Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21082 and SM_b21083 are separated by 135 nucleotides SM_b21083 and SM_b21084 overlap by 4 nucleotides SM_b21084 and SM_b21085 are separated by 62 nucleotides SM_b21085 and SM_b21086 are separated by 425 nucleotides
SM_b21082: SM_b21082 - mannose-6-phosphate isomerase, GDP-mannose pyrophosphorylase, at 719,985 to 722,288
_b21082
SM_b21083: SM_b21083 - hypothetical protein, at 722,424 to 722,867
_b21083
SM_b21084: SM_b21084 - hypothetical protein, at 722,864 to 723,217
_b21084
SM_b21085: SM_b21085 - hypothetical protein, at 723,280 to 724,932
_b21085
SM_b21086: SM_b21086 - hypothetical protein, at 725,358 to 725,834
_b21086
Position (kb)
723
724
725 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 722.312 kb on + strand at 722.313 kb on - strand at 722.316 kb on + strand at 722.337 kb on - strand at 722.337 kb on - strand at 722.362 kb on - strand at 722.369 kb on - strand at 723.140 kb on + strand, within SM_b21084 at 723.140 kb on + strand, within SM_b21084 at 723.219 kb on + strand at 723.220 kb on - strand at 723.220 kb on - strand at 723.221 kb on + strand at 723.476 kb on - strand, within SM_b21085 at 724.062 kb on + strand, within SM_b21085 at 724.063 kb on - strand, within SM_b21085 at 724.063 kb on - strand, within SM_b21085 at 724.100 kb on - strand, within SM_b21085 at 724.100 kb on - strand, within SM_b21085 at 725.064 kb on + strand at 725.122 kb on + strand at 725.122 kb on + strand at 725.123 kb on - strand at 725.123 kb on - strand at 725.125 kb on + strand at 725.125 kb on + strand at 725.125 kb on + strand at 725.126 kb on - strand at 725.126 kb on - strand at 725.167 kb on - strand at 725.230 kb on + strand at 725.231 kb on - strand at 725.231 kb on - strand at 725.354 kb on + strand at 725.354 kb on + strand at 725.354 kb on + strand at 725.355 kb on - strand at 725.375 kb on - strand at 725.375 kb on - strand at 725.412 kb on + strand, within SM_b21086 at 725.412 kb on + strand, within SM_b21086 at 725.412 kb on + strand, within SM_b21086 at 725.413 kb on - strand, within SM_b21086 at 725.413 kb on - strand, within SM_b21086 at 725.413 kb on - strand, within SM_b21086 at 725.451 kb on + strand, within SM_b21086 at 725.452 kb on - strand, within SM_b21086 at 725.452 kb on - strand, within SM_b21086 at 725.452 kb on - strand, within SM_b21086 at 725.492 kb on - strand, within SM_b21086 at 725.496 kb on - strand, within SM_b21086 at 725.496 kb on - strand, within SM_b21086 at 725.506 kb on - strand, within SM_b21086 at 725.543 kb on - strand, within SM_b21086 at 725.543 kb on - strand, within SM_b21086 at 725.550 kb on + strand, within SM_b21086 at 725.550 kb on + strand, within SM_b21086 at 725.802 kb on + strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.823 kb on + strand at 725.827 kb on + strand at 725.854 kb on - strand at 725.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 722,312 + -3.8 722,313 - -0.3 722,316 + +0.7 722,337 - +0.5 722,337 - +0.7 722,362 - +0.4 722,369 - -2.3 723,140 + SM_b21084 0.78 +0.8 723,140 + SM_b21084 0.78 +1.3 723,219 + +0.0 723,220 - +0.4 723,220 - -0.1 723,221 + +1.1 723,476 - SM_b21085 0.12 +1.0 724,062 + SM_b21085 0.47 +1.1 724,063 - SM_b21085 0.47 -0.2 724,063 - SM_b21085 0.47 +1.2 724,100 - SM_b21085 0.50 -0.6 724,100 - SM_b21085 0.50 +0.8 725,064 + +0.3 725,122 + +0.2 725,122 + +0.3 725,123 - +0.8 725,123 - -0.5 725,125 + +0.2 725,125 + +0.8 725,125 + -0.8 725,126 - -0.4 725,126 - -0.1 725,167 - +1.8 725,230 + +1.0 725,231 - +0.5 725,231 - +0.3 725,354 + +0.3 725,354 + +0.2 725,354 + +0.1 725,355 - -0.6 725,375 - -1.2 725,375 - +1.5 725,412 + SM_b21086 0.11 -0.6 725,412 + SM_b21086 0.11 -0.7 725,412 + SM_b21086 0.11 +0.8 725,413 - SM_b21086 0.12 -0.2 725,413 - SM_b21086 0.12 -0.3 725,413 - SM_b21086 0.12 +1.1 725,451 + SM_b21086 0.19 -0.1 725,452 - SM_b21086 0.20 +1.2 725,452 - SM_b21086 0.20 +0.3 725,452 - SM_b21086 0.20 -3.1 725,492 - SM_b21086 0.28 -0.5 725,496 - SM_b21086 0.29 +0.1 725,496 - SM_b21086 0.29 -0.6 725,506 - SM_b21086 0.31 +0.5 725,543 - SM_b21086 0.39 -0.3 725,543 - SM_b21086 0.39 +0.0 725,550 + SM_b21086 0.40 +0.5 725,550 + SM_b21086 0.40 +0.1 725,802 + +0.8 725,803 - +1.2 725,803 - -1.1 725,803 - -3.3 725,803 - +0.6 725,803 - -1.1 725,823 + -1.2 725,827 + -0.5 725,854 - +1.3 725,877 - -0.0
Or see this region's nucleotide sequence