Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20975 and SM_b20976 are separated by 73 nucleotides SM_b20976 and SM_b20977 are separated by 30 nucleotides SM_b20977 and SM_b20978 are separated by 234 nucleotides SM_b20978 and SM_b20979 are separated by 87 nucleotides
SM_b20975: SM_b20975 - amino acid uptake ABC transporter permease, at 1,207,152 to 1,208,801
_b20975
SM_b20976: SM_b20976 - amino acid uptake ABC transporter substrate-binding protein precursor, at 1,208,875 to 1,209,621
_b20976
SM_b20977: SM_b20977 - LacI family transcriptional regulator, at 1,209,652 to 1,210,719
_b20977
SM_b20978: SM_b20978 - hypothetical protein, at 1,210,954 to 1,211,505
_b20978
SM_b20979: SM_b20979 - large C4-dicarboxylate uptake permease, DedA family protein, at 1,211,593 to 1,212,906
_b20979
Position (kb)
1209
1210
1211 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1208.734 kb on + strand at 1208.735 kb on - strand at 1208.735 kb on - strand at 1208.735 kb on - strand at 1208.876 kb on + strand at 1208.876 kb on + strand at 1208.877 kb on - strand at 1209.137 kb on + strand, within SM_b20976 at 1209.271 kb on + strand, within SM_b20976 at 1209.271 kb on + strand, within SM_b20976 at 1209.293 kb on + strand, within SM_b20976 at 1209.294 kb on - strand, within SM_b20976 at 1209.294 kb on - strand, within SM_b20976 at 1209.294 kb on - strand, within SM_b20976 at 1209.461 kb on - strand, within SM_b20976 at 1209.538 kb on - strand, within SM_b20976 at 1209.667 kb on + strand at 1209.806 kb on + strand, within SM_b20977 at 1209.806 kb on + strand, within SM_b20977 at 1209.807 kb on - strand, within SM_b20977 at 1209.833 kb on + strand, within SM_b20977 at 1209.834 kb on - strand, within SM_b20977 at 1209.834 kb on - strand, within SM_b20977 at 1210.011 kb on + strand, within SM_b20977 at 1210.313 kb on - strand, within SM_b20977 at 1210.403 kb on + strand, within SM_b20977 at 1210.450 kb on - strand, within SM_b20977 at 1210.450 kb on - strand, within SM_b20977 at 1210.450 kb on - strand, within SM_b20977 at 1210.451 kb on + strand, within SM_b20977 at 1210.452 kb on - strand, within SM_b20977 at 1210.452 kb on - strand, within SM_b20977 at 1210.472 kb on + strand, within SM_b20977 at 1210.472 kb on + strand, within SM_b20977 at 1210.472 kb on + strand, within SM_b20977 at 1210.472 kb on + strand, within SM_b20977 at 1210.472 kb on + strand, within SM_b20977 at 1210.472 kb on + strand, within SM_b20977 at 1210.473 kb on - strand, within SM_b20977 at 1210.656 kb on - strand at 1210.806 kb on + strand at 1210.806 kb on + strand at 1210.806 kb on + strand at 1210.806 kb on + strand at 1210.807 kb on - strand at 1210.807 kb on - strand at 1210.807 kb on - strand at 1210.808 kb on + strand at 1210.823 kb on + strand at 1210.956 kb on - strand at 1210.956 kb on - strand at 1211.021 kb on + strand, within SM_b20978 at 1211.057 kb on + strand, within SM_b20978 at 1211.057 kb on + strand, within SM_b20978 at 1211.088 kb on - strand, within SM_b20978 at 1211.171 kb on + strand, within SM_b20978 at 1211.172 kb on - strand, within SM_b20978 at 1211.177 kb on + strand, within SM_b20978 at 1211.203 kb on - strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.300 kb on + strand, within SM_b20978 at 1211.301 kb on - strand, within SM_b20978 at 1211.326 kb on - strand, within SM_b20978 at 1211.326 kb on - strand, within SM_b20978 at 1211.326 kb on - strand, within SM_b20978 at 1211.341 kb on - strand, within SM_b20978 at 1211.401 kb on + strand, within SM_b20978 at 1211.513 kb on - strand at 1211.535 kb on + strand at 1211.581 kb on + strand at 1211.582 kb on - strand at 1211.582 kb on - strand at 1211.615 kb on + strand at 1211.615 kb on + strand at 1211.615 kb on + strand at 1211.615 kb on + strand at 1211.693 kb on + strand at 1211.693 kb on + strand at 1211.694 kb on - strand at 1211.714 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 1,208,734 + +0.8 1,208,735 - +0.8 1,208,735 - +2.4 1,208,735 - -0.2 1,208,876 + +0.6 1,208,876 + -0.1 1,208,877 - -1.3 1,209,137 + SM_b20976 0.35 -0.5 1,209,271 + SM_b20976 0.53 +0.6 1,209,271 + SM_b20976 0.53 +0.2 1,209,293 + SM_b20976 0.56 -0.8 1,209,294 - SM_b20976 0.56 -1.4 1,209,294 - SM_b20976 0.56 -1.9 1,209,294 - SM_b20976 0.56 -0.2 1,209,461 - SM_b20976 0.78 -3.3 1,209,538 - SM_b20976 0.89 -0.7 1,209,667 + -0.4 1,209,806 + SM_b20977 0.14 -0.4 1,209,806 + SM_b20977 0.14 -0.3 1,209,807 - SM_b20977 0.15 +0.7 1,209,833 + SM_b20977 0.17 +0.6 1,209,834 - SM_b20977 0.17 +0.5 1,209,834 - SM_b20977 0.17 -1.5 1,210,011 + SM_b20977 0.34 -2.1 1,210,313 - SM_b20977 0.62 +0.4 1,210,403 + SM_b20977 0.70 -0.2 1,210,450 - SM_b20977 0.75 +0.4 1,210,450 - SM_b20977 0.75 +1.7 1,210,450 - SM_b20977 0.75 +0.3 1,210,451 + SM_b20977 0.75 -0.0 1,210,452 - SM_b20977 0.75 +0.3 1,210,452 - SM_b20977 0.75 +1.3 1,210,472 + SM_b20977 0.77 -0.1 1,210,472 + SM_b20977 0.77 -0.2 1,210,472 + SM_b20977 0.77 -0.3 1,210,472 + SM_b20977 0.77 -0.4 1,210,472 + SM_b20977 0.77 -0.2 1,210,472 + SM_b20977 0.77 -0.1 1,210,473 - SM_b20977 0.77 +0.2 1,210,656 - +0.5 1,210,806 + -0.8 1,210,806 + -2.0 1,210,806 + -1.8 1,210,806 + +0.5 1,210,807 - -1.2 1,210,807 - -0.5 1,210,807 - -0.1 1,210,808 + -0.7 1,210,823 + -0.3 1,210,956 - +0.1 1,210,956 - -0.7 1,211,021 + SM_b20978 0.12 -1.5 1,211,057 + SM_b20978 0.19 -0.9 1,211,057 + SM_b20978 0.19 +0.0 1,211,088 - SM_b20978 0.24 -0.5 1,211,171 + SM_b20978 0.39 -0.1 1,211,172 - SM_b20978 0.39 +0.4 1,211,177 + SM_b20978 0.40 -0.4 1,211,203 - SM_b20978 0.45 -2.9 1,211,300 + SM_b20978 0.63 +0.5 1,211,300 + SM_b20978 0.63 +0.3 1,211,300 + SM_b20978 0.63 -0.9 1,211,300 + SM_b20978 0.63 -0.2 1,211,300 + SM_b20978 0.63 -1.1 1,211,300 + SM_b20978 0.63 +0.6 1,211,300 + SM_b20978 0.63 +0.0 1,211,300 + SM_b20978 0.63 -0.4 1,211,301 - SM_b20978 0.63 +0.2 1,211,326 - SM_b20978 0.67 +1.5 1,211,326 - SM_b20978 0.67 -1.5 1,211,326 - SM_b20978 0.67 -1.5 1,211,341 - SM_b20978 0.70 -1.1 1,211,401 + SM_b20978 0.81 -0.1 1,211,513 - -1.2 1,211,535 + +0.3 1,211,581 + -0.4 1,211,582 - +0.5 1,211,582 - -0.0 1,211,615 + +2.0 1,211,615 + -0.5 1,211,615 + +0.6 1,211,615 + -1.0 1,211,693 + +0.8 1,211,693 + -0.3 1,211,694 - -1.5 1,211,714 + -0.6
Or see this region's nucleotide sequence