Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS11400 and ABZR86_RS11405 are separated by 38 nucleotides ABZR86_RS11405 and ABZR86_RS11410 overlap by 4 nucleotides ABZR86_RS11410 and ABZR86_RS11415 are separated by 18 nucleotides
ABZR86_RS11400: ABZR86_RS11400 - virB8 family protein, at 2,545,485 to 2,546,462
_RS11400
ABZR86_RS11405: ABZR86_RS11405 - TrbG/VirB9 family P-type conjugative transfer protein, at 2,546,501 to 2,547,229
_RS11405
ABZR86_RS11410: ABZR86_RS11410 - TrbI/VirB10 family protein, at 2,547,226 to 2,548,470
_RS11410
ABZR86_RS11415: ABZR86_RS11415 - P-type DNA transfer ATPase VirB11, at 2,548,489 to 2,549,523
_RS11415
Position (kb)
2547
2548
2549 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2546.433 kb on - strand at 2546.479 kb on - strand at 2546.552 kb on + strand at 2546.553 kb on - strand at 2546.553 kb on - strand at 2546.576 kb on + strand, within ABZR86_RS11405 at 2546.577 kb on - strand, within ABZR86_RS11405 at 2546.577 kb on - strand, within ABZR86_RS11405 at 2546.645 kb on + strand, within ABZR86_RS11405 at 2546.699 kb on + strand, within ABZR86_RS11405 at 2546.700 kb on - strand, within ABZR86_RS11405 at 2546.762 kb on + strand, within ABZR86_RS11405 at 2546.762 kb on + strand, within ABZR86_RS11405 at 2546.762 kb on + strand, within ABZR86_RS11405 at 2546.763 kb on - strand, within ABZR86_RS11405 at 2546.763 kb on - strand, within ABZR86_RS11405 at 2546.763 kb on - strand, within ABZR86_RS11405 at 2546.763 kb on - strand, within ABZR86_RS11405 at 2546.834 kb on + strand, within ABZR86_RS11405 at 2546.853 kb on - strand, within ABZR86_RS11405 at 2546.940 kb on - strand, within ABZR86_RS11405 at 2546.957 kb on + strand, within ABZR86_RS11405 at 2547.009 kb on - strand, within ABZR86_RS11405 at 2547.029 kb on + strand, within ABZR86_RS11405 at 2547.029 kb on + strand, within ABZR86_RS11405 at 2547.030 kb on - strand, within ABZR86_RS11405 at 2547.030 kb on - strand, within ABZR86_RS11405 at 2547.083 kb on + strand, within ABZR86_RS11405 at 2547.164 kb on + strand at 2547.164 kb on + strand at 2547.165 kb on - strand at 2547.201 kb on + strand at 2547.201 kb on + strand at 2547.202 kb on - strand at 2547.244 kb on + strand at 2547.245 kb on - strand at 2547.245 kb on - strand at 2547.317 kb on - strand at 2547.328 kb on + strand at 2547.328 kb on + strand at 2547.328 kb on + strand at 2547.328 kb on + strand at 2547.329 kb on - strand at 2547.329 kb on - strand at 2547.761 kb on - strand, within ABZR86_RS11410 at 2547.913 kb on + strand, within ABZR86_RS11410 at 2547.913 kb on + strand, within ABZR86_RS11410 at 2547.914 kb on - strand, within ABZR86_RS11410 at 2547.992 kb on - strand, within ABZR86_RS11410 at 2548.407 kb on + strand at 2548.423 kb on + strand at 2548.423 kb on + strand at 2548.423 kb on + strand at 2548.541 kb on - strand at 2548.597 kb on - strand, within ABZR86_RS11415 at 2548.597 kb on - strand, within ABZR86_RS11415 at 2548.597 kb on - strand, within ABZR86_RS11415 at 2548.619 kb on - strand, within ABZR86_RS11415 at 2548.619 kb on - strand, within ABZR86_RS11415 at 2548.652 kb on - strand, within ABZR86_RS11415 at 2548.870 kb on + strand, within ABZR86_RS11415 at 2548.871 kb on - strand, within ABZR86_RS11415 at 2548.953 kb on + strand, within ABZR86_RS11415 at 2548.996 kb on + strand, within ABZR86_RS11415 at 2548.996 kb on + strand, within ABZR86_RS11415 at 2548.997 kb on - strand, within ABZR86_RS11415 at 2548.997 kb on - strand, within ABZR86_RS11415 at 2548.997 kb on - strand, within ABZR86_RS11415 at 2549.152 kb on + strand, within ABZR86_RS11415
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 2,546,433 - +0.4 2,546,479 - -0.4 2,546,552 + +0.5 2,546,553 - +0.3 2,546,553 - -0.4 2,546,576 + ABZR86_RS11405 0.10 -0.6 2,546,577 - ABZR86_RS11405 0.10 -0.0 2,546,577 - ABZR86_RS11405 0.10 -0.3 2,546,645 + ABZR86_RS11405 0.20 -0.4 2,546,699 + ABZR86_RS11405 0.27 -1.7 2,546,700 - ABZR86_RS11405 0.27 -0.5 2,546,762 + ABZR86_RS11405 0.36 -1.3 2,546,762 + ABZR86_RS11405 0.36 +0.2 2,546,762 + ABZR86_RS11405 0.36 -1.5 2,546,763 - ABZR86_RS11405 0.36 +0.3 2,546,763 - ABZR86_RS11405 0.36 +0.9 2,546,763 - ABZR86_RS11405 0.36 +0.8 2,546,763 - ABZR86_RS11405 0.36 -0.4 2,546,834 + ABZR86_RS11405 0.46 +0.2 2,546,853 - ABZR86_RS11405 0.48 -0.1 2,546,940 - ABZR86_RS11405 0.60 +0.5 2,546,957 + ABZR86_RS11405 0.63 -0.2 2,547,009 - ABZR86_RS11405 0.70 +0.5 2,547,029 + ABZR86_RS11405 0.72 -1.7 2,547,029 + ABZR86_RS11405 0.72 -1.0 2,547,030 - ABZR86_RS11405 0.73 -0.5 2,547,030 - ABZR86_RS11405 0.73 +0.5 2,547,083 + ABZR86_RS11405 0.80 -0.1 2,547,164 + -1.1 2,547,164 + -0.4 2,547,165 - +0.1 2,547,201 + -0.6 2,547,201 + -0.6 2,547,202 - +0.4 2,547,244 + +0.1 2,547,245 - +0.4 2,547,245 - +0.1 2,547,317 - -0.0 2,547,328 + +0.2 2,547,328 + -0.2 2,547,328 + +0.1 2,547,328 + -0.0 2,547,329 - -0.4 2,547,329 - -0.1 2,547,761 - ABZR86_RS11410 0.43 -0.2 2,547,913 + ABZR86_RS11410 0.55 -0.6 2,547,913 + ABZR86_RS11410 0.55 +0.6 2,547,914 - ABZR86_RS11410 0.55 -0.1 2,547,992 - ABZR86_RS11410 0.62 -0.3 2,548,407 + -0.2 2,548,423 + -0.9 2,548,423 + -0.5 2,548,423 + -0.0 2,548,541 - +0.9 2,548,597 - ABZR86_RS11415 0.10 -0.7 2,548,597 - ABZR86_RS11415 0.10 -0.4 2,548,597 - ABZR86_RS11415 0.10 -0.4 2,548,619 - ABZR86_RS11415 0.13 +0.2 2,548,619 - ABZR86_RS11415 0.13 +0.0 2,548,652 - ABZR86_RS11415 0.16 +0.2 2,548,870 + ABZR86_RS11415 0.37 +0.3 2,548,871 - ABZR86_RS11415 0.37 +0.4 2,548,953 + ABZR86_RS11415 0.45 -2.9 2,548,996 + ABZR86_RS11415 0.49 -0.8 2,548,996 + ABZR86_RS11415 0.49 +0.7 2,548,997 - ABZR86_RS11415 0.49 +0.0 2,548,997 - ABZR86_RS11415 0.49 -0.3 2,548,997 - ABZR86_RS11415 0.49 -1.3 2,549,152 + ABZR86_RS11415 0.64 +0.8
Or see this region's nucleotide sequence