Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1864 and Pf1N1B4_1865 are separated by 60 nucleotides Pf1N1B4_1865 and Pf1N1B4_1866 overlap by 1 nucleotides
Pf1N1B4_1864 - Methionine ABC transporter substrate-binding protein, at 2,133,652 to 2,134,425
_1864
Pf1N1B4_1865 - Methionine ABC transporter permease protein, at 2,134,486 to 2,135,160
_1865
Pf1N1B4_1866 - Methionine ABC transporter ATP-binding protein, at 2,135,160 to 2,136,167
_1866
Position (kb)
2134
2135
2136 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2133.504 kb on + strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.505 kb on - strand at 2133.539 kb on + strand at 2133.540 kb on - strand at 2133.540 kb on - strand at 2133.540 kb on - strand at 2133.544 kb on + strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.545 kb on - strand at 2133.555 kb on + strand at 2133.556 kb on - strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.680 kb on + strand at 2133.681 kb on - strand at 2133.681 kb on - strand at 2133.788 kb on + strand, within Pf1N1B4_1864 at 2133.788 kb on + strand, within Pf1N1B4_1864 at 2133.789 kb on - strand, within Pf1N1B4_1864 at 2133.789 kb on - strand, within Pf1N1B4_1864 at 2133.854 kb on + strand, within Pf1N1B4_1864 at 2133.854 kb on + strand, within Pf1N1B4_1864 at 2133.855 kb on - strand, within Pf1N1B4_1864 at 2133.855 kb on - strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.100 kb on + strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.101 kb on - strand, within Pf1N1B4_1864 at 2134.112 kb on + strand, within Pf1N1B4_1864 at 2134.112 kb on + strand, within Pf1N1B4_1864 at 2134.113 kb on - strand, within Pf1N1B4_1864 at 2134.191 kb on - strand, within Pf1N1B4_1864 at 2134.256 kb on + strand, within Pf1N1B4_1864 at 2134.256 kb on + strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.257 kb on - strand, within Pf1N1B4_1864 at 2134.414 kb on + strand at 2134.432 kb on - strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.487 kb on + strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.488 kb on - strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.491 kb on + strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.492 kb on - strand at 2134.661 kb on + strand, within Pf1N1B4_1865 at 2134.662 kb on - strand, within Pf1N1B4_1865 at 2134.662 kb on - strand, within Pf1N1B4_1865 at 2134.674 kb on + strand, within Pf1N1B4_1865 at 2134.674 kb on + strand, within Pf1N1B4_1865 at 2134.675 kb on - strand, within Pf1N1B4_1865 at 2134.675 kb on - strand, within Pf1N1B4_1865 at 2134.675 kb on - strand at 2134.689 kb on + strand, within Pf1N1B4_1865 at 2134.689 kb on + strand, within Pf1N1B4_1865 at 2134.690 kb on - strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.932 kb on + strand, within Pf1N1B4_1865 at 2134.933 kb on - strand, within Pf1N1B4_1865 at 2134.933 kb on - strand, within Pf1N1B4_1865 at 2134.933 kb on - strand, within Pf1N1B4_1865 at 2134.982 kb on + strand, within Pf1N1B4_1865 at 2134.983 kb on - strand, within Pf1N1B4_1865 at 2135.065 kb on + strand, within Pf1N1B4_1865 at 2135.065 kb on + strand, within Pf1N1B4_1865 at 2135.066 kb on - strand, within Pf1N1B4_1865 at 2135.095 kb on - strand at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.299 kb on + strand, within Pf1N1B4_1866 at 2135.300 kb on - strand, within Pf1N1B4_1866 at 2135.344 kb on + strand, within Pf1N1B4_1866 at 2136.032 kb on + strand, within Pf1N1B4_1866 at 2136.