Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20088 and SM_b20089 are separated by 18 nucleotides SM_b20089 and SM_b20090 are separated by 3 nucleotides SM_b20090 and SM_b20091 are separated by 115 nucleotides SM_b20091 and SM_b20092 are separated by 85 nucleotides SM_b20092 and SM_b20093 are separated by 37 nucleotides
SM_b20088: SM_b20088 - hypothetical protein, at 96,557 to 98,533
_b20088
SM_b20089: SM_b20089 - hypothetical protein, at 98,552 to 98,875
_b20089
SM_b20090: SM_b20090 - hypothetical protein, at 98,879 to 99,070
_b20090
SM_b20091: SM_b20091 - hypothetical protein, at 99,186 to 99,692
_b20091
SM_b20092: SM_b20092 - hypothetical protein, at 99,778 to 100,536
_b20092
SM_b20093: SM_b20093 - hypothetical protein, at 100,574 to 101,515
_b20093
Position (kb) 99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 98.286 kb on + strand, within SM_b20088 at 98.287 kb on - strand, within SM_b20088 at 98.405 kb on + strand at 98.493 kb on + strand at 98.493 kb on + strand at 98.494 kb on - strand at 98.494 kb on - strand at 98.553 kb on + strand at 98.554 kb on - strand at 98.554 kb on - strand at 98.800 kb on - strand, within SM_b20089 at 98.800 kb on - strand, within SM_b20089 at 98.853 kb on + strand at 98.854 kb on - strand at 98.854 kb on - strand at 98.855 kb on + strand at 98.856 kb on - strand at 98.880 kb on + strand at 99.180 kb on + strand at 99.192 kb on - strand at 99.404 kb on + strand, within SM_b20091 at 99.405 kb on - strand, within SM_b20091 at 99.489 kb on + strand, within SM_b20091 at 99.491 kb on + strand, within SM_b20091 at 99.491 kb on + strand, within SM_b20091 at 99.491 kb on + strand, within SM_b20091 at 99.492 kb on - strand, within SM_b20091 at 99.492 kb on - strand, within SM_b20091 at 99.503 kb on + strand, within SM_b20091 at 99.504 kb on - strand, within SM_b20091 at 99.507 kb on + strand, within SM_b20091 at 99.779 kb on + strand at 99.780 kb on - strand at 99.780 kb on - strand at 99.780 kb on - strand at 99.880 kb on + strand, within SM_b20092 at 99.880 kb on + strand, within SM_b20092 at 99.881 kb on - strand, within SM_b20092 at 99.881 kb on - strand, within SM_b20092 at 100.001 kb on + strand, within SM_b20092 at 100.002 kb on - strand, within SM_b20092 at 100.274 kb on + strand, within SM_b20092 at 100.325 kb on - strand, within SM_b20092 at 100.342 kb on - strand, within SM_b20092 at 100.354 kb on + strand at 100.354 kb on + strand, within SM_b20092 at 100.487 kb on + strand at 100.641 kb on - strand at 100.661 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 98,286 + SM_b20088 0.87 +0.2 98,287 - SM_b20088 0.88 -0.6 98,405 + +2.0 98,493 + -0.3 98,493 + -0.3 98,494 - +0.8 98,494 - -0.5 98,553 + -0.4 98,554 - +0.1 98,554 - -0.1 98,800 - SM_b20089 0.77 -0.6 98,800 - SM_b20089 0.77 +0.2 98,853 + +1.3 98,854 - -0.4 98,854 - +1.7 98,855 + +0.9 98,856 - +0.3 98,880 + +0.4 99,180 + +0.1 99,192 - -0.2 99,404 + SM_b20091 0.43 -1.0 99,405 - SM_b20091 0.43 -2.2 99,489 + SM_b20091 0.60 +0.1 99,491 + SM_b20091 0.60 -2.1 99,491 + SM_b20091 0.60 +1.4 99,491 + SM_b20091 0.60 +0.2 99,492 - SM_b20091 0.60 -0.6 99,492 - SM_b20091 0.60 -0.2 99,503 + SM_b20091 0.63 -0.6 99,504 - SM_b20091 0.63 -0.2 99,507 + SM_b20091 0.63 -1.3 99,779 + +2.6 99,780 - +0.1 99,780 - -1.3 99,780 - -0.5 99,880 + SM_b20092 0.13 -0.7 99,880 + SM_b20092 0.13 +1.0 99,881 - SM_b20092 0.14 +0.3 99,881 - SM_b20092 0.14 -0.0 100,001 + SM_b20092 0.29 +0.3 100,002 - SM_b20092 0.30 -2.9 100,274 + SM_b20092 0.65 +3.3 100,325 - SM_b20092 0.72 +0.5 100,342 - SM_b20092 0.74 -0.9 100,354 + -0.7 100,354 + SM_b20092 0.76 +0.8 100,487 + -0.6 100,641 - -0.1 100,661 - +0.1
Or see this region's nucleotide sequence