Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_654 and Pf1N1B4_655 overlap by 4 nucleotides Pf1N1B4_655 and Pf1N1B4_656 are separated by 164 nucleotides Pf1N1B4_656 and Pf1N1B4_657 are separated by 133 nucleotides
Pf1N1B4_654 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 748,397 to 749,341
_654
Pf1N1B4_655 - Glutathione S-transferase (EC 2.5.1.18), at 749,338 to 749,970
_655
Pf1N1B4_656 - Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1), at 750,135 to 751,352
_656
Pf1N1B4_657 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 751,486 to 754,221
_657
Position (kb) 750
751
752 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 749.324 kb on + strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.347 kb on + strand at 749.347 kb on + strand at 749.348 kb on - strand at 749.348 kb on - strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.397 kb on - strand at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.437 kb on + strand, within Pf1N1B4_655 at 749.437 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.459 kb on - strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.750 kb on + strand, within Pf1N1B4_655 at 749.751 kb on - strand, within Pf1N1B4_655 at 749.882 kb on + strand, within Pf1N1B4_655 at 749.989 kb on - strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.145 kb on + strand at 750.166 kb on + strand at 750.166 kb on + strand at 750.167 kb on - strand at 750.167 kb on - strand at 750.167 kb on - strand at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.775 kb on + strand, within Pf1N1B4_656 at 750.776 kb on - strand, within Pf1N1B4_656 at 750.842 kb on + strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 750.843 kb on - strand, within Pf1N1B4_656 at 751.016 kb on - strand at 751.138 kb on + strand, within Pf1N1B4_656 at 751.139 kb on - strand, within Pf1N1B4_656 at 751.143 kb on + strand, within Pf1N1B4_656 at 751.365 kb on + strand at 751.366 kb on - strand at 751.372 kb on + strand at 751.372 kb on + strand at 751.372 kb on + strand at 751.372 kb on + strand at 751.373 kb on - strand at 751.373 kb on - strand at 751.373 kb on - strand at 751.401 kb on + strand at 751.401 kb on + strand at 751.401 kb on + strand at 751.401 kb on + strand at 751.402 kb on - strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.419 kb on + strand at 751.444 kb on + strand at 751.445 kb on - strand at 751.445 kb on - strand at 751.445 kb on - strand at 751.474 kb on + strand at 751.475 kb on - strand at 751.475 kb on - strand at 751.475 kb on - strand at 751.487 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 749,324 + +0.2 749,325 - -0.0 749,325 - -0.5 749,325 - -1.0 749,325 - -0.1 749,325 - +0.5 749,325 - +0.1 749,347 + +1.0 749,347 + +0.3 749,348 - +1.6 749,348 - -0.1 749,396 + -0.6 749,396 + -0.8 749,396 + +0.7 749,396 + +0.2 749,396 + -0.6 749,396 + +1.3 749,397 - -1.0 749,411 + Pf1N1B4_655 0.12 +0.6 749,411 + Pf1N1B4_655 0.12 -0.9 749,411 + Pf1N1B4_655 0.12 -0.6 749,411 + Pf1N1B4_655 0.12 -0.5 749,412 - Pf1N1B4_655 0.12 -0.0 749,412 - Pf1N1B4_655 0.12 +0.7 749,412 - Pf1N1B4_655 0.12 -0.0 749,412 - Pf1N1B4_655 0.12 -0.9 749,412 - Pf1N1B4_655 