Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1294 and Pf1N1B4_1295 are separated by 24 nucleotides Pf1N1B4_1295 and Pf1N1B4_1296 are separated by 257 nucleotides
Pf1N1B4_1294 - VgrG protein, at 1,475,067 to 1,476,122
_1294
Pf1N1B4_1295 - hypothetical protein, at 1,476,147 to 1,476,368
_1295
Pf1N1B4_1296 - Biosynthetic arginine decarboxylase (EC 4.1.1.19) (from data) , at 1,476,626 to 1,478,539
_1296
Position (kb)
1476
1477 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1475.601 kb on - strand, within Pf1N1B4_1294 at 1475.602 kb on + strand, within Pf1N1B4_1294 at 1475.603 kb on - strand, within Pf1N1B4_1294 at 1475.890 kb on + strand, within Pf1N1B4_1294 at 1476.030 kb on + strand at 1476.031 kb on - strand at 1476.031 kb on - strand at 1476.129 kb on + strand at 1476.130 kb on - strand at 1476.226 kb on + strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.351 kb on + strand at 1476.351 kb on + strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.449 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.469 kb on + strand at 1476.470 kb on - strand at 1476.470 kb on - strand at 1476.481 kb on + strand at 1476.481 kb on + strand at 1476.482 kb on - strand at 1476.583 kb on + strand at 1476.584 kb on - strand at 1476.584 kb on - strand at 1476.622 kb on + strand at 1476.622 kb on + strand at 1476.623 kb on - strand at 1476.623 kb on - strand at 1476.639 kb on + strand at 1476.639 kb on + strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.765 kb on + strand at 1476.813 kb on + strand at 1476.814 kb on - strand at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.927 kb on + strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.954 kb on - strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.245 kb on + strand, within Pf1N1B4_1296 at 1477.245 kb on + strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.296 kb on + strand, within Pf1N1B4_1296 at 1477.296 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.338 kb on + strand, within Pf1N1B4_1296
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 1,475,601 - Pf1N1B4_1294 0.51 -0.1 1,475,602 + Pf1N1B4_1294 0.51 -0.0 1,475,603 - Pf1N1B4_1294 0.51 +1.3 1,475,890 + Pf1N1B4_1294 0.78 +1.3 1,476,030 + +1.7 1,476,031 - -0.7 1,476,031 - -1.5 1,476,129 + -0.7 1,476,130 - -0.9 1,476,226 + Pf1N1B4_1295 0.36 -0.9 1,476,227 - Pf1N1B4_1295 0.36 -1.3 1,476,227 - Pf1N1B4_1295 0.36 -0.9 1,476,351 + -0.3 1,476,351 + -0.1 1,476,352 - +1.3 1,476,352 - -1.5 1,476,352 - -1.3 1,476,352 - +0.3 1,476,449 + -1.3 1,476,462 + +0.3 1,476,462 + +0.1 1,476,462 + -0.9 1,476,462 + -1.3 1,476,462 + +1.9 1,476,469 + -0.7 1,476,470 - -0.5 1,476,470 - -0.1 1,476,481 + +1.3 1,476,481 + +1.3 1,476,482 - -0.2 1,476,583 + -0.3 1,476,584 - -0.3 1,476,584 - -0.3 1,476,622 + +1.3 1,476,622 + -0.5 1,476,623 - +1.3 1,476,623 - -0.2 1,476,639 + -0.0 1,476,639 + -0.6 1,476,640 - -1.4 1,476,640 - -0.9 1,476,640 - -0.7 1,476,640 - +0.1 1,476,640 - -1.6 1,476,765 + -1.2 1,476,813 + -2.2 1,476,814 - -0.7 1,476,837 + Pf1N1B4_1296 0.11 +0.7 1,476,837 + Pf1N1B4_1296 0.11 +0.7 1,476,837 + Pf1N1B4_1296 0.11 -2.4 1,476,838 - Pf1N1B4_1296 0.11 -1.4 1,476,838 - Pf1N1B4_1296 0.11 -0.3 1,476,927 + Pf1N1B4_1296 0.16 -0.1 1,476,928 - Pf1N1B4_1296 0.16 -2.2 1,476,928 - Pf1N1B4_1296 0.16 +0.9 1,476,954 - Pf1N1B4_1296 0.17 -1.4 1,476,990 + Pf1N1B4_1296 0.19 -0.6 1,476,990 + Pf1N1B4_1296 0.19 -0.2 1,476,991 - Pf1N1B4_1296 0.19 +1.0 1,476,991 - Pf1N1B4_1296 0.19 -0.9 1,476,991 - Pf1N1B4_1296 0.19 -1.5 1,477,146 + Pf1N1B4_1296 0.27 +0.1 1,477,146 + Pf1N1B4_1296 0.27 +0.5 1,477,146 + Pf1N1B4_1296 0.27 -2.9 1,477,146 + Pf1N1B4_1296 0.27 -3.0 1,477,146 + Pf1N1B4_1296 0.27 -0.1 1,477,146 + Pf1N1B4_1296 0.27 +0.3 1,477,146 + Pf1N1B4_1296 0.27 +0.3 1,477,147 - Pf1N1B4_1296 0.27 -0.3 1,477,147 - Pf1N1B4_1296 0.27 -0.7 1,477,147 - Pf1N1B4_1296 0.27 -1.4 1,477,147 - Pf1N1B4_1296 0.27 -1.0 1,477,147 - Pf1N1B4_1296 0.27 +1.3 1,477,147 - Pf1N1B4_1296 0.27 +1.5 1,477,147 - Pf1N1B4_1296 0.27 -2.3 1,477,147 - Pf1N1B4_1296 0.27 -0.3 1,477,147 - Pf1N1B4_1296 0.27 +0.6 1,477,147 - Pf1N1B4_1296 0.27 -0.5 1,477,245 + Pf1N1B4_1296 0.32 -0.1 1,477,245 + Pf1N1B4_1296 0.32 -0.5 1,477,246 - Pf1N1B4_1296 0.32 -0.0 1,477,246 - Pf1N1B4_1296 0.32 -1.5 1,477,246 - Pf1N1B4_1296 0.32 +0.8 1,477,246 - Pf1N1B4_1296 0.32 +0.5 1,477,246 - Pf1N1B4_1296 0.32 +0.2 1,477,296 + Pf1N1B4_1296 0.35 -1.0 1,477,296 + Pf1N1B4_1296 0.35 -0.0 1,477,320 + Pf1N1B4_1296 0.36 +0.4 1,477,320 + -1.0 1,477,320 + Pf1N1B4_1296 0.36 +1.3 1,477,320 + Pf1N1B4_1296 0.36 -0.9 1,477,321 - Pf1N1B4_1296 0.36 -1.3 1,477,321 - Pf1N1B4_1296 0.36 +2.3 1,477,321 - Pf1N1B4_1296 0.36 -0.7 1,477,321 - Pf1N1B4_1296 0.36 +0.9 1,477,321 - Pf1N1B4_1296 0.36 -0.0 1,477,321 - -0.7 1,477,338 + Pf1N1B4_1296 0.37 +0.3 1,477,338 + Pf1N1B4_1296 0.37 -0.0 1,477,338 + Pf1N1B4_1296 0.37 -0.7
Or see this region's nucleotide sequence