Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b22005 and SM_b20161 overlap by 4 nucleotides SM_b20161 and SM_b20162 are separated by 193 nucleotides SM_b20162 and SM_b20163 are separated by 44 nucleotides
SM_b22005: SM_b22005 - hypothetical protein, at 179,168 to 179,536
_b22005
SM_b20161: SM_b20161 - hypothetical protein, at 179,533 to 179,883
_b20161
SM_b20162: SM_b20162 - transcriptional regulator, at 180,077 to 180,766
_b20162
SM_b20163: SM_b20163 - hypothetical protein, at 180,811 to 181,980
_b20163
Position (kb) 180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 179.088 kb on + strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.497 kb on - strand, within SM_b22005 at 179.599 kb on + strand, within SM_b20161 at 179.601 kb on + strand, within SM_b20161 at 179.601 kb on + strand, within SM_b20161 at 179.602 kb on - strand, within SM_b20161 at 179.605 kb on - strand, within SM_b20161 at 179.670 kb on + strand, within SM_b20161 at 179.671 kb on - strand, within SM_b20161 at 179.671 kb on - strand, within SM_b20161 at 179.765 kb on + strand, within SM_b20161 at 179.766 kb on - strand, within SM_b20161 at 179.910 kb on + strand at 179.910 kb on + strand at 179.911 kb on - strand at 180.059 kb on + strand at 180.060 kb on - strand at 180.149 kb on + strand, within SM_b20162 at 180.150 kb on - strand, within SM_b20162 at 180.190 kb on + strand, within SM_b20162 at 180.190 kb on + strand, within SM_b20162 at 180.304 kb on - strand, within SM_b20162 at 180.442 kb on + strand, within SM_b20162 at 180.443 kb on - strand, within SM_b20162 at 180.466 kb on + strand, within SM_b20162 at 180.485 kb on + strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.501 kb on - strand, within SM_b20162 at 180.673 kb on + strand, within SM_b20162 at 180.673 kb on + strand, within SM_b20162 at 180.673 kb on + strand, within SM_b20162 at 180.674 kb on - strand, within SM_b20162 at 180.741 kb on + strand at 180.742 kb on - strand at 181.261 kb on - strand, within SM_b20163 at 181.261 kb on - strand, within SM_b20163 at 181.535 kb on - strand, within SM_b20163 at 181.535 kb on - strand, within SM_b20163 at 181.535 kb on - strand, within SM_b20163 at 181.621 kb on + strand, within SM_b20163 at 181.621 kb on + strand, within SM_b20163 at 181.622 kb on - strand, within SM_b20163
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 179,088 + +0.2 179,089 - +1.3 179,089 - -0.2 179,089 - +1.8 179,089 - +1.0 179,089 - -2.3 179,089 - +0.3 179,497 - SM_b22005 0.89 +0.6 179,599 + SM_b20161 0.19 +1.3 179,601 + SM_b20161 0.19 +0.1 179,601 + SM_b20161 0.19 -3.0 179,602 - SM_b20161 0.20 +2.4 179,605 - SM_b20161 0.21 -1.6 179,670 + SM_b20161 0.39 +0.4 179,671 - SM_b20161 0.39 -0.0 179,671 - SM_b20161 0.39 -0.1 179,765 + SM_b20161 0.66 -0.0 179,766 - SM_b20161 0.66 +1.3 179,910 + +0.1 179,910 + +0.1 179,911 - +0.8 180,059 + -0.2 180,060 - +0.9 180,149 + SM_b20162 0.10 +2.4 180,150 - SM_b20162 0.11 +0.7 180,190 + SM_b20162 0.16 +2.3 180,190 + SM_b20162 0.16 -0.3 180,304 - SM_b20162 0.33 +1.1 180,442 + SM_b20162 0.53 +0.7 180,443 - SM_b20162 0.53 -0.4 180,466 + SM_b20162 0.56 +1.9 180,485 + SM_b20162 0.59 +0.3 180,486 - SM_b20162 0.59 +0.0 180,486 - SM_b20162 0.59 -0.1 180,486 - SM_b20162 0.59 +0.7 180,486 - SM_b20162 0.59 +2.1 180,486 - SM_b20162 0.59 +0.2 180,501 - SM_b20162 0.61 +0.6 180,673 + SM_b20162 0.86 -2.2 180,673 + SM_b20162 0.86 +0.1 180,673 + SM_b20162 0.86 +0.0 180,674 - SM_b20162 0.87 -0.4 180,741 + +0.5 180,742 - -0.7 181,261 - SM_b20163 0.38 -0.1 181,261 - SM_b20163 0.38 -0.3 181,535 - SM_b20163 0.62 +0.1 181,535 - SM_b20163 0.62 +0.6 181,535 - SM_b20163 0.62 -0.1 181,621 + SM_b20163 0.69 -0.0 181,621 + SM_b20163 0.69 +0.8 181,622 - SM_b20163 0.69 -3.1
Or see this region's nucleotide sequence