0.12 -0.3 749,437 + Pf1N1B4_655 0.16 +1.0 749,437 + Pf1N1B4_655 0.16 -0.8 749,444 + Pf1N1B4_655 0.17 -0.0 749,444 + Pf1N1B4_655 0.17 +0.2 749,444 + Pf1N1B4_655 0.17 +1.0 749,444 + Pf1N1B4_655 0.17 -0.2 749,445 - Pf1N1B4_655 0.17 +0.6 749,445 - Pf1N1B4_655 0.17 -0.6 749,445 - Pf1N1B4_655 0.17 -0.0 749,445 - Pf1N1B4_655 0.17 -1.8 749,445 - Pf1N1B4_655 0.17 +1.5 749,459 - Pf1N1B4_655 0.19 +0.7 749,634 + Pf1N1B4_655 0.47 -0.1 749,634 + Pf1N1B4_655 0.47 +1.2 749,634 + Pf1N1B4_655 0.47 -0.3 749,635 - Pf1N1B4_655 0.47 -0.3 749,635 - Pf1N1B4_655 0.47 +0.7 749,635 - Pf1N1B4_655 0.47 +0.4 749,635 - Pf1N1B4_655 0.47 +0.5 749,635 - Pf1N1B4_655 0.47 -0.3 749,750 + Pf1N1B4_655 0.65 +0.9 749,751 - Pf1N1B4_655 0.65 +1.0 749,882 + Pf1N1B4_655 0.86 -1.2 749,989 - +0.8 750,136 + +0.6 750,136 + +0.7 750,136 + +1.7 750,136 + -0.3 750,136 + -0.0 750,137 - -0.0 750,137 - +1.7 750,137 - +0.7 750,137 - -0.3 750,137 - +0.7 750,140 + -2.3 750,140 + -0.3 750,140 + -0.6 750,140 + +1.3 750,140 + +0.3 750,140 + -0.7 750,140 + -1.0 750,140 + -0.3 750,140 + +0.1 750,140 + -1.4 750,140 + -0.8 750,140 + -0.0 750,140 + -0.0 750,140 + -1.0 750,140 + -0.7 750,141 - -1.1 750,141 - -0.6 750,141 - -0.6 750,141 - -0.2 750,141 - -2.6 750,141 - -1.8 750,141 - +0.4 750,141 - -0.1 750,141 - -0.0 750,141 - -0.4 750,141 - -1.3 750,141 - -0.9 750,141 - +0.8 750,141 - +0.2 750,141 - -1.0 750,145 + -1.2 750,166 + -1.5 750,166 + -2.0 750,167 - -1.0 750,167 - -0.6 750,167 - -1.1 750,280 + Pf1N1B4_656 0.12 -0.6 750,280 + Pf1N1B4_656 0.12 -0.1 750,280 + Pf1N1B4_656 0.12 -1.3 750,280 + Pf1N1B4_656 0.12 -0.0 750,280 + Pf1N1B4_656 0.12 -1.3 750,280 + Pf1N1B4_656 0.12 -0.6 750,281 - Pf1N1B4_656 0.12 -2.8 750,281 - Pf1N1B4_656 0.12 -0.6 750,281 - Pf1N1B4_656 0.12 -0.4 750,281 - Pf1N1B4_656 0.12 -0.4 750,281 - Pf1N1B4_656 0.12 -0.0 750,281 - Pf1N1B4_656 0.12 -0.2 750,281 - Pf1N1B4_656 0.12 -0.6 750,330 + Pf1N1B4_656 0.16 -0.4 750,330 + +1.6 750,330 + Pf1N1B4_656 0.16 -0.4 750,330 + Pf1N1B4_656 0.16 +1.0 750,330 + Pf1N1B4_656 0.16 -1.0 750,331 - Pf1N1B4_656 0.16 +0.6 750,331 - Pf1N1B4_656 0.16 +0.5 750,331 - Pf1N1B4_656 0.16 -0.7 750,331 - Pf1N1B4_656 0.16 -1.0 750,331 - Pf1N1B4_656 0.16 -1.1 750,775 + Pf1N1B4_656 0.53 -0.8 750,775 + Pf1N1B4_656 0.53 -0.6 750,775 + Pf1N1B4_656 0.53 -0.3 750,775 + Pf1N1B4_656 0.53 -0.3 750,775 + Pf1N1B4_656 0.53 -0.1 750,775 + Pf1N1B4_656 0.53 -0.6 750,776 - Pf1N1B4_656 0.53 -0.0 750,842 + Pf1N1B4_656 0.58 -1.3 750,843 - Pf1N1B4_656 0.58 -1.2 750,843 - Pf1N1B4_656 0.58 -1.1 750,843 - Pf1N1B4_656 0.58 -0.0 750,843 - Pf1N1B4_656 0.58 -2.0 750,843 - Pf1N1B4_656 0.58 +0.6 751,016 - -0.0 751,138 + Pf1N1B4_656 0.82 +0.3 751,139 - Pf1N1B4_656 0.82 -0.9 751,143 + Pf1N1B4_656 0.83 -0.2 751,365 + -1.2 751,366 - +0.6 751,372 + -0.5 751,372 + -0.3 751,372 + -0.8 751,372 + +1.0 751,373 - +0.2 751,373 - -0.3 751,373 - -1.7 751,401 + -1.1 751,401 + +0.1 751,401 + -1.4 751,401 + -1.5 751,402 - +0.8 751,419 + -3.4 751,419 + -0.9 751,419 + +0.3 751,419 + +0.4 751,419 + -0.0 751,444 + +1.3 751,445 - +1.0 751,445 - +0.1 751,445 - +1.6 751,474 + +1.0 751,475 - +0.4 751,475 - +0.2 751,475 - -0.7 751,487 + -0.6
Or see this region's nucleotide sequence