Experiment: Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1131 and Pf1N1B4_1132 are separated by 20 nucleotides Pf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotides Pf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotides
Pf1N1B4_1131 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,269,525 to 1,270,241
_1131
Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155
_1132
Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821
_1133
Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975
_1134
Position (kb)
1270
1271
1272 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1269.284 kb on - strand at 1269.284 kb on - strand at 1269.287 kb on + strand at 1269.287 kb on + strand at 1269.287 kb on + strand at 1269.288 kb on - strand at 1269.288 kb on - strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.297 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.301 kb on + strand at 1269.302 kb on - strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.313 kb on + strand at 1269.314 kb on - strand at 1269.314 kb on - strand at 1269.352 kb on + strand at 1269.352 kb on + strand at 1269.353 kb on - strand at 1269.399 kb on - strand at 1269.422 kb on - strand at 1269.537 kb on + strand at 1269.553 kb on + strand at 1269.553 kb on + strand at 1269.554 kb on - strand at 1269.645 kb on + strand, within Pf1N1B4_1131 at 1269.697 kb on + strand, within Pf1N1B4_1131 at 1269.697 kb on + strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.698 kb on - strand, within Pf1N1B4_1131 at 1269.806 kb on - strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.900 kb on + strand, within Pf1N1B4_1131 at 1269.901 kb on - strand, within Pf1N1B4_1131 at 1269.901 kb on - strand, within Pf1N1B4_1131 at 1269.977 kb on - strand, within Pf1N1B4_1131 at 1270.053 kb on - strand, within Pf1N1B4_1131 at 1270.239 kb on + strand at 1270.239 kb on + strand at 1270.239 kb on + strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.240 kb on - strand at 1270.278 kb on + strand at 1270.278 kb on + strand at 1270.278 kb on + strand at 1270.279 kb on - strand at 1270.279 kb on - strand at 1270.281 kb on + strand at 1270.281 kb on + strand at 1270.281 kb on + strand at 1270.282 kb on - strand at 1270.282 kb on - strand at 1270.282 kb on - strand at 1270.284 kb on + strand at 1270.285 kb on - strand at 1270.285 kb on - strand at 1270.285 kb on - strand at 1270.398 kb on - strand, within Pf1N1B4_1132 at 1270.415 kb on + strand, within Pf1N1B4_1132 at 1270.416 kb on - strand, within Pf1N1B4_1132 at 1270.523 kb on + strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.524 kb on - strand, within Pf1N1B4_1132 at 1270.634 kb on + strand, within Pf1N1B4_1132 at 1270.634 kb on + strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.635 kb on - strand, within Pf1N1B4_1132 at 1270.670 kb on + strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.998 kb on - strand, within Pf1N1B4_1132 at 1271.396 kb on + strand, within Pf1N1B4_1133 at 1271.397 kb on - strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1272.054 kb on + strand at 1272.054 kb on + strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.064 kb on + strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 200 mM remove 1,269,284 - -2.1 1,269,284 - +0.2 1,269,287 + -0.2 1,269,287 + -1.7 1,269,287 + -1.4 1,269,288 - +0.6 1,269,288 - -1.4 1,269,297 + +0.9 1,269,297 + +1.9 1,269,297 + +1.4 1,269,297 + -0.4 1,269,297 + +0.5 1,269,297 + +0.1 1,269,301 + +0.6 1,269,301 + -0.4 1,269,301 + +0.3 1,269,301 + +0.7 1,269,302 - -0.4 1,269,313 + -0.4 1,269,313 + -2.5 1,269,313 + +1.4 1,269,313 + +0.4 1,269,314 - -0.6 1,269,314 - +1.2 1,269,352 + +0.0 1,269,352 + +0.3 1,269,353 - +0.2 1,269,399 - -1.1 1,269,422 - -2.0 1,269,537 + -0.4 1,269,553 + +1.6 1,269,553 + -1.6 1,269,554 - -2.3 1,269,645 + Pf1N1B4_1131 0.17 +1.2 1,269,697 + Pf1N1B4_1131 0.24 -0.4 1,269,697 + Pf1N1B4_1131 0.24 -0.2 1,269,698 - Pf1N1B4_1131 0.24 +1.2 1,269,698 - Pf1N1B4_1131 0.24 -1.5 1,269,698 - Pf1N1B4_1131 0.24 -0.4 1,269,698 - Pf1N1B4_1131 0.24 -1.4 1,269,698 - Pf1N1B4_1131 0.24 +0.0 1,269,806 - Pf1N1B4_1131 0.39 +0.4 1,269,900 + Pf1N1B4_1131 0.52 -2.1 1,269,900 + Pf1N1B4_1131 0.52 -0.1 1,269,900 + Pf1N1B4_1131 0.52 +0.3 1,269,900 + Pf1N1B4_1131 0.52 -1.8 1,269,900 + Pf1N1B4_1131 0.52 -1.3 1,269,901 - Pf1N1B4_1131 0.52 -1.3 1,269,901 - Pf1N1B4_1131 0.52 +0.1 1,269,977 - Pf1N1B4_1131 0.63 -0.6 1,270,053 - Pf1N1B4_1131 0.74 -0.4 1,270,239 + -1.1 1,270,239 + +0.2 1,270,239 + -0.8 1,270,240 - -1.1 1,270,240 - +1.2 1,270,240 - -0.8 1,270,240 - +0.3 1,270,278 + -1.7 1,270,278 + -0.0 1,270,278 + -1.0 1,270,279 - -0.4 1,270,279 - +0.6 1,270,281 + -1.0 1,270,281 + -0.8 1,270,281 + +0.4 1,270,282 - +0.3 1,270,282 - +0.4 1,270,282 - -0.1 1,270,284 + -1.9 1,270,285 - +0.0 1,270,285 - -0.6 1,270,285 - -0.1 1,270,398 - Pf1N1B4_1132 0.15 -0.6 1,270,415 + Pf1N1B4_1132 0.17 -0.4 1,270,416 - Pf1N1B4_1132 0.17 +0.2 1,270,523 + Pf1N1B4_1132 0.29 +1.8 1,270,524 - Pf1N1B4_1132 0.29 -0.1 1,270,524 - Pf1N1B4_1132 0.29 +0.7 1,270,524 - Pf1N1B4_1132 0.29 -0.4 1,270,634 + Pf1N1B4_1132 0.42 -0.0 1,270,634 + Pf1N1B4_1132 0.42 +1.9 1,270,635 - Pf1N1B4_1132 0.42 -1.1 1,270,635 - Pf1N1B4_1132 0.42 -1.2 1,270,635 - Pf1N1B4_1132 0.42 +0.2 1,270,635 - Pf1N1B4_1132 0.42 -1.1 1,270,670 + Pf1N1B4_1132 0.46 +1.2 1,270,854 - Pf1N1B4_1132 0.66 -0.6 1,270,854 - Pf1N1B4_1132 0.66 +0.8 1,270,896 - +0.2 1,270,896 - Pf1N1B4_1132 0.71 -2.0 1,270,896 - Pf1N1B4_1132 0.71 -0.0 1,270,955 + Pf1N1B4_1132 0.78 +2.2 1,270,955 + Pf1N1B4_1132 0.78 -0.8 1,270,955 + Pf1N1B4_1132 0.78 +0.2 1,270,955 + Pf1N1B4_1132 0.78 -1.2 1,270,955 + -0.8 1,270,955 + Pf1N1B4_1132 0.78 +1.4 1,270,955 + Pf1N1B4_1132 0.78 -0.8 1,270,955 + Pf1N1B4_1132 0.78 +1.2 1,270,955 + Pf1N1B4_1132 0.78 +1.2 1,270,956 - Pf1N1B4_1132 0.78 -2.5 1,270,956 - Pf1N1B4_1132 0.78 -2.3 1,270,956 - Pf1N1B4_1132 0.78 +0.2 1,270,956 - Pf1N1B4_1132 0.78 -0.6 1,270,967 + Pf1N1B4_1132 0.79 -1.2 1,270,967 + Pf1N1B4_1132 0.79 -0.8 1,270,967 + Pf1N1B4_1132 0.79 -1.1 1,270,998 - Pf1N1B4_1132 0.82 +1.2 1,271,396 + Pf1N1B4_1133 0.35 -0.2 1,271,397 - Pf1N1B4_1133 0.35 -0.6 1,271,483 + Pf1N1B4_1133 0.48 -0.4 1,271,483 + -1.1 1,271,483 + Pf1N1B4_1133 0.48 -0.8 1,271,483 + Pf1N1B4_1133 0.48 +1.2 1,271,483 + Pf1N1B4_1133 0.48 -1.0 1,271,484 - Pf1N1B4_1133 0.48 -0.4 1,271,484 - Pf1N1B4_1133 0.48 -1.4 1,271,484 - Pf1N1B4_1133 0.48 +1.2 1,271,484 - Pf1N1B4_1133 0.48 -1.1 1,271,484 - Pf1N1B4_1133 0.48 -1.1 1,271,484 - Pf1N1B4_1133 0.48 -0.2 1,271,679 + Pf1N1B4_1133 0.78 -2.4 1,271,679 + Pf1N1B4_1133 0.78 -0.1 1,271,680 - Pf1N1B4_1133 0.78 +0.4 1,271,680 - Pf1N1B4_1133 0.78 -1.8 1,271,680 - Pf1N1B4_1133 0.78 -0.1 1,272,054 + -0.4 1,272,054 + Pf1N1B4_1134 0.20 -1.4 1,272,055 - Pf1N1B4_1134 0.20 +0.4 1,272,055 - Pf1N1B4_1134 0.20 -0.0 1,272,064 + Pf1N1B4_1134 0.21 -1.4 1,272,065 - Pf1N1B4_1134 0.21 -1.1 1,272,065 - Pf1N1B4_1134 0.21 -0.7
Or see this region's nucleotide sequence