Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1628 and Pf1N1B4_1629 are separated by 258 nucleotides Pf1N1B4_1629 and Pf1N1B4_1630 are separated by 77 nucleotides Pf1N1B4_1630 and Pf1N1B4_1631 overlap by 4 nucleotides
Pf1N1B4_1628 - MFS permease, at 1,871,107 to 1,872,771
_1628
Pf1N1B4_1629 - FIG00958480: hypothetical protein, at 1,873,030 to 1,873,434
_1629
Pf1N1B4_1630 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 1,873,512 to 1,875,746
_1630
Pf1N1B4_1631 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 1,875,743 to 1,876,810
_1631
Position (kb)
1873
1874
1875
1876 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1872.627 kb on - strand at 1872.765 kb on + strand at 1872.765 kb on + strand at 1872.765 kb on + strand at 1872.787 kb on + strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.790 kb on + strand at 1872.790 kb on + strand at 1872.790 kb on + strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.827 kb on - strand at 1872.852 kb on - strand at 1872.852 kb on - strand at 1872.947 kb on + strand at 1872.948 kb on - strand at 1872.961 kb on - strand at 1872.961 kb on - strand at 1872.978 kb on - strand at 1872.993 kb on + strand at 1873.067 kb on - strand at 1873.067 kb on - strand at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.465 kb on - strand at 1873.466 kb on + strand at 1873.466 kb on + strand at 1873.466 kb on + strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.543 kb on + strand at 1873.543 kb on + strand at 1873.544 kb on - strand at 1873.544 kb on - strand at 1873.544 kb on - strand at 1873.614 kb on + strand at 1873.614 kb on + strand at 1873.614 kb on + strand at 1873.614 kb on + strand at 1873.615 kb on - strand at 1873.615 kb on - strand at 1873.615 kb on - strand at 1873.615 kb on - strand at 1873.616 kb on + strand at 1873.617 kb on - strand at 1873.617 kb on - strand at 1874.062 kb on - strand, within Pf1N1B4_1630 at 1874.062 kb on - strand, within Pf1N1B4_1630 at 1874.245 kb on - strand, within Pf1N1B4_1630 at 1874.245 kb on - strand, within Pf1N1B4_1630 at 1874.710 kb on - strand, within Pf1N1B4_1630 at 1874.710 kb on - strand, within Pf1N1B4_1630 at 1875.010 kb on - strand, within Pf1N1B4_1630 at 1875.010 kb on - strand, within Pf1N1B4_1630 at 1875.036 kb on - strand, within Pf1N1B4_1630 at 1875.247 kb on + strand, within Pf1N1B4_1630 at 1875.248 kb on - strand, within Pf1N1B4_1630 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.894 kb on - strand, within Pf1N1B4_1631 at 1875.894 kb on - strand, within Pf1N1B4_1631 at 1875.894 kb on - strand, within Pf1N1B4_1631 at 1875.989 kb on + strand at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1876.255 kb on + strand, within Pf1N1B4_1631 at 1876.262 kb on + strand, within Pf1N1B4_1631 at 1876.262 kb on + strand, within Pf1N1B4_1631 at 1876.281 kb on - strand, within Pf1N1B4_1631 at 1876.281 kb on - strand, within Pf1N1B4_1631 at 1876.281 kb on - strand, within Pf1N1B4_1631 at 1876.642 kb on - strand, within Pf1N1B4_1631 at 1876.642 kb on - strand, within Pf1N1B4_1631 at 1876.712 kb on + strand at 1876.712 kb on + strand at 1876.712 kb on + strand at 1876.713 kb on - strand at 1876.713 kb on - strand at 1876.713 kb on - strand at 1876.713 kb on - strand at 1876.714 kb on + strand at 1876.715 kb on - strand at 1876.715 kb on - strand at 1876.715 kb on - strand at 1876.