Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6037 and Pf1N1B4_6038 are separated by 213 nucleotides Pf1N1B4_6038 and Pf1N1B4_6039 are separated by 4 nucleotides Pf1N1B4_6039 and Pf1N1B4_6040 are separated by 74 nucleotides
Pf1N1B4_6037 - L-asparaginase (EC 3.5.1.1), at 501,468 to 502,556
_6037
Pf1N1B4_6038 - FIG00953874: hypothetical protein, at 502,770 to 503,009
_6038
Pf1N1B4_6039 - Endonuclease I precursor (EC 3.1.21.1), at 503,014 to 503,703
_6039
Pf1N1B4_6040 - FIG00956858: hypothetical protein, at 503,778 to 504,143
_6040
Position (kb) 502
503
504 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 501.864 kb on + strand, within Pf1N1B4_6037 at 501.865 kb on - strand at 501.865 kb on - strand, within Pf1N1B4_6037 at 501.911 kb on - strand, within Pf1N1B4_6037 at 502.230 kb on + strand, within Pf1N1B4_6037 at 502.255 kb on - strand at 502.366 kb on + strand, within Pf1N1B4_6037 at 502.366 kb on + strand, within Pf1N1B4_6037 at 502.408 kb on + strand at 502.440 kb on + strand, within Pf1N1B4_6037 at 502.440 kb on + strand, within Pf1N1B4_6037 at 502.441 kb on - strand, within Pf1N1B4_6037 at 502.551 kb on + strand at 502.551 kb on + strand at 502.551 kb on + strand at 502.552 kb on - strand at 502.660 kb on - strand at 502.705 kb on - strand at 502.734 kb on + strand at 502.764 kb on - strand at 502.796 kb on + strand at 502.796 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 503.036 kb on + strand at 503.119 kb on + strand, within Pf1N1B4_6039 at 503.144 kb on - strand at 503.158 kb on + strand, within Pf1N1B4_6039 at 503.158 kb on + strand, within Pf1N1B4_6039 at 503.159 kb on - strand at 503.195 kb on - strand at 503.195 kb on - strand, within Pf1N1B4_6039 at 503.329 kb on + strand, within Pf1N1B4_6039 at 503.330 kb on - strand, within Pf1N1B4_6039 at 503.330 kb on - strand at 503.437 kb on + strand, within Pf1N1B4_6039 at 503.521 kb on + strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.527 kb on + strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.535 kb on + strand, within Pf1N1B4_6039 at 503.535 kb on + strand, within Pf1N1B4_6039 at 503.578 kb on + strand, within Pf1N1B4_6039 at 503.578 kb on + strand, within Pf1N1B4_6039 at 503.579 kb on - strand at 503.579 kb on - strand, within Pf1N1B4_6039 at 503.592 kb on + strand, within Pf1N1B4_6039 at 503.599 kb on + strand, within Pf1N1B4_6039 at 503.600 kb on - strand, within Pf1N1B4_6039 at 503.889 kb on - strand, within Pf1N1B4_6040 at 503.889 kb on - strand, within Pf1N1B4_6040 at 503.948 kb on + strand at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 501,864 + Pf1N1B4_6037 0.36 +0.5 501,865 - +0.5 501,865 - Pf1N1B4_6037 0.36 -0.3 501,911 - Pf1N1B4_6037 0.41 +1.2 502,230 + Pf1N1B4_6037 0.70 +0.5 502,255 - -0.5 502,366 + Pf1N1B4_6037 0.82 -2.1 502,366 + Pf1N1B4_6037 0.82 -0.5 502,408 + +0.5 502,440 + Pf1N1B4_6037 0.89 -0.5 502,440 + Pf1N1B4_6037 0.89 -0.8 502,441 - Pf1N1B4_6037 0.89 -1.5 502,551 + -0.3 502,551 + +1.5 502,551 + -0.2 502,552 - -1.7 502,660 - +1.5 502,705 - +0.1 502,734 + -0.7 502,764 - -1.7 502,796 + -0.8 502,796 + Pf1N1B4_6038 0.11 +0.1 502,800 + Pf1N1B4_6038 0.12 -1.8 502,800 + Pf1N1B4_6038 0.12 -1.3 502,800 + Pf1N1B4_6038 0.12 -0.9 502,801 - Pf1N1B4_6038 0.13 -0.4 502,801 - Pf1N1B4_6038 0.13 -1.5 502,801 - Pf1N1B4_6038 0.13 -0.3 502,801 - Pf1N1B4_6038 0.13 -0.3 503,036 + -1.9 503,119 + Pf1N1B4_6039 0.15 +0.5 503,144 - +2.1 503,158 + Pf1N1B4_6039 0.21 +0.1 503,158 + Pf1N1B4_6039 0.21 -0.3 503,159 - +0.5 503,195 - +0.5 503,195 - Pf1N1B4_6039 0.26 +0.2 503,329 + Pf1N1B4_6039 0.46 +0.1 503,330 - Pf1N1B4_6039 0.46 +1.1 503,330 - -0.1 503,437 + Pf1N1B4_6039 0.61 +0.4 503,521 + Pf1N1B4_6039 0.73 -0.9 503,522 - Pf1N1B4_6039 0.74 -1.3 503,522 - Pf1N1B4_6039 0.74 -0.8 503,522 - Pf1N1B4_6039 0.74 -0.9 503,522 - Pf1N1B4_6039 0.74 -2.8 503,527 + Pf1N1B4_6039 0.74 +1.0 503,528 - Pf1N1B4_6039 0.74 +2.5 503,528 - Pf1N1B4_6039 0.74 -0.2 503,528 - Pf1N1B4_6039 0.74 +0.2 503,535 + Pf1N1B4_6039 0.76 -1.1 503,535 + Pf1N1B4_6039 0.76 -1.1 503,578 + Pf1N1B4_6039 0.82 -0.5 503,578 + Pf1N1B4_6039 0.82 -1.2 503,579 - -0.2 503,579 - Pf1N1B4_6039 0.82 -2.2 503,592 + Pf1N1B4_6039 0.84 -2.5 503,599 + Pf1N1B4_6039 0.85 +0.7 503,600 - Pf1N1B4_6039 0.85 -0.2 503,889 - Pf1N1B4_6040 0.30 +1.1 503,889 - Pf1N1B4_6040 0.30 +1.4 503,948 + -2.3 503,949 - Pf1N1B4_6040 0.47 +0.5 503,949 - -0.5 503,949 - Pf1N1B4_6040 0.47 -0.5 503,949 - Pf1N1B4_6040 0.47 -0.9 503,949 - Pf1N1B4_6040 0.47 -0.5 503,949 - Pf1N1B4_6040 0.47 +0.0 503,949 - Pf1N1B4_6040 0.47 -0.5 503,949 - Pf1N1B4_6040 0.47 +1.8 503,949 - Pf1N1B4_6040 0.47 -1.6 503,949 - Pf1N1B4_6040 0.47 +1.1
Or see this region's nucleotide sequence