Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20030 and SM_b20031 are separated by 25 nucleotides SM_b20031 and SM_b20032 are separated by 96 nucleotides SM_b20032 and SM_b20033 are separated by 389 nucleotides
SM_b20030: SM_b20030 - RNA polymerase sigma factor SigJ, at 41,423 to 42,280
_b20030
SM_b20031: SM_b20031 - hypothetical protein, at 42,306 to 42,554
_b20031
SM_b20032: SM_b20032 - hypothetical protein, at 42,651 to 43,385
_b20032
SM_b20033: SM_b20033 - transcriptional regulator, at 43,775 to 44,437
_b20033
Position (kb) 42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 41.666 kb on + strand, within SM_b20030 at 41.666 kb on + strand, within SM_b20030 at 41.666 kb on + strand, within SM_b20030 at 41.666 kb on + strand, within SM_b20030 at 41.667 kb on - strand, within SM_b20030 at 41.667 kb on - strand, within SM_b20030 at 42.239 kb on + strand at 42.239 kb on + strand at 42.307 kb on + strand at 42.372 kb on - strand, within SM_b20031 at 42.372 kb on - strand, within SM_b20031 at 42.372 kb on - strand, within SM_b20031 at 42.372 kb on - strand, within SM_b20031 at 42.500 kb on - strand, within SM_b20031 at 42.500 kb on - strand, within SM_b20031 at 42.583 kb on + strand at 42.583 kb on + strand at 42.584 kb on - strand at 42.776 kb on - strand, within SM_b20032 at 42.825 kb on + strand, within SM_b20032 at 42.825 kb on + strand, within SM_b20032 at 42.826 kb on - strand, within SM_b20032 at 43.053 kb on - strand, within SM_b20032 at 43.275 kb on + strand, within SM_b20032 at 43.275 kb on + strand, within SM_b20032 at 43.275 kb on + strand, within SM_b20032 at 43.276 kb on - strand, within SM_b20032 at 43.276 kb on - strand, within SM_b20032 at 43.276 kb on - strand, within SM_b20032 at 43.358 kb on - strand at 43.358 kb on - strand at 43.358 kb on - strand at 43.537 kb on + strand at 43.537 kb on + strand at 43.538 kb on - strand at 43.538 kb on - strand at 43.538 kb on - strand at 43.675 kb on - strand at 43.777 kb on - strand at 43.777 kb on - strand at 43.813 kb on - strand at 43.813 kb on - strand at 44.068 kb on + strand, within SM_b20033 at 44.068 kb on + strand, within SM_b20033 at 44.250 kb on + strand, within SM_b20033 at 44.277 kb on + strand, within SM_b20033 at 44.277 kb on + strand, within SM_b20033 at 44.280 kb on + strand, within SM_b20033
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 41,666 + SM_b20030 0.28 -0.1 41,666 + SM_b20030 0.28 -2.4 41,666 + SM_b20030 0.28 -0.4 41,666 + SM_b20030 0.28 +0.1 41,667 - SM_b20030 0.28 -1.0 41,667 - SM_b20030 0.28 -0.0 42,239 + +0.5 42,239 + +0.6 42,307 + +0.3 42,372 - SM_b20031 0.27 -0.5 42,372 - SM_b20031 0.27 -1.1 42,372 - SM_b20031 0.27 +2.8 42,372 - SM_b20031 0.27 -0.2 42,500 - SM_b20031 0.78 -1.5 42,500 - SM_b20031 0.78 -2.4 42,583 + +0.2 42,583 + -0.6 42,584 - -1.0 42,776 - SM_b20032 0.17 -0.2 42,825 + SM_b20032 0.24 -0.1 42,825 + SM_b20032 0.24 -0.7 42,826 - SM_b20032 0.24 +0.9 43,053 - SM_b20032 0.55 +1.0 43,275 + SM_b20032 0.85 +0.4 43,275 + SM_b20032 0.85 +0.4 43,275 + SM_b20032 0.85 +0.2 43,276 - SM_b20032 0.85 -0.2 43,276 - SM_b20032 0.85 +0.5 43,276 - SM_b20032 0.85 +0.4 43,358 - +1.1 43,358 - +0.2 43,358 - -0.1 43,537 + +0.8 43,537 + +0.2 43,538 - -2.9 43,538 - +0.6 43,538 - -2.3 43,675 - -2.5 43,777 - -1.4 43,777 - +0.9 43,813 - +0.8 43,813 - +0.2 44,068 + SM_b20033 0.44 -0.0 44,068 + SM_b20033 0.44 -0.7 44,250 + SM_b20033 0.72 -0.6 44,277 + SM_b20033 0.76 -2.1 44,277 + SM_b20033 0.76 +0.3 44,280 + SM_b20033 0.76 -0.6
Or see this region's nucleotide sequence