Experiment: Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20020 and SM_b20021 are separated by 116 nucleotides SM_b20021 and SM_b20022 are separated by 647 nucleotides
SM_b20020: SM_b20020 - pyruvate dehydrogenase E1 component, subunits alpha and beta, at 27,345 to 29,423
_b20020
SM_b20021: SM_b20021 - transcriptional regulator, at 29,540 to 30,010
_b20021
SM_b20022: SM_b20022 - hypothetical protein, at 30,658 to 31,224
_b20022
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 28.597 kb on + strand, within SM_b20020 at 28.597 kb on + strand, within SM_b20020 at 28.781 kb on + strand, within SM_b20020 at 28.782 kb on - strand, within SM_b20020 at 28.843 kb on + strand, within SM_b20020 at 28.844 kb on - strand, within SM_b20020 at 28.844 kb on - strand, within SM_b20020 at 28.844 kb on - strand, within SM_b20020 at 28.982 kb on - strand, within SM_b20020 at 29.016 kb on - strand, within SM_b20020 at 29.016 kb on - strand, within SM_b20020 at 29.017 kb on + strand, within SM_b20020 at 29.194 kb on + strand, within SM_b20020 at 29.278 kb on + strand at 29.540 kb on - strand at 29.584 kb on - strand at 29.843 kb on + strand, within SM_b20021 at 29.987 kb on + strand at 29.987 kb on + strand at 29.988 kb on - strand at 30.008 kb on + strand at 30.170 kb on - strand at 30.170 kb on - strand at 30.189 kb on + strand at 30.189 kb on + strand at 30.189 kb on + strand at 30.190 kb on - strand at 30.196 kb on + strand at 30.196 kb on + strand at 30.197 kb on - strand at 30.370 kb on + strand at 30.371 kb on - strand at 30.371 kb on - strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.004 mg/ml remove 28,597 + SM_b20020 0.60 +0.3 28,597 + SM_b20020 0.60 +0.1 28,781 + SM_b20020 0.69 -0.3 28,782 - SM_b20020 0.69 -0.0 28,843 + SM_b20020 0.72 -2.9 28,844 - SM_b20020 0.72 -0.1 28,844 - SM_b20020 0.72 +0.6 28,844 - SM_b20020 0.72 +0.5 28,982 - SM_b20020 0.79 +0.1 29,016 - SM_b20020 0.80 -0.6 29,016 - SM_b20020 0.80 +0.2 29,017 + SM_b20020 0.80 +0.4 29,194 + SM_b20020 0.89 -2.3 29,278 + +0.3 29,540 - +0.6 29,584 - -2.5 29,843 + SM_b20021 0.64 -0.0 29,987 + -0.2 29,987 + -0.3 29,988 - -3.5 30,008 + -1.0 30,170 - -1.6 30,170 - +0.1 30,189 + +0.6 30,189 + +0.1 30,189 + -1.8 30,190 - +0.2 30,196 + -0.4 30,196 + -0.3 30,197 - -0.1 30,370 + +0.8 30,371 - -0.3 30,371 - -0.2 30,471 + -1.0 30,471 + -2.3 30,471 + -0.0 30,471 + -0.7 30,471 + -1.0 30,472 - +0.0
Or see this region's nucleotide sequence