Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20168 and SM_b20169 are separated by 128 nucleotides SM_b20169 and SM_b20170 are separated by 169 nucleotides SM_b20170 and SM_b20171 overlap by 4 nucleotides SM_b20171 and SM_b20172 are separated by 13 nucleotides
SM_b20168: SM_b20168 - hypothetical protein, at 186,369 to 187,466
_b20168
SM_b20169: SM_b20169 - hypothetical protein, at 187,595 to 188,236
_b20169
SM_b20170: SM_b20170 - glutathione-dependent formaldehyde dehydrogenase, at 188,406 to 189,533
_b20170
SM_b20171: SM_b20171 - carboxylesterase, at 189,530 to 190,372
_b20171
SM_b20172: SM_b20172 - cytochrome c protein, at 190,386 to 190,796
_b20172
Position (kb) 188
189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 187.465 kb on - strand at 187.512 kb on + strand at 187.674 kb on + strand, within SM_b20169 at 187.674 kb on + strand, within SM_b20169 at 187.675 kb on - strand, within SM_b20169 at 187.675 kb on - strand, within SM_b20169 at 187.675 kb on - strand, within SM_b20169 at 187.675 kb on - strand, within SM_b20169 at 187.675 kb on - strand, within SM_b20169 at 187.675 kb on - strand, within SM_b20169 at 187.675 kb on - strand, within SM_b20169 at 187.701 kb on + strand, within SM_b20169 at 187.882 kb on + strand, within SM_b20169 at 188.101 kb on + strand, within SM_b20169 at 188.177 kb on + strand at 188.178 kb on - strand at 188.178 kb on - strand at 188.269 kb on + strand at 188.271 kb on + strand at 188.271 kb on + strand at 188.272 kb on - strand at 188.272 kb on - strand at 188.313 kb on + strand at 188.313 kb on + strand at 188.314 kb on - strand at 188.349 kb on + strand at 188.350 kb on - strand at 188.380 kb on + strand at 188.384 kb on + strand at 188.384 kb on + strand at 188.385 kb on - strand at 188.389 kb on + strand at 188.389 kb on + strand at 188.389 kb on + strand at 188.390 kb on - strand at 189.528 kb on + strand at 189.528 kb on + strand at 189.552 kb on + strand at 189.638 kb on + strand, within SM_b20171 at 189.639 kb on - strand, within SM_b20171 at 189.684 kb on - strand, within SM_b20171 at 189.822 kb on - strand, within SM_b20171 at 189.848 kb on + strand, within SM_b20171 at 189.849 kb on - strand, within SM_b20171 at 189.884 kb on + strand, within SM_b20171 at 189.893 kb on + strand at 189.893 kb on + strand, within SM_b20171 at 189.893 kb on + strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.894 kb on - strand, within SM_b20171 at 189.899 kb on + strand, within SM_b20171 at 189.899 kb on + strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.900 kb on - strand, within SM_b20171 at 189.902 kb on - strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.178 kb on - strand, within SM_b20171 at 190.304 kb on + strand at 190.307 kb on + strand at 190.307 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 187,465 - -1.0 187,512 + -0.9 187,674 + SM_b20169 0.12 -1.3 187,674 + SM_b20169 0.12 -0.3 187,675 - SM_b20169 0.12 -0.1 187,675 - SM_b20169 0.12 +3.6 187,675 - SM_b20169 0.12 -2.0 187,675 - SM_b20169 0.12 +0.2 187,675 - SM_b20169 0.12 -0.3 187,675 - SM_b20169 0.12 +0.3 187,675 - SM_b20169 0.12 +0.6 187,701 + SM_b20169 0.17 +0.1 187,882 + SM_b20169 0.45 +0.0 188,101 + SM_b20169 0.79 +0.1 188,177 + -0.1 188,178 - -1.2 188,178 - -0.4 188,269 + -2.8 188,271 + -0.9 188,271 + -0.4 188,272 - +0.5 188,272 - +0.8 188,313 + -2.0 188,313 + -1.9 188,314 - +1.1 188,349 + +2.6 188,350 - +1.3 188,380 + -3.1 188,384 + -2.6 188,384 + -0.4 188,385 - -0.5 188,389 + +0.4 188,389 + +0.2 188,389 + -0.0 188,390 - +0.4 189,528 + +0.2 189,528 + -0.1 189,552 + +0.5 189,638 + SM_b20171 0.13 -0.4 189,639 - SM_b20171 0.13 +1.0 189,684 - SM_b20171 0.18 -0.3 189,822 - SM_b20171 0.35 -2.6 189,848 + SM_b20171 0.38 +0.0 189,849 - SM_b20171 0.38 -1.1 189,884 + SM_b20171 0.42 -0.4 189,893 + -0.8 189,893 + SM_b20171 0.43 -0.7 189,893 + SM_b20171 0.43 +0.5 189,894 - SM_b20171 0.43 +0.7 189,894 - SM_b20171 0.43 -0.0 189,894 - SM_b20171 0.43 +0.1 189,894 - SM_b20171 0.43 +2.8 189,894 - SM_b20171 0.43 -1.0 189,894 - SM_b20171 0.43 +0.3 189,894 - SM_b20171 0.43 +0.4 189,899 + SM_b20171 0.44 -0.4 189,899 + SM_b20171 0.44 -1.2 189,900 - SM_b20171 0.44 -1.4 189,900 - SM_b20171 0.44 -2.2 189,900 - SM_b20171 0.44 -0.7 189,900 - SM_b20171 0.44 +0.2 189,902 - SM_b20171 0.44 -1.1 190,133 + SM_b20171 0.72 -3.4 190,133 + SM_b20171 0.72 +0.4 190,133 + SM_b20171 0.72 -0.7 190,178 - SM_b20171 0.77 -0.2 190,304 + +0.8 190,307 + -0.7 190,307 + -0.7
Or see this region's nucleotide sequence