Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21082 and SM_b21083 are separated by 135 nucleotides SM_b21083 and SM_b21084 overlap by 4 nucleotides SM_b21084 and SM_b21085 are separated by 62 nucleotides
SM_b21082: SM_b21082 - mannose-6-phosphate isomerase, GDP-mannose pyrophosphorylase, at 719,985 to 722,288
_b21082
SM_b21083: SM_b21083 - hypothetical protein, at 722,424 to 722,867
_b21083
SM_b21084: SM_b21084 - hypothetical protein, at 722,864 to 723,217
_b21084
SM_b21085: SM_b21085 - hypothetical protein, at 723,280 to 724,932
_b21085
Position (kb)
722
723
724 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 721.866 kb on + strand, within SM_b21082 at 721.867 kb on - strand, within SM_b21082 at 722.072 kb on + strand at 722.155 kb on + strand at 722.155 kb on + strand at 722.155 kb on + strand at 722.160 kb on + strand at 722.160 kb on + strand at 722.160 kb on + strand at 722.160 kb on + strand at 722.160 kb on + strand at 722.160 kb on + strand at 722.160 kb on + strand at 722.160 kb on + strand at 722.161 kb on - strand at 722.161 kb on - strand at 722.188 kb on - strand at 722.214 kb on + strand at 722.273 kb on + strand at 722.273 kb on + strand at 722.312 kb on + strand at 722.313 kb on - strand at 722.316 kb on + strand at 722.337 kb on - strand at 722.337 kb on - strand at 722.362 kb on - strand at 722.369 kb on - strand at 723.140 kb on + strand, within SM_b21084 at 723.140 kb on + strand, within SM_b21084 at 723.219 kb on + strand at 723.220 kb on - strand at 723.220 kb on - strand at 723.221 kb on + strand at 723.476 kb on - strand, within SM_b21085 at 724.062 kb on + strand, within SM_b21085 at 724.063 kb on - strand, within SM_b21085 at 724.063 kb on - strand, within SM_b21085 at 724.100 kb on - strand, within SM_b21085 at 724.100 kb on - strand, within SM_b21085
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 721,866 + SM_b21082 0.82 -0.5 721,867 - SM_b21082 0.82 -0.7 722,072 + -0.2 722,155 + +0.0 722,155 + -0.2 722,155 + +0.9 722,160 + -1.1 722,160 + +1.0 722,160 + +0.0 722,160 + +0.3 722,160 + -0.5 722,160 + -0.2 722,160 + +0.3 722,160 + +0.9 722,161 - +0.3 722,161 - +0.3 722,188 - +0.2 722,214 + -0.5 722,273 + -0.2 722,273 + -0.9 722,312 + -3.8 722,313 - -0.3 722,316 + +0.7 722,337 - +0.5 722,337 - +0.7 722,362 - +0.4 722,369 - -2.3 723,140 + SM_b21084 0.78 +0.8 723,140 + SM_b21084 0.78 +1.3 723,219 + +0.0 723,220 - +0.4 723,220 - -0.1 723,221 + +1.1 723,476 - SM_b21085 0.12 +1.0 724,062 + SM_b21085 0.47 +1.1 724,063 - SM_b21085 0.47 -0.2 724,063 - SM_b21085 0.47 +1.2 724,100 - SM_b21085 0.50 -0.6 724,100 - SM_b21085 0.50 +0.8
Or see this region's nucleotide sequence