 -0.1 71,166 + SMa0134 0.17 +0.2 71,166 + SMa0134 0.17 -0.6 71,167 - SMa0134 0.17 -2.9 71,167 - SMa0134 0.17 -0.1 71,167 - SMa0134 0.17 -0.2 71,292 + SMa0134 0.37 +0.5 71,293 - SMa0134 0.37 -0.0 71,420 + SMa0134 0.58 +0.5 71,420 + SMa0134 0.58 +0.3 71,496 - SMa0134 0.71 -0.8 71,496 - SMa0134 0.71 -1.6 71,640 + +0.7 71,660 + +1.4 71,660 + +0.8 71,672 - +0.1 71,714 - -0.0 71,714 - -1.5 71,725 - -0.5 71,828 + SMa5002 0.37 -1.1 71,828 + SMa5002 0.37 +0.5 71,828 + SMa5002 0.37 +1.9 71,828 + SMa5002 0.37 +0.0 71,829 - SMa5002 0.37 +0.3 71,926 - SMa5002 0.68 +0.3
Or see this region's nucleotide sequence