Experiment: nitrate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1306 and SMa1308 overlap by 1 nucleotides SMa1308 and SMa1310 are separated by 4 nucleotides SMa1310 and SMa1311 overlap by 4 nucleotides
SMa1306: SMa1306 - VirB9 type IV secretion protein, at 713,420 to 714,274
SMa1306
SMa1308: SMa1308 - VirB8 type IV secretion protein, at 714,274 to 714,945
SMa1308
SMa1310: SMa1310 - VirB7-like protein, at 714,950 to 715,264
SMa1310
SMa1311: SMa1311 - VirB6 type IV secretion protein, at 715,261 to 716,202
SMa1311
Position (kb) 714
715
716 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 713.987 kb on - strand, within SMa1306 at 714.153 kb on + strand, within SMa1306 at 714.154 kb on - strand, within SMa1306 at 714.162 kb on + strand, within SMa1306 at 714.162 kb on + strand, within SMa1306 at 714.163 kb on - strand, within SMa1306 at 714.163 kb on - strand, within SMa1306 at 714.266 kb on - strand at 714.368 kb on + strand, within SMa1308 at 714.437 kb on + strand, within SMa1308 at 714.516 kb on + strand, within SMa1308 at 714.516 kb on + strand, within SMa1308 at 714.633 kb on - strand, within SMa1308 at 714.633 kb on - strand, within SMa1308 at 714.653 kb on + strand, within SMa1308 at 714.653 kb on + strand, within SMa1308 at 714.832 kb on + strand, within SMa1308 at 714.917 kb on + strand at 714.917 kb on + strand at 714.917 kb on + strand at 714.918 kb on - strand at 714.970 kb on - strand at 715.091 kb on - strand, within SMa1310 at 715.223 kb on - strand, within SMa1310 at 715.223 kb on - strand, within SMa1310 at 715.392 kb on - strand, within SMa1311 at 715.392 kb on - strand, within SMa1311 at 715.445 kb on + strand, within SMa1311 at 715.445 kb on + strand, within SMa1311 at 715.446 kb on - strand, within SMa1311 at 715.446 kb on - strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.454 kb on + strand, within SMa1311 at 715.455 kb on - strand, within SMa1311 at 715.455 kb on - strand, within SMa1311 at 715.455 kb on - strand, within SMa1311 at 715.509 kb on + strand, within SMa1311 at 715.509 kb on + strand, within SMa1311 at 715.509 kb on + strand, within SMa1311 at 715.510 kb on - strand, within SMa1311 at 715.510 kb on - strand, within SMa1311 at 715.556 kb on - strand, within SMa1311 at 715.722 kb on + strand, within SMa1311 at 715.722 kb on + strand, within SMa1311 at 715.725 kb on - strand, within SMa1311 at 715.943 kb on + strand, within SMa1311 at 715.943 kb on + strand, within SMa1311 at 715.943 kb on + strand, within SMa1311 at 715.944 kb on - strand, within SMa1311 at 715.944 kb on - strand, within SMa1311 at 716.061 kb on - strand, within SMa1311 at 716.061 kb on - strand, within SMa1311 at 716.072 kb on + strand, within SMa1311 at 716.192 kb on + strand at 716.192 kb on + strand at 716.192 kb on + strand at 716.192 kb on + strand at 716.193 kb on - strand at 716.193 kb on - strand at 716.193 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 40 mM remove 713,987 - SMa1306 0.66 -0.3 714,153 + SMa1306 0.86 +0.5 714,154 - SMa1306 0.86 +0.2 714,162 + SMa1306 0.87 -1.5 714,162 + SMa1306 0.87 +1.7 714,163 - SMa1306 0.87 +1.2 714,163 - SMa1306 0.87 -0.6 714,266 - -0.3 714,368 + SMa1308 0.14 -0.4 714,437 + SMa1308 0.24 +0.1 714,516 + SMa1308 0.36 -2.0 714,516 + SMa1308 0.36 -0.1 714,633 - SMa1308 0.53 -0.0 714,633 - SMa1308 0.53 +0.7 714,653 + SMa1308 0.56 +0.7 714,653 + SMa1308 0.56 +0.5 714,832 + SMa1308 0.83 +1.0 714,917 + -0.1 714,917 + -1.6 714,917 + -0.1 714,918 - +2.1 714,970 - +1.4 715,091 - SMa1310 0.45 -1.8 715,223 - SMa1310 0.87 -0.7 715,223 - SMa1310 0.87 -1.2 715,392 - SMa1311 0.14 +0.8 715,392 - SMa1311 0.14 +1.0 715,445 + SMa1311 0.20 -0.4 715,445 + SMa1311 0.20 +0.5 715,446 - SMa1311 0.20 +0.7 715,446 - SMa1311 0.20 -0.8 715,454 + SMa1311 0.20 +0.2 715,454 + SMa1311 0.20 -0.2 715,454 + SMa1311 0.20 -0.0 715,454 + SMa1311 0.20 +0.7 715,454 + SMa1311 0.20 -0.8 715,455 - SMa1311 0.21 -0.8 715,455 - SMa1311 0.21 +0.8 715,455 - SMa1311 0.21 -2.9 715,509 + SMa1311 0.26 +0.7 715,509 + SMa1311 0.26 -0.1 715,509 + SMa1311 0.26 -0.3 715,510 - SMa1311 0.26 -0.2 715,510 - SMa1311 0.26 +0.1 715,556 - SMa1311 0.31 -0.8 715,722 + SMa1311 0.49 -0.0 715,722 + SMa1311 0.49 +0.0 715,725 - SMa1311 0.49 +0.2 715,943 + SMa1311 0.72 +1.4 715,943 + SMa1311 0.72 +0.6 715,943 + SMa1311 0.72 -0.8 715,944 - SMa1311 0.73 +0.2 715,944 - SMa1311 0.73 -0.8 716,061 - SMa1311 0.85 -0.5 716,061 - SMa1311 0.85 +0.1 716,072 + SMa1311 0.86 +1.2 716,192 + +0.1 716,192 + +0.4 716,192 + -3.0 716,192 + -1.0 716,193 - +0.2 716,193 - +0.7 716,193 - -1.2
Or see this region's nucleotide sequence