Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_24 and Pf1N1B4_25 overlap by 30 nucleotides Pf1N1B4_25 and Pf1N1B4_26 are separated by 257 nucleotides Pf1N1B4_26 and Pf1N1B4_27 are separated by 337 nucleotides
Pf1N1B4_24 - Glutamyl-tRNA synthetase (EC 6.1.1.17), at 26,536 to 27,849
_24
Pf1N1B4_25 - hypothetical protein, at 27,820 to 27,942
_25
Pf1N1B4_26 - Excinuclease ABC subunit B, at 28,200 to 30,215
_26
Pf1N1B4_27 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 30,553 to 31,749
_27
Position (kb)
28
29
30
31 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 27.941 kb on - strand at 28.007 kb on + strand at 28.007 kb on + strand at 28.007 kb on + strand at 28.011 kb on + strand at 28.012 kb on - strand at 28.029 kb on - strand at 28.064 kb on + strand at 28.064 kb on + strand at 28.064 kb on + strand at 28.122 kb on - strand at 28.355 kb on - strand at 29.023 kb on + strand, within Pf1N1B4_26 at 29.024 kb on - strand, within Pf1N1B4_26 at 29.166 kb on - strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.168 kb on - strand, within Pf1N1B4_26 at 29.168 kb on - strand at 29.168 kb on - strand, within Pf1N1B4_26 at 29.168 kb on - strand, within Pf1N1B4_26 at 29.236 kb on + strand, within Pf1N1B4_26 at 29.401 kb on + strand, within Pf1N1B4_26 at 29.488 kb on + strand, within Pf1N1B4_26 at 29.616 kb on - strand at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.678 kb on - strand, within Pf1N1B4_26 at 29.758 kb on + strand, within Pf1N1B4_26 at 29.759 kb on - strand, within Pf1N1B4_26 at 29.782 kb on + strand, within Pf1N1B4_26 at 29.918 kb on - strand, within Pf1N1B4_26 at 29.936 kb on - strand, within Pf1N1B4_26 at 29.944 kb on + strand, within Pf1N1B4_26 at 29.945 kb on - strand, within Pf1N1B4_26 at 29.957 kb on - strand, within Pf1N1B4_26 at 29.998 kb on + strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 30.198 kb on + strand at 30.215 kb on + strand at 30.215 kb on + strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.355 kb on - strand at 30.464 kb on + strand at 30.465 kb on - strand at 30.466 kb on + strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.470 kb on - strand at 30.494 kb on - strand at 30.551 kb on + strand at 30.664 kb on + strand at 30.778 kb on + strand, within Pf1N1B4_27 at 31.006 kb on + strand, within Pf1N1B4_27 at 31.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 27,941 - -0.0 28,007 + +2.3 28,007 + +0.3 28,007 + -0.9 28,011 + -0.9 28,012 - -0.2 28,029 - +0.4 28,064 + -0.3 28,064 + -0.3 28,064 + -1.3 28,122 - -0.3 28,355 - +0.7 29,023 + Pf1N1B4_26 0.41 -1.4 29,024 - Pf1N1B4_26 0.41 -1.4 29,166 - Pf1N1B4_26 0.48 -2.2 29,167 + Pf1N1B4_26 0.48 -0.9 29,167 + Pf1N1B4_26 0.48 -3.2 29,167 + Pf1N1B4_26 0.48 +1.5 29,167 + Pf1N1B4_26 0.48 -0.6 29,167 + Pf1N1B4_26 0.48 -1.6 29,168 - Pf1N1B4_26 0.48 -0.7 29,168 - -0.3 29,168 - Pf1N1B4_26 0.48 -0.0 29,168 - Pf1N1B4_26 0.48 -1.6 29,236 + Pf1N1B4_26 0.51 -0.5 29,401 + Pf1N1B4_26 0.60 -0.3 29,488 + Pf1N1B4_26 0.64 -1.3 29,616 - +0.7 29,677 + Pf1N1B4_26 0.73 -0.3 29,677 + Pf1N1B4_26 0.73 -0.3 29,677 + Pf1N1B4_26 0.73 -1.9 29,678 - Pf1N1B4_26 0.73 -0.0 29,758 + Pf1N1B4_26 0.77 -0.9 29,759 - Pf1N1B4_26 0.77 -0.8 29,782 + Pf1N1B4_26 0.78 -0.5 29,918 - Pf1N1B4_26 0.85 -1.4 29,936 - Pf1N1B4_26 0.86 -0.1 29,944 + Pf1N1B4_26 0.87 -0.3 29,945 - Pf1N1B4_26 0.87 +0.1 29,957 - Pf1N1B4_26 0.87 +0.7 29,998 + Pf1N1B4_26 0.89 -0.9 29,999 - Pf1N1B4_26 0.89 -0.9 29,999 - Pf1N1B4_26 0.89 -0.7 30,198 + -0.3 30,215 + -0.7 30,215 + +1.1 30,216 - -1.9 30,216 - -0.4 30,216 - -0.3 30,216 - +1.9 30,216 - +1.3 30,216 - -0.0 30,216 - -0.2 30,216 - +0.7 30,216 - -0.3 30,219 + +0.1 30,219 + -1.3 30,219 + -0.7 30,220 - +0.6 30,220 - +0.7 30,220 - -0.5 30,220 - +0.1 30,220 - +0.1 30,220 - +0.3 30,220 - -1.1 30,220 - -0.7 30,220 - +1.3 30,355 - -0.7 30,464 + -0.6 30,465 - +0.6 30,466 + -1.6 30,467 - -0.6 30,467 - -1.6 30,467 - -1.4 30,467 - +0.4 30,469 + +0.7 30,469 + +0.7 30,469 + +0.3 30,469 + +0.7 30,469 + -0.9 30,469 + +2.0 30,469 + -0.2 30,470 - -0.3 30,494 - +1.5 30,551 + -0.3 30,664 + -0.5 30,778 + Pf1N1B4_27 0.19 -0.4 31,006 + Pf1N1B4_27 0.38 -0.0 31,007 - -1.3
Or see this region's nucleotide sequence