Experiment: nitrate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0499 and SMa0501 are separated by 42 nucleotides SMa0501 and SMa0503 are separated by 1 nucleotides SMa0503 and SMa0506 are separated by 57 nucleotides SMa0506 and SMa0508 are separated by 49 nucleotides
SMa0499: SMa0499 - hypothetical protein, at 272,059 to 272,691
SMa0499
SMa0501: SMa0501 - ABC transporter permease, at 272,734 to 273,420
SMa0501
SMa0503: SMa0503 - ABC transporter permease, at 273,422 to 274,078
SMa0503
SMa0506: SMa0506 - ABC transporter periplasmic substrate-binding protein, at 274,136 to 274,933
SMa0506
SMa0508: SMa0508 - ABC transporter ATP-binding protein, at 274,983 to 275,714
SMa0508
Position (kb) 273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 272.515 kb on - strand, within SMa0499 at 272.569 kb on + strand, within SMa0499 at 272.569 kb on + strand, within SMa0499 at 272.569 kb on + strand, within SMa0499 at 272.569 kb on + strand, within SMa0499 at 272.569 kb on + strand, within SMa0499 at 272.570 kb on - strand, within SMa0499 at 272.570 kb on - strand, within SMa0499 at 272.570 kb on - strand, within SMa0499 at 272.570 kb on - strand, within SMa0499 at 272.570 kb on - strand, within SMa0499 at 272.570 kb on - strand, within SMa0499 at 272.689 kb on + strand at 272.690 kb on - strand at 272.740 kb on + strand at 272.740 kb on + strand at 272.740 kb on + strand at 272.740 kb on + strand at 272.741 kb on - strand at 272.742 kb on + strand at 272.742 kb on + strand at 272.742 kb on + strand at 272.742 kb on + strand at 272.743 kb on - strand at 272.743 kb on - strand at 272.743 kb on - strand at 272.743 kb on - strand at 272.743 kb on - strand at 272.743 kb on - strand at 272.770 kb on + strand at 272.771 kb on - strand at 272.912 kb on - strand, within SMa0501 at 273.120 kb on + strand at 273.120 kb on + strand, within SMa0501 at 273.120 kb on + strand, within SMa0501 at 273.142 kb on + strand, within SMa0501 at 273.143 kb on - strand, within SMa0501 at 273.325 kb on + strand, within SMa0501 at 273.325 kb on + strand, within SMa0501 at 273.325 kb on + strand, within SMa0501 at 273.326 kb on - strand, within SMa0501 at 273.326 kb on - strand, within SMa0501 at 273.462 kb on - strand at 273.462 kb on - strand at 273.462 kb on - strand at 273.584 kb on + strand, within SMa0503 at 273.584 kb on + strand, within SMa0503 at 273.584 kb on + strand, within SMa0503 at 273.585 kb on - strand, within SMa0503 at 273.585 kb on - strand, within SMa0503 at 273.585 kb on - strand, within SMa0503 at 273.635 kb on + strand, within SMa0503 at 273.635 kb on + strand, within SMa0503 at 273.638 kb on + strand, within SMa0503 at 273.639 kb on - strand, within SMa0503 at 273.800 kb on + strand, within SMa0503 at 273.905 kb on - strand, within SMa0503 at 273.998 kb on + strand, within SMa0503 at 273.998 kb on + strand, within SMa0503 at 274.234 kb on + strand, within SMa0506 at 274.454 kb on + strand, within SMa0506 at 274.493 kb on + strand, within SMa0506 at 274.494 kb on - strand, within SMa0506 at 274.716 kb on - strand, within SMa0506 at 274.716 kb on - strand, within SMa0506 at 274.716 kb on - strand, within SMa0506 at 274.776 kb on - strand, within SMa0506 at 274.789 kb on - strand, within SMa0506 at 274.790 kb on + strand, within SMa0506 at 274.790 kb on + strand, within SMa0506 at 275.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 40 mM remove 272,515 - SMa0499 0.72 +1.3 272,569 + SMa0499 0.81 -0.7 272,569 + SMa0499 0.81 -0.3 272,569 + SMa0499 0.81 -0.5 272,569 + SMa0499 0.81 -0.7 272,569 + SMa0499 0.81 -1.1 272,570 - SMa0499 0.81 -2.9 272,570 - SMa0499 0.81 -0.2 272,570 - SMa0499 0.81 -0.7 272,570 - SMa0499 0.81 +0.0 272,570 - SMa0499 0.81 +0.1 272,570 - SMa0499 0.81 +0.0 272,689 + -0.6 272,690 - +0.9 272,740 + +0.3 272,740 + +0.9 272,740 + +0.9 272,740 + -1.0 272,741 - -0.7 272,742 + -0.7 272,742 + +1.3 272,742 + -1.3 272,742 + -1.0 272,743 - -2.8 272,743 - +0.5 272,743 - +0.8 272,743 - +0.0 272,743 - -1.7 272,743 - +1.3 272,770 + +0.7 272,771 - -2.9 272,912 - SMa0501 0.26 +0.5 273,120 + +1.3 273,120 + SMa0501 0.56 -1.0 273,120 + SMa0501 0.56 -0.2 273,142 + SMa0501 0.59 +0.1 273,143 - SMa0501 0.60 +0.2 273,325 + SMa0501 0.86 -0.6 273,325 + SMa0501 0.86 +0.5 273,325 + SMa0501 0.86 +0.1 273,326 - SMa0501 0.86 -0.7 273,326 - SMa0501 0.86 -3.3 273,462 - -2.5 273,462 - +1.3 273,462 - -0.3 273,584 + SMa0503 0.25 -0.7 273,584 + SMa0503 0.25 +2.3 273,584 + SMa0503 0.25 -0.0 273,585 - SMa0503 0.25 +0.3 273,585 - SMa0503 0.25 -0.0 273,585 - SMa0503 0.25 -2.6 273,635 + SMa0503 0.32 -2.3 273,635 + SMa0503 0.32 -3.4 273,638 + SMa0503 0.33 -1.2 273,639 - SMa0503 0.33 +0.6 273,800 + SMa0503 0.58 -1.4 273,905 - SMa0503 0.74 -1.5 273,998 + SMa0503 0.88 -1.5 273,998 + SMa0503 0.88 -3.0 274,234 + SMa0506 0.12 -3.3 274,454 + SMa0506 0.40 -2.2 274,493 + SMa0506 0.45 -0.0 274,494 - SMa0506 0.45 +0.5 274,716 - SMa0506 0.73 -0.9 274,716 - SMa0506 0.73 +1.1 274,716 - SMa0506 0.73 +0.2 274,776 - SMa0506 0.80 +0.6 274,789 - SMa0506 0.82 +0.0 274,790 + SMa0506 0.82 -1.4 274,790 + SMa0506 0.82 -0.7 275,047 + +0.6
Or see this region's nucleotide sequence