Experiment: nitrate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20247 and SM_b20248 are separated by 29 nucleotides SM_b20248 and SM_b20249 are separated by 151 nucleotides
SM_b20247: SM_b20247 - oxidoreductase, at 252,295 to 253,308
_b20247
SM_b20248: SM_b20248 - hypothetical protein, at 253,338 to 255,086
_b20248
SM_b20249: SM_b20249 - myo-inositol-1-phosphate synthase, at 255,238 to 256,341
_b20249
Position (kb) 253
254
255
256 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 252.697 kb on + strand, within SM_b20247 at 252.698 kb on - strand, within SM_b20247 at 252.787 kb on + strand, within SM_b20247 at 252.879 kb on - strand, within SM_b20247 at 252.879 kb on - strand, within SM_b20247 at 252.879 kb on - strand, within SM_b20247 at 252.973 kb on + strand, within SM_b20247 at 253.075 kb on + strand, within SM_b20247 at 253.075 kb on + strand, within SM_b20247 at 253.076 kb on - strand, within SM_b20247 at 253.101 kb on + strand, within SM_b20247 at 253.121 kb on - strand, within SM_b20247 at 253.297 kb on + strand at 253.307 kb on - strand at 253.340 kb on - strand at 253.340 kb on - strand at 253.375 kb on + strand at 253.465 kb on + strand at 253.891 kb on + strand, within SM_b20248 at 254.179 kb on - strand, within SM_b20248 at 254.687 kb on - strand, within SM_b20248 at 254.707 kb on + strand, within SM_b20248 at 254.708 kb on - strand, within SM_b20248 at 254.708 kb on - strand, within SM_b20248 at 254.708 kb on - strand, within SM_b20248 at 255.040 kb on - strand at 255.079 kb on - strand at 255.079 kb on - strand at 255.079 kb on - strand at 255.211 kb on + strand at 255.211 kb on + strand at 255.211 kb on + strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.216 kb on - strand at 255.316 kb on + strand at 255.317 kb on - strand at 255.317 kb on - strand at 255.457 kb on + strand, within SM_b20249 at 255.458 kb on - strand, within SM_b20249 at 255.640 kb on + strand, within SM_b20249 at 255.641 kb on - strand, within SM_b20249 at 255.676 kb on + strand, within SM_b20249 at 255.801 kb on - strand, within SM_b20249 at 255.872 kb on + strand, within SM_b20249 at 255.873 kb on - strand, within SM_b20249 at 255.873 kb on - strand, within SM_b20249 at 255.884 kb on - strand, within SM_b20249 at 255.906 kb on + strand, within SM_b20249 at 256.067 kb on - strand, within SM_b20249 at 256.067 kb on - strand, within SM_b20249 at 256.068 kb on + strand, within SM_b20249
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 40 mM remove 252,697 + SM_b20247 0.40 +0.1 252,698 - SM_b20247 0.40 +0.2 252,787 + SM_b20247 0.49 -0.2 252,879 - SM_b20247 0.58 -0.2 252,879 - SM_b20247 0.58 +0.2 252,879 - SM_b20247 0.58 +0.6 252,973 + SM_b20247 0.67 -0.1 253,075 + SM_b20247 0.77 +1.5 253,075 + SM_b20247 0.77 +0.8 253,076 - SM_b20247 0.77 -0.5 253,101 + SM_b20247 0.79 -0.2 253,121 - SM_b20247 0.81 +0.1 253,297 + -0.3 253,307 - +0.2 253,340 - +3.5 253,340 - -0.6 253,375 + -0.8 253,465 + -2.7 253,891 + SM_b20248 0.32 +0.5 254,179 - SM_b20248 0.48 +1.2 254,687 - SM_b20248 0.77 -0.7 254,707 + SM_b20248 0.78 +0.2 254,708 - SM_b20248 0.78 +1.3 254,708 - SM_b20248 0.78 -0.6 254,708 - SM_b20248 0.78 -4.2 255,040 - +1.1 255,079 - +0.3 255,079 - +0.1 255,079 - -1.3 255,211 + -0.7 255,211 + +0.8 255,211 + +0.7 255,212 - +0.7 255,212 - -0.0 255,212 - -1.7 255,212 - -2.0 255,212 - -0.5 255,212 - -1.5 255,216 - +2.0 255,316 + +0.6 255,317 - +0.4 255,317 - -2.8 255,457 + SM_b20249 0.20 -1.5 255,458 - SM_b20249 0.20 -3.8 255,640 + SM_b20249 0.36 +1.5 255,641 - SM_b20249 0.37 +0.1 255,676 + SM_b20249 0.40 +0.4 255,801 - SM_b20249 0.51 +0.1 255,872 + SM_b20249 0.57 -3.3 255,873 - SM_b20249 0.58 -0.7 255,873 - SM_b20249 0.58 -0.1 255,884 - SM_b20249 0.59 -1.0 255,906 + SM_b20249 0.61 -1.5 256,067 - SM_b20249 0.75 +1.8 256,067 - SM_b20249 0.75 -1.1 256,068 + SM_b20249 0.75 -0.0
Or see this region's nucleotide sequence