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 2,133,504 + -0.1 2,133,505 - +0.4 2,133,505 - -0.1 2,133,505 - -1.7 2,133,505 - +0.5 2,133,505 - +2.5 2,133,539 + -0.1 2,133,540 - +0.5 2,133,540 - -0.6 2,133,540 - +1.3 2,133,544 + -0.5 2,133,545 - +0.9 2,133,545 - -0.1 2,133,545 - -1.9 2,133,545 - -0.1 2,133,545 - -1.7 2,133,555 + +0.2 2,133,556 - +1.1 2,133,680 + +0.9 2,133,680 + +0.1 2,133,680 + -0.1 2,133,680 + -0.1 2,133,680 + +0.5 2,133,680 + +0.2 2,133,680 + +0.3 2,133,680 + -1.1 2,133,681 - +0.5 2,133,681 - -0.8 2,133,788 + Pf1N1B4_1864 0.18 -0.5 2,133,788 + Pf1N1B4_1864 0.18 -0.9 2,133,789 - Pf1N1B4_1864 0.18 -0.5 2,133,789 - Pf1N1B4_1864 0.18 -0.3 2,133,854 + Pf1N1B4_1864 0.26 -0.7 2,133,854 + Pf1N1B4_1864 0.26 +1.5 2,133,855 - Pf1N1B4_1864 0.26 -0.7 2,133,855 - Pf1N1B4_1864 0.26 -0.1 2,134,100 + Pf1N1B4_1864 0.58 -1.3 2,134,100 + Pf1N1B4_1864 0.58 -0.1 2,134,100 + Pf1N1B4_1864 0.58 -1.5 2,134,100 + Pf1N1B4_1864 0.58 -1.1 2,134,100 + Pf1N1B4_1864 0.58 +1.2 2,134,100 + Pf1N1B4_1864 0.58 +1.2 2,134,101 - Pf1N1B4_1864 0.58 +1.8 2,134,101 - Pf1N1B4_1864 0.58 -0.4 2,134,101 - Pf1N1B4_1864 0.58 +1.2 2,134,101 - Pf1N1B4_1864 0.58 -0.9 2,134,101 - Pf1N1B4_1864 0.58 +0.9 2,134,101 - Pf1N1B4_1864 0.58 +0.7 2,134,101 - Pf1N1B4_1864 0.58 -0.7 2,134,101 - Pf1N1B4_1864 0.58 +0.5 2,134,101 - Pf1N1B4_1864 0.58 -0.7 2,134,112 + Pf1N1B4_1864 0.59 +0.3 2,134,112 + Pf1N1B4_1864 0.59 -0.1 2,134,113 - Pf1N1B4_1864 0.60 -1.3 2,134,191 - Pf1N1B4_1864 0.70 +0.7 2,134,256 + Pf1N1B4_1864 0.78 -2.2 2,134,256 + Pf1N1B4_1864 0.78 -0.1 2,134,257 - Pf1N1B4_1864 0.78 -0.8 2,134,257 - Pf1N1B4_1864 0.78 -1.9 2,134,257 - Pf1N1B4_1864 0.78 +1.0 2,134,257 - Pf1N1B4_1864 0.78 +0.8 2,134,257 - Pf1N1B4_1864 0.78 +0.6 2,134,257 - Pf1N1B4_1864 0.78 -0.5 2,134,414 + +1.5 2,134,432 - +0.2 2,134,487 + -1.3 2,134,487 + -0.2 2,134,487 + +0.5 2,134,487 + +0.1 2,134,487 + -1.3 2,134,487 + +0.5 2,134,487 + +0.1 2,134,487 + +1.2 2,134,488 - +0.3 2,134,488 - +0.9 2,134,488 - +0.8 2,134,488 - +0.6 2,134,488 - -2.3 2,134,488 - -0.1 2,134,488 - +0.3 2,134,488 - -1.6 2,134,488 - +1.5 2,134,491 + +1.5 2,134,491 + -0.1 2,134,491 + +0.7 2,134,491 + -1.1 2,134,491 + -0.1 2,134,491 + -0.5 2,134,491 + -0.2 2,134,491 + +0.0 2,134,491 + -0.6 2,134,492 - -0.4 2,134,492 - +0.2 2,134,492 - -1.5 2,134,492 - +0.5 2,134,492 - -0.5 2,134,492 - +0.6 2,134,492 - +0.5 2,134,492 - +0.0 2,134,492 - -1.3 2,134,661 + Pf1N1B4_1865 0.26 -1.9 2,134,662 - Pf1N1B4_1865 0.26 -1.9 2,134,662 - Pf1N1B4_1865 0.26 -0.1 2,134,674 + Pf1N1B4_1865 0.28 -1.5 2,134,674 + Pf1N1B4_1865 0.28 -2.7 2,134,675 - Pf1N1B4_1865 0.28 -0.7 2,134,675 - Pf1N1B4_1865 0.28 -1.1 2,134,675 - +3.5 2,134,689 + Pf1N1B4_1865 0.30 +0.8 2,134,689 + Pf1N1B4_1865 0.30 -0.2 2,134,690 - Pf1N1B4_1865 0.30 -0.9 2,134,932 + Pf1N1B4_1865 0.66 -1.7 2,134,932 + Pf1N1B4_1865 0.66 -1.7 2,134,932 + Pf1N1B4_1865 0.66 -0.1 2,134,932 + Pf1N1B4_1865 0.66 -0.8 2,134,933 - Pf1N1B4_1865 0.66 +0.1 2,134,933 - Pf1N1B4_1865 0.66 -2.2 2,134,933 - Pf1N1B4_1865 0.66 -0.4 2,134,982 + Pf1N1B4_1865 0.73 -1.9 2,134,983 - Pf1N1B4_1865 0.74 +1.5 2,135,065 + Pf1N1B4_1865 0.86 -0.5 2,135,065 + Pf1N1B4_1865 0.86 -1.1 2,135,066 - Pf1N1B4_1865 0.86 -0.9 2,135,095 - -0.1 2,135,299 + Pf1N1B4_1866 0.14 -0.1 2,135,299 + Pf1N1B4_1866 0.14 -1.3 2,135,299 + Pf1N1B4_1866 0.14 -1.4 2,135,299 + Pf1N1B4_1866 0.14 -1.4 2,135,299 + Pf1N1B4_1866 0.14 +0.1 2,135,300 - Pf1N1B4_1866 0.14 +1.2 2,135,344 + Pf1N1B4_1866 0.18 -0.4 2,136,032 + Pf1N1B4_1866 0.87 -0.7 2,136,117 - -1.3
Or see this region's nucleotide sequence