Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_806 and Pf1N1B4_807 are separated by 214 nucleotides Pf1N1B4_807 and Pf1N1B4_808 are separated by 4 nucleotides Pf1N1B4_808 and Pf1N1B4_809 are separated by 568 nucleotides
Pf1N1B4_806 - hypothetical protein, at 922,710 to 924,479
_806
Pf1N1B4_807 - hypothetical protein, at 924,694 to 925,332
_807
Pf1N1B4_808 - hypothetical protein, at 925,337 to 925,477
_808
Pf1N1B4_809 - Adhesin major subunit pilin, at 926,046 to 926,540
_809
Position (kb)
925
926 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 924.358 kb on + strand at 924.358 kb on + strand at 924.359 kb on - strand at 924.359 kb on - strand at 924.359 kb on - strand at 924.390 kb on + strand at 924.390 kb on + strand at 924.390 kb on + strand at 924.390 kb on + strand at 924.390 kb on + strand at 924.391 kb on - strand at 924.542 kb on - strand at 924.671 kb on - strand at 924.697 kb on + strand at 924.698 kb on - strand at 924.734 kb on + strand at 924.734 kb on + strand at 924.734 kb on + strand at 924.735 kb on - strand at 924.764 kb on + strand at 924.764 kb on + strand, within Pf1N1B4_807 at 924.764 kb on + strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.765 kb on - strand, within Pf1N1B4_807 at 924.773 kb on + strand at 924.773 kb on + strand, within Pf1N1B4_807 at 924.773 kb on + strand, within Pf1N1B4_807 at 924.774 kb on - strand, within Pf1N1B4_807 at 924.774 kb on - strand, within Pf1N1B4_807 at 924.799 kb on + strand, within Pf1N1B4_807 at 924.799 kb on + strand, within Pf1N1B4_807 at 924.800 kb on - strand at 924.821 kb on + strand, within Pf1N1B4_807 at 924.822 kb on - strand, within Pf1N1B4_807 at 924.822 kb on - strand, within Pf1N1B4_807 at 924.837 kb on - strand, within Pf1N1B4_807 at 924.865 kb on + strand, within Pf1N1B4_807 at 924.866 kb on - strand, within Pf1N1B4_807 at 924.866 kb on - strand, within Pf1N1B4_807 at 924.983 kb on + strand, within Pf1N1B4_807 at 925.039 kb on - strand, within Pf1N1B4_807 at 925.039 kb on - strand, within Pf1N1B4_807 at 925.039 kb on - strand, within Pf1N1B4_807 at 925.128 kb on + strand at 925.129 kb on - strand, within Pf1N1B4_807 at 925.129 kb on - strand, within Pf1N1B4_807 at 925.129 kb on - strand, within Pf1N1B4_807 at 925.147 kb on - strand, within Pf1N1B4_807 at 925.161 kb on + strand, within Pf1N1B4_807 at 925.162 kb on - strand, within Pf1N1B4_807 at 925.162 kb on - strand, within Pf1N1B4_807 at 925.162 kb on - strand, within Pf1N1B4_807 at 925.162 kb on - strand, within Pf1N1B4_807 at 925.256 kb on + strand, within Pf1N1B4_807 at 925.256 kb on + strand, within Pf1N1B4_807 at 925.273 kb on - strand at 925.319 kb on + strand at 925.341 kb on - strand at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand, within Pf1N1B4_808 at 925.433 kb on + strand at 925.434 kb on - strand, within Pf1N1B4_808 at 925.434 kb on - strand, within Pf1N1B4_808 at 925.434 kb on - strand, within Pf1N1B4_808 at 925.434 kb on - strand, within Pf1N1B4_808 at 925.434 kb on - strand, within Pf1N1B4_808 at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.573 kb on + strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.574 kb on - strand at 925.577 kb on + strand at 925.590 kb on + strand at 925.600 kb on - strand at 925.883 kb on - strand at 925.883 kb on - strand at 925.883 kb on - strand at 925.927 kb on + strand at 926.089 kb on + strand at 926.090 kb on - strand at 926.090 kb on - strand at 926.090 kb on - strand at 926.102 kb on + strand, within Pf1N1B4_809 at 926.102 kb on + strand, within Pf1N1B4_809 at 926.102 kb on + strand, within Pf1N1B4_809 at 926.102 kb on + strand, within Pf1N1B4_809 at 926.102 kb on + strand, within Pf1N1B4_809 at 926.102 kb on + strand, within Pf1N1B4_809 at 926.102 kb on + strand, within Pf1N1B4_809 at 926.103 kb on - strand, within Pf1N1B4_809 at 926.104 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.236 kb on + strand, within Pf1N1B4_809 at 926.237 kb on - strand, within Pf1N1B4_809 at 926.237 kb on - strand, within Pf1N1B4_809 at 926.237 kb on - strand at 926.237 kb on - strand, within Pf1N1B4_809 at 926.267 kb on + strand, within Pf1N1B4_809 at 926.267 kb on + strand, within Pf1N1B4_809 at 926.268 kb on - strand, within Pf1N1B4_809 at 926.268 kb on - strand, within Pf1N1B4_809 at 926.269 kb on + strand, within Pf1N1B4_809 at 926.269 kb on + strand, within Pf1N1B4_809 at 926.270 kb on - strand, within Pf1N1B4_809 at 926.270 kb on - strand, within Pf1N1B4_809 at 926.270 kb on - strand, within Pf1N1B4_809 at 926.326 kb on + strand at 926.326 kb on + strand, within Pf1N1B4_809 at 926.326 kb on + strand, within Pf1N1B4_809 at 926.326 kb on + strand, within Pf1N1B4_809 at 926.327 kb on - strand, within Pf1N1B4_809 at 926.354 kb on + strand, within Pf1N1B4_809 at 926.354 kb on + strand, within Pf1N1B4_809 at 926.355 kb on - strand, within Pf1N1B4_809 at 926.416 kb on + strand, within Pf1N1B4_809 at 926.416 kb on + strand, within Pf1N1B4_809
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 924,358 + +2.6 924,358 + +1.0 924,359 - +2.0 924,359 - +0.6 924,359 - -1.5 924,390 + -1.3 924,390 + -0.3 924,390 + -1.5 924,390 + +0.3 924,390 + +1.0 924,391 - +0.0 924,542 - -1.0 924,671 - +1.0 924,697 + +0.7 924,698 - -0.2 924,734 + +0.3 924,734 + -0.6 924,734 + +0.0 924,735 - -3.1 924,764 + +0.0 924,764 + Pf1N1B4_807 0.11 -0.1 924,764 + Pf1N1B4_807 0.11 +1.0 924,765 - Pf1N1B4_807 0.11 -1.0 924,765 - Pf1N1B4_807 0.11 +0.3 924,765 - Pf1N1B4_807 0.11 -1.3 924,765 - Pf1N1B4_807 0.11 +2.0 924,765 - Pf1N1B4_807 0.11 -1.3 924,765 - Pf1N1B4_807 0.11 -1.9 924,765 - Pf1N1B4_807 0.11 +0.6 924,765 - Pf1N1B4_807 0.11 +0.5 924,773 + +0.0 924,773 + Pf1N1B4_807 0.12 +1.0 924,773 + Pf1N1B4_807 0.12 -0.5 924,774 - Pf1N1B4_807 0.13 -1.5 924,774 - Pf1N1B4_807 0.13 -0.5 924,799 + Pf1N1B4_807 0.16 -1.5 924,799 + Pf1N1B4_807 0.16 -1.8 924,800 - +0.0 924,821 + Pf1N1B4_807 0.20 +0.6 924,822 - Pf1N1B4_807 0.20 -0.5 924,822 - Pf1N1B4_807 0.20 +0.0 924,837 - Pf1N1B4_807 0.22 -0.4 924,865 + Pf1N1B4_807 