Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20209 and SM_b20210 are separated by 54 nucleotides SM_b20210 and SM_b20211 are separated by 141 nucleotides SM_b20211 and SM_b20212 are separated by 187 nucleotides SM_b20212 and SM_b20213 are separated by 37 nucleotides
SM_b20209: SM_b20209 - hypothetical protein, at 216,993 to 217,232
_b20209
SM_b20210: SM_b20210 - oxidoreductase, at 217,287 to 218,000
_b20210
SM_b20211: SM_b20211 - transcriptional regulator, at 218,142 to 219,074
_b20211
SM_b20212: SM_b20212 - hypothetical protein, at 219,262 to 219,942
_b20212
SM_b20213: SM_b20213 - hypothetical protein, at 219,980 to 221,863
_b20213
Position (kb) 218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 217.215 kb on + strand at 217.215 kb on + strand at 217.216 kb on - strand at 217.227 kb on - strand at 217.279 kb on + strand at 217.279 kb on + strand at 217.279 kb on + strand at 217.465 kb on - strand, within SM_b20210 at 217.623 kb on - strand, within SM_b20210 at 217.860 kb on + strand, within SM_b20210 at 218.322 kb on + strand, within SM_b20211 at 218.322 kb on + strand, within SM_b20211 at 218.322 kb on + strand, within SM_b20211 at 218.322 kb on + strand, within SM_b20211 at 218.323 kb on - strand, within SM_b20211 at 218.323 kb on - strand, within SM_b20211 at 218.326 kb on + strand, within SM_b20211 at 218.326 kb on + strand, within SM_b20211 at 218.327 kb on - strand, within SM_b20211 at 218.327 kb on - strand, within SM_b20211 at 218.341 kb on + strand, within SM_b20211 at 218.347 kb on + strand, within SM_b20211 at 218.348 kb on - strand, within SM_b20211 at 218.757 kb on + strand, within SM_b20211 at 218.757 kb on + strand, within SM_b20211 at 218.758 kb on - strand, within SM_b20211 at 218.758 kb on - strand, within SM_b20211 at 218.758 kb on - strand, within SM_b20211 at 218.881 kb on + strand, within SM_b20211 at 218.882 kb on - strand, within SM_b20211 at 219.074 kb on + strand at 219.190 kb on - strand at 219.191 kb on + strand at 219.210 kb on + strand at 219.219 kb on + strand at 219.359 kb on - strand, within SM_b20212 at 219.361 kb on + strand, within SM_b20212 at 219.362 kb on - strand, within SM_b20212 at 219.362 kb on - strand, within SM_b20212 at 219.362 kb on - strand, within SM_b20212 at 219.514 kb on - strand, within SM_b20212 at 219.524 kb on + strand, within SM_b20212 at 219.524 kb on + strand, within SM_b20212 at 219.525 kb on - strand, within SM_b20212 at 219.562 kb on + strand, within SM_b20212 at 219.563 kb on - strand, within SM_b20212 at 219.634 kb on + strand, within SM_b20212 at 219.635 kb on - strand, within SM_b20212 at 219.826 kb on + strand, within SM_b20212 at 219.940 kb on + strand at 219.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 217,215 + +0.8 217,215 + -0.1 217,216 - -2.0 217,227 - +0.0 217,279 + +1.2 217,279 + -0.2 217,279 + -2.8 217,465 - SM_b20210 0.25 -0.3 217,623 - SM_b20210 0.47 -0.1 217,860 + SM_b20210 0.80 +0.5 218,322 + SM_b20211 0.19 +0.6 218,322 + SM_b20211 0.19 -1.0 218,322 + SM_b20211 0.19 -0.5 218,322 + SM_b20211 0.19 -0.4 218,323 - SM_b20211 0.19 -1.7 218,323 - SM_b20211 0.19 -0.6 218,326 + SM_b20211 0.20 +0.0 218,326 + SM_b20211 0.20 +0.5 218,327 - SM_b20211 0.20 -1.0 218,327 - SM_b20211 0.20 -0.6 218,341 + SM_b20211 0.21 +0.0 218,347 + SM_b20211 0.22 -0.7 218,348 - SM_b20211 0.22 -2.6 218,757 + SM_b20211 0.66 -0.2 218,757 + SM_b20211 0.66 -0.2 218,758 - SM_b20211 0.66 -0.3 218,758 - SM_b20211 0.66 -1.4 218,758 - SM_b20211 0.66 +0.2 218,881 + SM_b20211 0.79 -0.0 218,882 - SM_b20211 0.79 +0.0 219,074 + -0.3 219,190 - +0.1 219,191 + -1.2 219,210 + +0.3 219,219 + +0.8 219,359 - SM_b20212 0.14 +0.3 219,361 + SM_b20212 0.15 -0.5 219,362 - SM_b20212 0.15 +0.3 219,362 - SM_b20212 0.15 -1.2 219,362 - SM_b20212 0.15 -0.0 219,514 - SM_b20212 0.37 +0.5 219,524 + SM_b20212 0.38 -2.8 219,524 + SM_b20212 0.38 -0.1 219,525 - SM_b20212 0.39 -0.1 219,562 + SM_b20212 0.44 +0.4 219,563 - SM_b20212 0.44 -1.3 219,634 + SM_b20212 0.55 -1.0 219,635 - SM_b20212 0.55 -0.1 219,826 + SM_b20212 0.83 -3.7 219,940 + +0.6 219,971 - -0.0
Or see this region's nucleotide sequence