Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1227 and Pf1N1B4_1228 are separated by 245 nucleotides Pf1N1B4_1228 and Pf1N1B4_1229 are separated by 10 nucleotides
Pf1N1B4_1227 - FIG00954870: hypothetical protein, at 1,393,478 to 1,393,822
_1227
Pf1N1B4_1228 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 1,394,068 to 1,394,955
_1228
Pf1N1B4_1229 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 1,394,966 to 1,396,483
_1229
Position (kb)
1394
1395 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1393.088 kb on + strand at 1393.144 kb on + strand at 1393.144 kb on + strand at 1393.181 kb on + strand at 1393.182 kb on - strand at 1393.188 kb on + strand at 1393.189 kb on - strand at 1393.214 kb on - strand at 1393.225 kb on + strand at 1393.227 kb on + strand at 1393.227 kb on + strand at 1393.227 kb on + strand at 1393.228 kb on - strand at 1393.228 kb on - strand at 1393.230 kb on + strand at 1393.253 kb on - strand at 1393.364 kb on + strand at 1393.364 kb on + strand at 1393.364 kb on + strand at 1393.387 kb on + strand at 1393.387 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.393 kb on + strand at 1393.394 kb on - strand at 1393.489 kb on + strand at 1393.489 kb on + strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.490 kb on - strand at 1393.500 kb on + strand at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.586 kb on + strand, within Pf1N1B4_1227 at 1393.587 kb on - strand, within Pf1N1B4_1227 at 1393.619 kb on - strand, within Pf1N1B4_1227 at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.646 kb on + strand at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.646 kb on + strand, within Pf1N1B4_1227 at 1393.647 kb on - strand at 1393.655 kb on + strand, within Pf1N1B4_1227 at 1393.656 kb on - strand, within Pf1N1B4_1227 at 1393.670 kb on + strand, within Pf1N1B4_1227 at 1393.692 kb on - strand, within Pf1N1B4_1227 at 1393.805 kb on + strand at 1393.806 kb on - strand at 1393.873 kb on + strand at 1393.873 kb on + strand at 1393.873 kb on + strand at 1393.874 kb on - strand at 1393.874 kb on - strand at 1393.958 kb on - strand at 1393.958 kb on - strand at 1393.978 kb on - strand at 1393.978 kb on - strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.145 kb on - strand at 1394.145 kb on - strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1395.051 kb on + strand at 1395.051 kb on + strand at 1395.193 kb on + strand, within Pf1N1B4_1229 at 1395.194 kb on - strand at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.610 kb on + strand, within Pf1N1B4_1229 at 1395.736 kb on + strand at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1395.760 kb on - strand, within Pf1N1B4_1229
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 1,393,088 + +0.0 1,393,144 + +0.7 1,393,144 + -0.3 1,393,181 + +2.0 1,393,182 - -0.8 1,393,188 + +0.6 1,393,189 - -1.8 1,393,214 - +1.7 1,393,225 + +1.8 1,393,227 + -0.0 1,393,227 + +2.2 1,393,227 + -0.8 1,393,228 - +0.2 1,393,228 - -0.3 1,393,230 + +1.0 1,393,253 - +0.2 1,393,364 + -0.9 1,393,364 + -0.8 1,393,364 + +0.4 1,393,387 + -0.2 1,393,387 + +1.2 1,393,393 + +1.0 1,393,393 + -0.5 1,393,393 + -0.2 1,393,393 + -0.5 1,393,393 + -1.6 1,393,393 + +0.2 1,393,393 + +0.4 1,393,393 + +1.0 1,393,394 - +0.8 1,393,489 + +1.8 1,393,489 + +0.4 1,393,490 - -1.3 1,393,490 - +0.7 1,393,490 - -0.2 1,393,490 - +0.5 1,393,500 + -0.6 1,393,586 + Pf1N1B4_1227 0.31 -1.9 1,393,586 + Pf1N1B4_1227 0.31 +0.8 1,393,586 + Pf1N1B4_1227 0.31 +1.2 1,393,587 - Pf1N1B4_1227 0.32 +1.2 1,393,619 - Pf1N1B4_1227 0.41 +0.8 1,393,646 + Pf1N1B4_1227 0.49 -1.1 1,393,646 + +1.2 1,393,646 + Pf1N1B4_1227 0.49 -0.8 1,393,646 + Pf1N1B4_1227 0.49 -0.1 1,393,647 - +0.7 1,393,655 + Pf1N1B4_1227 0.51 +0.4 1,393,656 - Pf1N1B4_1227 0.52 -0.1 1,393,670 + Pf1N1B4_1227 0.56 +0.2 1,393,692 - Pf1N1B4_1227 0.62 +1.7 1,393,805 + +2.2 1,393,806 - -0.4 1,393,873 + +1.2 1,393,873 + -0.3 1,393,873 + -0.2 1,393,874 - +0.1 1,393,874 - -0.3 1,393,958 - -0.3 1,393,958 - +1.7 1,393,978 - +1.2 1,393,978 - -0.3 1,394,144 + -0.3 1,394,144 + +0.7 1,394,144 + +0.2 1,394,144 + -1.8 1,394,145 - +2.2 1,394,145 - -0.8 1,394,303 + Pf1N1B4_1228 0.26 -3.5 1,394,303 + Pf1N1B4_1228 0.26 +0.2 1,394,303 + Pf1N1B4_1228 0.26 -0.2 1,394,303 + +0.7 1,394,303 + Pf1N1B4_1228 0.26 +0.7 1,394,304 - Pf1N1B4_1228 0.27 -1.1 1,394,304 - Pf1N1B4_1228 0.27 +0.0 1,394,304 - Pf1N1B4_1228 0.27 -1.0 1,394,304 - Pf1N1B4_1228 0.27 +0.2 1,394,304 - Pf1N1B4_1228 0.27 -2.2 1,394,304 - Pf1N1B4_1228 0.27 -1.9 1,394,304 - +0.7 1,394,304 - Pf1N1B4_1228 0.27 -0.3 1,394,304 - Pf1N1B4_1228 0.27 -1.2 1,395,051 + +0.1 1,395,051 + +0.2 1,395,193 + Pf1N1B4_1229 0.15 -0.4 1,395,194 - +1.2 1,395,327 + Pf1N1B4_1229 0.24 -1.5 1,395,327 + Pf1N1B4_1229 0.24 +0.2 1,395,327 + Pf1N1B4_1229 0.24 -1.3 1,395,327 + Pf1N1B4_1229 0.24 +0.9 1,395,327 + Pf1N1B4_1229 0.24 +0.7 1,395,327 + Pf1N1B4_1229 0.24 +0.2 1,395,327 + Pf1N1B4_1229 0.24 -1.8 1,395,328 - Pf1N1B4_1229 0.24 +2.1 1,395,328 - Pf1N1B4_1229 0.24 -1.9 1,395,610 + Pf1N1B4_1229 0.42 -0.6 1,395,736 + -0.3 1,395,760 - Pf1N1B4_1229 0.52 -0.9 1,395,760 - Pf1N1B4_1229 0.52 +0.2
Or see this region's nucleotide sequence