Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_617 and Pf1N1B4_618 are separated by 12 nucleotides Pf1N1B4_618 and Pf1N1B4_619 are separated by 216 nucleotides Pf1N1B4_619 and Pf1N1B4_620 are separated by 457 nucleotides Pf1N1B4_620 and Pf1N1B4_621 are separated by 11 nucleotides
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Pf1N1B4_618 - Acetyl-CoA carboxylase alpha subunit, at 714,686 to 715,048
_618
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Pf1N1B4_620 - Glycine cleavage system H protein, at 717,231 to 717,614
_620
Pf1N1B4_621 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 717,626 to 720,478
_621
Position (kb)
715
716
717 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.660 kb on + strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.752 kb on + strand at 714.752 kb on + strand, within Pf1N1B4_618 at 714.752 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand at 714.770 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.807 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.920 kb on + strand, within Pf1N1B4_618 at 714.975 kb on + strand, within Pf1N1B4_618 at 715.073 kb on + strand at 715.227 kb on + strand at 715.227 kb on + strand at 715.228 kb on - strand at 715.228 kb on - strand at 715.379 kb on + strand at 715.380 kb on - strand at 715.772 kb on + strand at 715.867 kb on + strand, within Pf1N1B4_619 at 716.134 kb on - strand, within Pf1N1B4_619 at 716.396 kb on + strand, within Pf1N1B4_619 at 716.397 kb on - strand, within Pf1N1B4_619 at 716.460 kb on - strand at 716.472 kb on + strand, within Pf1N1B4_619 at 716.670 kb on - strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.750 kb on + strand at 716.751 kb on - strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.843 kb on - strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.847 kb on - strand at 716.858 kb on + strand at 716.859 kb on - strand at 716.871 kb on + strand at 716.871 kb on + strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 717.072 kb on - strand at 717.221 kb on - strand at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.580 kb on - strand at 717.637 kb on + strand at 717.637 kb on + strand at 717.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 714,315 - Pf1N1B4_617 0.61 +1.0 714,315 - Pf1N1B4_617 0.61 +0.4 714,660 + -0.1 714,661 - +2.0 714,661 - -1.8 714,661 - +1.0 714,661 - -0.6 714,665 + +0.7 714,665 + -0.3 714,665 + -1.0 714,665 + -0.0 714,665 + -0.5 714,665 + +0.7 714,665 + +0.6 714,665 + +0.2 714,665 + +1.0 714,665 + -0.4 714,665 + -0.0 714,665 + -2.6 714,665 + +0.8 714,665 + +0.1 714,665 + +0.4 714,665 + +0.2 714,665 + +1.0 714,666 - -1.5 714,666 - +1.6 714,666 - +1.0 714,666 - +0.2 714,666 - +1.6 714,666 - -0.0 714,666 - -0.4 714,666 - -0.6 714,666 - +1.6 714,671 + +1.4 714,671 + -1.3 714,671 + -0.6 714,672 - -0.6 714,672 - -0.8 714,672 - -1.0 714,672 - -0.0 714,752 + +1.0 714,752 + Pf1N1B4_618 0.18 -0.0 714,752 + -0.0 714,770 + Pf1N1B4_618 0.23 +0.4 714,770 + Pf1N1B4_618 0.23 +0.8 714,770 + Pf1N1B4_618 0.23 +0.6 714,770 + +0.4 714,770 + +2.0 714,770 + Pf1N1B4_618 0.23 +1.0 714,771 - Pf1N1B4_618 0.23 -0.1 714,771 - +1.0 714,771 - Pf1N1B4_618 0.23 +1.0 714,771 - Pf1N1B4_618 0.23 +0.4 714,771 - Pf1N1B4_618 0.23 -2.7 714,771 - Pf1N1B4_618 0.23 -0.0 714,807 + Pf1N1B4_618 0.33 +1.2 714,881 + Pf1N1B4_618 0.54 -2.0 714,881 + Pf1N1B4_618 0.54 +1.0 714,881 + Pf1N1B4_618 0.54 +1.0 714,881 + Pf1N1B4_618 0.54 -0.6 714,881 + Pf1N1B4_618 0.54 +0.6 714,920 + Pf1N1B4_618 0.64 -0.3 714,975 + Pf1N1B4_618 0.80 -1.8 715,073 + -0.4 715,227 + +1.7 715,227 + +1.0 715,228 - +0.6 715,228 - -0.7 715,379 + -0.0 715,380 - -0.6 715,772 + -0.3 715,867 + Pf1N1B4_619 0.40 -0.0 716,134 - Pf1N1B4_619 0.58 -1.0 716,396 + Pf1N1B4_619 0.75 -1.8 716,397 - Pf1N1B4_619 0.75 -1.0 716,460 - -0.0 716,472 + Pf1N1B4_619 0.80 -1.2 716,670 - -1.3 716,746 + +0.4 716,746 + +2.6 716,746 + -1.3 716,747 - -0.2 716,747 - -0.3 716,747 - -0.4 716,750 + -2.0 716,751 - -0.2 716,757 + +1.0 716,757 + -0.0 716,757 + +1.0 716,758 - -0.8 716,758 - -0.8 716,758 - -0.2 716,761 + +0.3 716,761 + -0.2 716,761 + +0.8 716,762 - -0.0 716,762 - -0.1 716,762 - -2.5 716,762 - +1.7 716,762 - +1.0 716,762 - -0.5 716,843 - +1.0 716,846 + +1.6 716,846 + +0.7 716,846 + -0.0 716,847 - +1.7 716,858 + +1.0 716,859 - +1.0 716,871 + -0.0 716,871 + -0.2 716,872 - +1.0 716,872 - -0.6 716,872 - -1.3 716,872 - +0.1 716,872 - +0.7 717,072 - +1.0 717,221 - -0.4 717,426 + Pf1N1B4_620 0.51 -0.8 717,426 + Pf1N1B4_620 0.51 -0.2 717,426 + Pf1N1B4_620 0.51 -0.0 717,426 + Pf1N1B4_620 0.51 +2.3 717,427 - Pf1N1B4_620 0.51 -1.0 717,427 - Pf1N1B4_620 0.51 +0.9 717,427 - Pf1N1B4_620 0.51 +1.0 717,427 - -0.0 717,427 - Pf1N1B4_620 0.51 +1.3 717,427 - Pf1N1B4_620 0.51 +0.4 717,427 - Pf1N1B4_620 0.51 -0.0 717,427 - +0.4 717,580 - -1.3 717,637 + +0.6 717,637 + -2.0 717,638 - +1.2
Or see this region's nucleotide sequence