0.27 +1.6 924,866 - Pf1N1B4_807 0.27 -0.4 924,866 - Pf1N1B4_807 0.27 +0.3 924,983 + Pf1N1B4_807 0.45 -0.5 925,039 - Pf1N1B4_807 0.54 +1.0 925,039 - Pf1N1B4_807 0.54 -0.8 925,039 - Pf1N1B4_807 0.54 -0.5 925,128 + +0.0 925,129 - Pf1N1B4_807 0.68 +0.0 925,129 - Pf1N1B4_807 0.68 -0.1 925,129 - Pf1N1B4_807 0.68 +0.6 925,147 - Pf1N1B4_807 0.71 +0.2 925,161 + Pf1N1B4_807 0.73 -1.0 925,162 - Pf1N1B4_807 0.73 -0.5 925,162 - Pf1N1B4_807 0.73 -1.5 925,162 - Pf1N1B4_807 0.73 -0.5 925,162 - Pf1N1B4_807 0.73 +2.0 925,256 + Pf1N1B4_807 0.88 +0.0 925,256 + Pf1N1B4_807 0.88 +0.3 925,273 - -0.1 925,319 + -0.1 925,341 - -0.9 925,433 + Pf1N1B4_808 0.68 -0.7 925,433 + Pf1N1B4_808 0.68 -1.7 925,433 + Pf1N1B4_808 0.68 -1.5 925,433 + Pf1N1B4_808 0.68 -1.3 925,433 + Pf1N1B4_808 0.68 -1.5 925,433 + Pf1N1B4_808 0.68 -0.6 925,433 + Pf1N1B4_808 0.68 -2.0 925,433 + Pf1N1B4_808 0.68 -1.8 925,433 + Pf1N1B4_808 0.68 -1.0 925,433 + Pf1N1B4_808 0.68 +0.5 925,433 + Pf1N1B4_808 0.68 -0.3 925,433 + -1.5 925,434 - Pf1N1B4_808 0.69 +1.0 925,434 - Pf1N1B4_808 0.69 -0.2 925,434 - Pf1N1B4_808 0.69 -1.3 925,434 - Pf1N1B4_808 0.69 -2.3 925,434 - Pf1N1B4_808 0.69 +2.0 925,573 + +0.4 925,573 + -1.3 925,573 + -2.7 925,573 + -0.5 925,573 + -1.1 925,573 + -1.5 925,573 + +1.0 925,573 + -1.3 925,573 + -0.1 925,573 + -0.3 925,573 + -1.3 925,573 + -0.2 925,573 + -2.3 925,573 + +0.5 925,574 - -0.5 925,574 - +0.0 925,574 - -0.3 925,574 - +0.0 925,574 - +0.5 925,574 - -0.4 925,574 - +1.0 925,574 - -1.1 925,574 - +0.4 925,574 - -0.8 925,577 + -0.4 925,590 + -0.8 925,600 - -1.5 925,883 - -1.5 925,883 - -0.5 925,883 - -0.5 925,927 + +0.0 926,089 + +0.5 926,090 - +0.5 926,090 - -2.3 926,090 - +0.0 926,102 + Pf1N1B4_809 0.11 -0.2 926,102 + Pf1N1B4_809 0.11 -0.4 926,102 + Pf1N1B4_809 0.11 +0.5 926,102 + Pf1N1B4_809 0.11 -0.5 926,102 + Pf1N1B4_809 0.11 -0.1 926,102 + Pf1N1B4_809 0.11 -1.5 926,102 + Pf1N1B4_809 0.11 +1.0 926,103 - Pf1N1B4_809 0.12 +1.0 926,104 + Pf1N1B4_809 0.12 -0.8 926,236 + Pf1N1B4_809 0.38 -0.2 926,236 + Pf1N1B4_809 0.38 +1.0 926,236 + Pf1N1B4_809 0.38 +1.2 926,236 + Pf1N1B4_809 0.38 -0.7 926,236 + Pf1N1B4_809 0.38 -0.9 926,236 + Pf1N1B4_809 0.38 -1.0 926,236 + Pf1N1B4_809 0.38 -0.3 926,236 + Pf1N1B4_809 0.38 +1.4 926,236 + Pf1N1B4_809 0.38 +0.9 926,237 - Pf1N1B4_809 0.39 -1.1 926,237 - Pf1N1B4_809 0.39 +1.4 926,237 - -0.5 926,237 - Pf1N1B4_809 0.39 -0.4 926,267 + Pf1N1B4_809 0.45 -0.1 926,267 + Pf1N1B4_809 0.45 -0.5 926,268 - Pf1N1B4_809 0.45 +0.2 926,268 - Pf1N1B4_809 0.45 +1.0 926,269 + Pf1N1B4_809 0.45 -1.8 926,269 + Pf1N1B4_809 0.45 +0.5 926,270 - Pf1N1B4_809 0.45 +0.3 926,270 - Pf1N1B4_809 0.45 -0.5 926,270 - Pf1N1B4_809 0.45 -0.5 926,326 + +0.5 926,326 + Pf1N1B4_809 0.57 -1.5 926,326 + Pf1N1B4_809 0.57 +0.3 926,326 + Pf1N1B4_809 0.57 -1.5 926,327 - Pf1N1B4_809 0.57 +0.5 926,354 + Pf1N1B4_809 0.62 -1.0 926,354 + Pf1N1B4_809 0.62 +1.6 926,355 - Pf1N1B4_809 0.62 -2.0 926,416 + Pf1N1B4_809 0.75 -2.0 926,416 + Pf1N1B4_809 0.75 +0.0
Or see this region's nucleotide sequence