Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5897

Experiment: Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5896 and Pf1N1B4_5897 overlap by 4 nucleotidesPf1N1B4_5897 and Pf1N1B4_5898 are separated by 173 nucleotidesPf1N1B4_5898 and Pf1N1B4_5899 are separated by 4 nucleotides Pf1N1B4_5896 - Polysaccharide biosynthesis protein, at 339,330 to 340,634 _5896 Pf1N1B4_5897 - FIG00963512: hypothetical protein, at 340,631 to 342,550 _5897 Pf1N1B4_5898 - hypothetical protein, at 342,724 to 343,332 _5898 Pf1N1B4_5899 - FIG00954746: hypothetical protein, at 343,337 to 344,551 _5899 Position (kb) 340 341 342 343Strain fitness (log2 ratio) -2 -1 0 1at 340.202 kb on - strand, within Pf1N1B4_5896at 340.291 kb on - strandat 340.536 kb on + strandat 340.536 kb on + strandat 340.536 kb on + strandat 340.537 kb on - strandat 340.537 kb on - strandat 340.537 kb on - strandat 340.705 kb on + strandat 340.757 kb on - strandat 340.929 kb on - strand, within Pf1N1B4_5897at 341.033 kb on + strand, within Pf1N1B4_5897at 341.033 kb on + strand, within Pf1N1B4_5897at 341.034 kb on - strand, within Pf1N1B4_5897at 341.382 kb on - strand, within Pf1N1B4_5897at 341.382 kb on - strand, within Pf1N1B4_5897at 341.382 kb on - strand, within Pf1N1B4_5897at 341.391 kb on - strand, within Pf1N1B4_5897at 341.456 kb on + strand, within Pf1N1B4_5897at 341.517 kb on - strand, within Pf1N1B4_5897at 341.664 kb on - strand, within Pf1N1B4_5897at 341.696 kb on + strand, within Pf1N1B4_5897at 341.697 kb on - strand, within Pf1N1B4_5897at 341.705 kb on + strand, within Pf1N1B4_5897at 341.748 kb on + strand, within Pf1N1B4_5897at 342.358 kb on - strand, within Pf1N1B4_5897at 342.368 kb on - strandat 342.437 kb on + strandat 342.438 kb on - strandat 342.643 kb on - strandat 342.643 kb on - strandat 342.721 kb on + strandat 342.721 kb on + strandat 342.905 kb on - strand, within Pf1N1B4_5898at 342.919 kb on + strand, within Pf1N1B4_5898at 342.920 kb on - strand, within Pf1N1B4_5898at 342.991 kb on + strandat 343.158 kb on + strand, within Pf1N1B4_5898at 343.158 kb on + strand, within Pf1N1B4_5898at 343.159 kb on - strand, within Pf1N1B4_5898at 343.161 kb on - strand, within Pf1N1B4_5898at 343.430 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 200 mM
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340,202 - Pf1N1B4_5896 0.67 -0.1
340,291 - +0.5
340,536 + +0.5
340,536 + -0.7
340,536 + -2.3
340,537 - -0.7
340,537 - -0.4
340,537 - -0.6
340,705 + -0.3
340,757 - +1.3
340,929 - Pf1N1B4_5897 0.16 -0.7
341,033 + Pf1N1B4_5897 0.21 -0.8
341,033 + Pf1N1B4_5897 0.21 -1.6
341,034 - Pf1N1B4_5897 0.21 -0.1
341,382 - Pf1N1B4_5897 0.39 -0.5
341,382 - Pf1N1B4_5897 0.39 -0.5
341,382 - Pf1N1B4_5897 0.39 -1.5
341,391 - Pf1N1B4_5897 0.40 -0.5
341,456 + Pf1N1B4_5897 0.43 -0.2
341,517 - Pf1N1B4_5897 0.46 -0.5
341,664 - Pf1N1B4_5897 0.54 -0.7
341,696 + Pf1N1B4_5897 0.55 -0.1
341,697 - Pf1N1B4_5897 0.56 +0.8
341,705 + Pf1N1B4_5897 0.56 -2.4
341,748 + Pf1N1B4_5897 0.58 -1.1
342,358 - Pf1N1B4_5897 0.90 -0.5
342,368 - -0.5
342,437 + -1.1
342,438 - -0.3
342,643 - -0.2
342,643 - -0.1
342,721 + -0.3
342,721 + -0.3
342,905 - Pf1N1B4_5898 0.30 +0.5
342,919 + Pf1N1B4_5898 0.32 -0.2
342,920 - Pf1N1B4_5898 0.32 -0.6
342,991 + -1.5
343,158 + Pf1N1B4_5898 0.71 -0.1
343,158 + Pf1N1B4_5898 0.71 -0.8
343,159 - Pf1N1B4_5898 0.71 -0.5
343,161 - Pf1N1B4_5898 0.72 -0.2
343,430 - -0.9

Or see this region's nucleotide sequence