Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5023

Experiment: Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5021 and Pf1N1B4_5022 are separated by 127 nucleotidesPf1N1B4_5022 and Pf1N1B4_5023 overlap by 11 nucleotidesPf1N1B4_5023 and Pf1N1B4_5024 are separated by 60 nucleotides Pf1N1B4_5021 - FIG00958580: hypothetical protein, at 5,569,700 to 5,569,876 _5021 Pf1N1B4_5022 - hypothetical protein, at 5,570,004 to 5,570,714 _5022 Pf1N1B4_5023 - Alkylated DNA repair protein AlkB, at 5,570,704 to 5,571,384 _5023 Pf1N1B4_5024 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,571,445 to 5,572,545 _5024 Position (kb) 5570 5571 5572Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5569.725 kb on - strandat 5569.725 kb on - strand, within Pf1N1B4_5021at 5570.009 kb on + strandat 5570.122 kb on + strandat 5570.122 kb on + strand, within Pf1N1B4_5022at 5570.134 kb on - strand, within Pf1N1B4_5022at 5570.199 kb on + strand, within Pf1N1B4_5022at 5570.199 kb on + strand, within Pf1N1B4_5022at 5570.200 kb on - strand, within Pf1N1B4_5022at 5570.200 kb on - strand, within Pf1N1B4_5022at 5570.206 kb on - strandat 5570.206 kb on - strand, within Pf1N1B4_5022at 5570.289 kb on + strand, within Pf1N1B4_5022at 5570.289 kb on + strandat 5570.394 kb on + strand, within Pf1N1B4_5022at 5570.409 kb on + strand, within Pf1N1B4_5022at 5570.410 kb on - strand, within Pf1N1B4_5022at 5570.800 kb on + strand, within Pf1N1B4_5023at 5570.801 kb on - strand, within Pf1N1B4_5023at 5570.801 kb on - strand, within Pf1N1B4_5023at 5570.801 kb on - strand, within Pf1N1B4_5023at 5570.907 kb on + strand, within Pf1N1B4_5023at 5570.908 kb on - strandat 5570.908 kb on - strand, within Pf1N1B4_5023at 5570.974 kb on + strand, within Pf1N1B4_5023at 5571.108 kb on + strand, within Pf1N1B4_5023at 5571.109 kb on - strand, within Pf1N1B4_5023at 5571.109 kb on - strandat 5571.109 kb on - strand, within Pf1N1B4_5023at 5571.109 kb on - strand, within Pf1N1B4_5023at 5571.418 kb on - strandat 5571.418 kb on - strandat 5571.437 kb on + strandat 5571.566 kb on - strand, within Pf1N1B4_5024at 5571.566 kb on - strand, within Pf1N1B4_5024at 5571.566 kb on - strand, within Pf1N1B4_5024at 5571.566 kb on - strand, within Pf1N1B4_5024at 5571.589 kb on + strand, within Pf1N1B4_5024at 5571.590 kb on - strandat 5571.590 kb on - strand, within Pf1N1B4_5024at 5571.946 kb on + strand, within Pf1N1B4_5024at 5571.946 kb on + strand, within Pf1N1B4_5024at 5571.946 kb on + strand, within Pf1N1B4_5024at 5571.946 kb on + strand, within Pf1N1B4_5024at 5571.946 kb on + strand, within Pf1N1B4_5024at 5571.947 kb on - strand, within Pf1N1B4_5024at 5571.979 kb on + strand, within Pf1N1B4_5024at 5571.979 kb on + strand, within Pf1N1B4_5024at 5571.980 kb on - strand, within Pf1N1B4_5024at 5572.280 kb on + strand, within Pf1N1B4_5024at 5572.351 kb on + strand, within Pf1N1B4_5024at 5572.361 kb on + strand, within Pf1N1B4_5024at 5572.361 kb on + strand, within Pf1N1B4_5024at 5572.361 kb on + strand, within Pf1N1B4_5024at 5572.362 kb on - strand, within Pf1N1B4_5024

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 200 mM
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5,569,725 - -0.6
5,569,725 - Pf1N1B4_5021 0.14 +0.8
5,570,009 + +1.4
5,570,122 + +0.4
5,570,122 + Pf1N1B4_5022 0.17 -0.1
5,570,134 - Pf1N1B4_5022 0.18 -3.1
5,570,199 + Pf1N1B4_5022 0.27 -0.2
5,570,199 + Pf1N1B4_5022 0.27 -0.4
5,570,200 - Pf1N1B4_5022 0.28 +0.4
5,570,200 - Pf1N1B4_5022 0.28 -2.2
5,570,206 - -1.2
5,570,206 - Pf1N1B4_5022 0.28 -0.1
5,570,289 + Pf1N1B4_5022 0.40 +0.6
5,570,289 + -0.0
5,570,394 + Pf1N1B4_5022 0.55 +0.7
5,570,409 + Pf1N1B4_5022 0.57 -0.6
5,570,410 - Pf1N1B4_5022 0.57 -0.9
5,570,800 + Pf1N1B4_5023 0.14 +1.4
5,570,801 - Pf1N1B4_5023 0.14 -2.2
5,570,801 - Pf1N1B4_5023 0.14 -0.0
5,570,801 - Pf1N1B4_5023 0.14 -1.2
5,570,907 + Pf1N1B4_5023 0.30 -3.4
5,570,908 - +0.4
5,570,908 - Pf1N1B4_5023 0.30 +0.4
5,570,974 + Pf1N1B4_5023 0.40 -1.6
5,571,108 + Pf1N1B4_5023 0.59 -1.2
5,571,109 - Pf1N1B4_5023 0.59 -0.2
5,571,109 - +1.0
5,571,109 - Pf1N1B4_5023 0.59 +0.2
5,571,109 - Pf1N1B4_5023 0.59 -2.2
5,571,418 - +0.8
5,571,418 - -0.0
5,571,437 + -0.0
5,571,566 - Pf1N1B4_5024 0.11 -0.3
5,571,566 - Pf1N1B4_5024 0.11 +0.9
5,571,566 - Pf1N1B4_5024 0.11 -0.6
5,571,566 - Pf1N1B4_5024 0.11 -0.8
5,571,589 + Pf1N1B4_5024 0.13 +0.4
5,571,590 - +1.4
5,571,590 - Pf1N1B4_5024 0.13 -0.0
5,571,946 + Pf1N1B4_5024 0.46 -0.9
5,571,946 + Pf1N1B4_5024 0.46 +0.1
5,571,946 + Pf1N1B4_5024 0.46 -1.2
5,571,946 + Pf1N1B4_5024 0.46 -1.1
5,571,946 + Pf1N1B4_5024 0.46 -0.0
5,571,947 - Pf1N1B4_5024 0.46 -0.7
5,571,979 + Pf1N1B4_5024 0.49 +0.1
5,571,979 + Pf1N1B4_5024 0.49 -1.9
5,571,980 - Pf1N1B4_5024 0.49 +0.0
5,572,280 + Pf1N1B4_5024 0.76 +3.4
5,572,351 + Pf1N1B4_5024 0.82 +0.4
5,572,361 + Pf1N1B4_5024 0.83 +0.6
5,572,361 + Pf1N1B4_5024 0.83 +0.6
5,572,361 + Pf1N1B4_5024 0.83 -0.6
5,572,362 - Pf1N1B4_5024 0.83 +0.2

Or see this region's nucleotide sequence