Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_321

Experiment: Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_320 and Pf1N1B4_321 are separated by 32 nucleotidesPf1N1B4_321 and Pf1N1B4_322 are separated by 206 nucleotides Pf1N1B4_320 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 376,354 to 377,628 _320 Pf1N1B4_321 - Probable short-chain dehydrogenase, at 377,661 to 378,455 _321 Pf1N1B4_322 - nucleotide sugar epimerase/dehydratase WbpM, at 378,662 to 380,890 _322 Position (kb) 377 378 379Strain fitness (log2 ratio) -2 -1 0 1 2at 376.797 kb on - strand, within Pf1N1B4_320at 376.797 kb on - strand, within Pf1N1B4_320at 376.797 kb on - strandat 376.965 kb on - strandat 377.049 kb on + strand, within Pf1N1B4_320at 377.049 kb on + strand, within Pf1N1B4_320at 377.050 kb on - strand, within Pf1N1B4_320at 377.050 kb on - strand, within Pf1N1B4_320at 377.379 kb on + strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.638 kb on - strandat 377.638 kb on - strandat 377.638 kb on - strandat 377.669 kb on - strandat 377.691 kb on - strandat 377.691 kb on - strandat 377.691 kb on - strandat 377.726 kb on + strandat 377.726 kb on + strandat 377.727 kb on - strandat 377.885 kb on + strand, within Pf1N1B4_321at 377.886 kb on - strand, within Pf1N1B4_321at 377.971 kb on + strandat 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strandat 377.989 kb on + strand, within Pf1N1B4_321at 377.990 kb on - strand, within Pf1N1B4_321at 377.990 kb on - strand, within Pf1N1B4_321at 377.990 kb on - strand, within Pf1N1B4_321at 378.039 kb on - strand, within Pf1N1B4_321at 378.186 kb on - strand, within Pf1N1B4_321at 378.539 kb on + strandat 378.540 kb on - strandat 378.540 kb on - strandat 378.553 kb on + strandat 378.554 kb on - strandat 378.554 kb on - strandat 378.558 kb on + strandat 378.565 kb on - strandat 378.613 kb on - strandat 378.613 kb on - strandat 378.656 kb on - strandat 378.656 kb on - strandat 378.656 kb on - strandat 378.664 kb on - strandat 378.664 kb on - strandat 378.664 kb on - strandat 378.664 kb on - strandat 378.664 kb on - strandat 378.664 kb on - strandat 378.664 kb on - strandat 378.664 kb on - strandat 378.700 kb on + strandat 378.701 kb on - strandat 378.944 kb on - strand, within Pf1N1B4_322at 378.979 kb on - strand, within Pf1N1B4_322at 378.979 kb on - strandat 378.979 kb on - strand, within Pf1N1B4_322at 378.998 kb on - strand, within Pf1N1B4_322at 378.998 kb on - strand, within Pf1N1B4_322

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 200 mM
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376,797 - Pf1N1B4_320 0.35 +0.6
376,797 - Pf1N1B4_320 0.35 +0.1
376,797 - +1.4
376,965 - +0.4
377,049 + Pf1N1B4_320 0.55 +0.8
377,049 + Pf1N1B4_320 0.55 -0.2
377,050 - Pf1N1B4_320 0.55 -1.8
377,050 - Pf1N1B4_320 0.55 -0.5
377,379 + Pf1N1B4_320 0.80 -0.8
377,380 - Pf1N1B4_320 0.80 +1.0
377,380 - Pf1N1B4_320 0.80 -0.2
377,380 - Pf1N1B4_320 0.80 -0.2
377,380 - Pf1N1B4_320 0.80 +0.0
377,638 - +1.8
377,638 - -0.6
377,638 - -1.0
377,669 - -0.1
377,691 - -1.2
377,691 - -1.8
377,691 - -0.3
377,726 + +0.8
377,726 + +1.4
377,727 - -0.4
377,885 + Pf1N1B4_321 0.28 +0.6
377,886 - Pf1N1B4_321 0.28 -0.3
377,971 + +0.2
377,972 - Pf1N1B4_321 0.39 +0.6
377,972 - Pf1N1B4_321 0.39 -0.4
377,972 - Pf1N1B4_321 0.39 +0.3
377,972 - Pf1N1B4_321 0.39 -1.1
377,972 - Pf1N1B4_321 0.39 +1.7
377,972 - Pf1N1B4_321 0.39 +0.6
377,972 - Pf1N1B4_321 0.39 +0.0
377,972 - Pf1N1B4_321 0.39 -0.0
377,972 - Pf1N1B4_321 0.39 -0.8
377,972 - Pf1N1B4_321 0.39 +1.5
377,972 - Pf1N1B4_321 0.39 -0.2
377,972 - Pf1N1B4_321 0.39 +0.1
377,972 - Pf1N1B4_321 0.39 -0.7
377,972 - -0.8
377,989 + Pf1N1B4_321 0.41 -0.2
377,990 - Pf1N1B4_321 0.41 -0.7
377,990 - Pf1N1B4_321 0.41 +0.1
377,990 - Pf1N1B4_321 0.41 +0.0
378,039 - Pf1N1B4_321 0.48 -0.2
378,186 - Pf1N1B4_321 0.66 -0.4
378,539 + -1.8
378,540 - +1.6
378,540 - +0.4
378,553 + -0.2
378,554 - +0.8
378,554 - +0.3
378,558 + -0.6
378,565 - -0.0
378,613 - +0.4
378,613 - -1.5
378,656 - -0.2
378,656 - -1.5
378,656 - -0.2
378,664 - +0.1
378,664 - -1.5
378,664 - +0.0
378,664 - -1.2
378,664 - -0.4
378,664 - -0.8
378,664 - -1.9
378,664 - -1.2
378,700 + +0.6
378,701 - -0.2
378,944 - Pf1N1B4_322 0.13 +1.4
378,979 - Pf1N1B4_322 0.14 +0.6
378,979 - +0.2
378,979 - Pf1N1B4_322 0.14 +1.2
378,998 - Pf1N1B4_322 0.15 -1.6
378,998 - Pf1N1B4_322 0.15 -0.6

Or see this region's nucleotide sequence