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 1,872,627 - +0.4 1,872,765 + -0.8 1,872,765 + +1.4 1,872,765 + +1.4 1,872,787 + +0.4 1,872,788 - -0.0 1,872,788 - -0.1 1,872,788 - -0.1 1,872,788 - -0.7 1,872,788 - +0.2 1,872,788 - +0.9 1,872,788 - -0.0 1,872,788 - -0.2 1,872,788 - -1.1 1,872,788 - -0.2 1,872,788 - -0.1 1,872,788 - -0.2 1,872,788 - -0.5 1,872,788 - -0.3 1,872,788 - -1.0 1,872,788 - -0.8 1,872,788 - -0.1 1,872,788 - -0.0 1,872,788 - +0.4 1,872,788 - +0.7 1,872,788 - +0.5 1,872,788 - -1.3 1,872,788 - -1.2 1,872,790 + -0.5 1,872,790 + +0.8 1,872,790 + -1.2 1,872,791 - -2.8 1,872,791 - +0.3 1,872,791 - +0.5 1,872,791 - -0.2 1,872,791 - -0.5 1,872,791 - +0.0 1,872,791 - -0.6 1,872,791 - -0.6 1,872,791 - +0.2 1,872,791 - -0.3 1,872,791 - +0.4 1,872,791 - -0.4 1,872,791 - +2.1 1,872,791 - -0.1 1,872,791 - -0.6 1,872,791 - -0.3 1,872,791 - -0.3 1,872,791 - +1.0 1,872,791 - +1.0 1,872,791 - +0.1 1,872,791 - -0.4 1,872,791 - -0.6 1,872,791 - +0.2 1,872,791 - -0.5 1,872,791 - -0.7 1,872,791 - +0.9 1,872,794 - +0.1 1,872,794 - -0.3 1,872,794 - -0.0 1,872,794 - +1.6 1,872,794 - -0.7 1,872,794 - -0.8 1,872,794 - +0.4 1,872,794 - -1.0 1,872,794 - -2.4 1,872,794 - -2.3 1,872,794 - +0.0 1,872,794 - +0.4 1,872,794 - -0.5 1,872,794 - -0.1 1,872,794 - -2.9 1,872,827 - +0.0 1,872,852 - +0.4 1,872,852 - -1.2 1,872,947 + -0.8 1,872,948 - +1.4 1,872,961 - -1.7 1,872,961 - -0.2 1,872,978 - +0.3 1,872,993 + +0.8 1,873,067 - +1.4 1,873,067 - +0.1 1,873,259 + Pf1N1B4_1629 0.57 +0.1 1,873,259 + Pf1N1B4_1629 0.57 -0.4 1,873,259 + Pf1N1B4_1629 0.57 +0.4 1,873,259 + Pf1N1B4_1629 0.57 -0.6 1,873,259 + Pf1N1B4_1629 0.57 +1.1 1,873,260 - Pf1N1B4_1629 0.57 -0.9 1,873,260 - Pf1N1B4_1629 0.57 -1.4 1,873,260 - Pf1N1B4_1629 0.57 +1.8 1,873,260 - Pf1N1B4_1629 0.57 -0.9 1,873,260 - Pf1N1B4_1629 0.57 -2.8 1,873,260 - Pf1N1B4_1629 0.57 +1.1 1,873,465 - -0.1 1,873,466 + -0.9 1,873,466 + -2.5 1,873,466 + -0.6 1,873,467 - +0.6 1,873,467 - -0.2 1,873,467 - -0.2 1,873,467 - +0.1 1,873,467 - -1.2 1,873,467 - -0.4 1,873,497 + +1.7 1,873,497 + +0.1 1,873,497 + -1.1 1,873,497 + -1.6 1,873,497 + +0.7 1,873,497 + -0.6 1,873,497 + -0.6 1,873,497 + -0.1 1,873,497 + -0.0 1,873,498 - -0.1 1,873,498 - +1.4 1,873,498 - -0.3 1,873,498 - -1.2 1,873,498 - -0.2 1,873,498 - +0.2 1,873,543 + -1.2 1,873,543 + +1.4 1,873,544 - +0.4 1,873,544 - -0.5 1,873,544 - -0.4 1,873,614 + -0.2 1,873,614 + -0.1 1,873,614 + +1.4 1,873,614 + +1.4 1,873,615 - -2.3 1,873,615 - -0.3 1,873,615 - -0.6 1,873,615 - +1.4 1,873,616 + -0.7 1,873,617 - -0.3 1,873,617 - +0.6 1,874,062 - Pf1N1B4_1630 0.25 -1.9 1,874,062 - Pf1N1B4_1630 0.25 -1.6 1,874,245 - Pf1N1B4_1630 0.33 +0.8 1,874,245 - Pf1N1B4_1630 0.33 -1.6 1,874,710 - Pf1N1B4_1630 0.54 -0.9 1,874,710 - Pf1N1B4_1630 0.54 +0.4 1,875,010 - Pf1N1B4_1630 0.67 -0.5 1,875,010 - Pf1N1B4_1630 0.67 -1.5 1,875,036 - Pf1N1B4_1630 0.68 -0.2 1,875,247 + Pf1N1B4_1630 0.78 -1.4 1,875,248 - Pf1N1B4_1630 0.78 -0.3 1,875,893 + Pf1N1B4_1631 0.14 +0.4 1,875,893 + Pf1N1B4_1631 0.14 -0.7 1,875,893 + Pf1N1B4_1631 0.14 +0.8 1,875,893 + Pf1N1B4_1631 0.14 +0.0 1,875,893 + Pf1N1B4_1631 0.14 -0.4 1,875,893 + Pf1N1B4_1631 0.14 +0.3 1,875,894 - Pf1N1B4_1631 0.14 +0.4 1,875,894 - Pf1N1B4_1631 0.14 -1.0 1,875,894 - Pf1N1B4_1631 0.14 -1.8 1,875,989 + +0.4 1,875,989 + Pf1N1B4_1631 0.23 +1.7 1,875,989 + Pf1N1B4_1631 0.23 -0.9 1,875,989 + Pf1N1B4_1631 0.23 -0.4 1,875,989 + Pf1N1B4_1631 0.23 +0.1 1,875,989 + Pf1N1B4_1631 0.23 -1.2 1,876,255 + Pf1N1B4_1631 0.48 -1.4 1,876,262 + Pf1N1B4_1631 0.49 +2.0 1,876,262 + Pf1N1B4_1631 0.49 -1.1 1,876,281 - Pf1N1B4_1631 0.50 +0.8 1,876,281 - Pf1N1B4_1631 0.50 +0.3 1,876,281 - Pf1N1B4_1631 0.50 -0.1 1,876,642 - Pf1N1B4_1631 0.84 +0.4 1,876,642 - Pf1N1B4_1631 0.84 -0.3 1,876,712 + -0.6 1,876,712 + -0.3 1,876,712 + -1.1 1,876,713 - -1.4 1,876,713 - -0.4 1,876,713 - +0.7 1,876,713 - +0.3 1,876,714 + -1.1 1,876,715 - -0.2 1,876,715 - +2.0 1,876,715 - -0.1 1,876,715 - +0.1
Or see this region's nucleotide sequence