Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5851 and Pf1N1B4_5852 are separated by 2 nucleotides Pf1N1B4_5852 and Pf1N1B4_5853 overlap by 4 nucleotides Pf1N1B4_5853 and Pf1N1B4_5854 overlap by 17 nucleotides Pf1N1B4_5854 and Pf1N1B4_5855 are separated by 57 nucleotides
Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611
_5851
Pf1N1B4_5852 - Amidases related to nicotinamidase, at 284,614 to 285,258
_5852
Pf1N1B4_5853 - Glutamine synthetase (EC 6.3.1.2), putative, at 285,255 to 286,586
_5853
Pf1N1B4_5854 - FIG00960144: hypothetical protein, at 286,570 to 287,322
_5854
Pf1N1B4_5855 - Putrescine importer, at 287,380 to 288,729
_5855
Position (kb)
285
286
287 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 284.402 kb on + strand, within Pf1N1B4_5851 at 284.403 kb on - strand at 284.609 kb on + strand at 284.610 kb on - strand at 285.154 kb on + strand, within Pf1N1B4_5852 at 285.155 kb on - strand at 285.172 kb on + strand, within Pf1N1B4_5852 at 285.444 kb on + strand, within Pf1N1B4_5853 at 286.005 kb on + strand, within Pf1N1B4_5853 at 286.005 kb on + strand, within Pf1N1B4_5853 at 286.071 kb on + strand, within Pf1N1B4_5853 at 286.116 kb on + strand, within Pf1N1B4_5853 at 286.116 kb on + strand, within Pf1N1B4_5853 at 286.240 kb on - strand, within Pf1N1B4_5853 at 286.570 kb on - strand at 286.607 kb on - strand at 286.624 kb on - strand at 286.819 kb on + strand at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.819 kb on + strand, within Pf1N1B4_5854 at 286.820 kb on - strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.051 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.053 kb on + strand, within Pf1N1B4_5854 at 287.092 kb on + strand, within Pf1N1B4_5854 at 287.093 kb on - strand, within Pf1N1B4_5854 at 287.173 kb on + strand, within Pf1N1B4_5854 at 287.302 kb on + strand at 287.320 kb on + strand at 287.328 kb on + strand at 287.329 kb on - strand at 287.329 kb on - strand at 287.387 kb on + strand at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.536 kb on + strand, within Pf1N1B4_5855 at 287.537 kb on - strand, within Pf1N1B4_5855
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 284,402 + Pf1N1B4_5851 0.75 -1.3 284,403 - +1.5 284,609 + +0.8 284,610 - +0.5 285,154 + Pf1N1B4_5852 0.84 -2.5 285,155 - +0.1 285,172 + Pf1N1B4_5852 0.87 +0.5 285,444 + Pf1N1B4_5853 0.14 -2.7 286,005 + Pf1N1B4_5853 0.56 -0.5 286,005 + Pf1N1B4_5853 0.56 -1.1 286,071 + Pf1N1B4_5853 0.61 -0.1 286,116 + Pf1N1B4_5853 0.65 -0.6 286,116 + Pf1N1B4_5853 0.65 +0.1 286,240 - Pf1N1B4_5853 0.74 -0.5 286,570 - +0.0 286,607 - +0.8 286,624 - +0.5 286,819 + +1.1 286,819 + Pf1N1B4_5854 0.33 +1.1 286,819 + Pf1N1B4_5854 0.33 +0.7 286,820 - Pf1N1B4_5854 0.33 -0.1 287,051 + Pf1N1B4_5854 0.64 +0.5 287,051 + Pf1N1B4_5854 0.64 -0.9 287,053 + Pf1N1B4_5854 0.64 -0.3 287,053 + Pf1N1B4_5854 0.64 -0.2 287,053 + Pf1N1B4_5854 0.64 +0.5 287,053 + Pf1N1B4_5854 0.64 +1.0 287,053 + Pf1N1B4_5854 0.64 +0.2 287,092 + Pf1N1B4_5854 0.69 -1.2 287,093 - Pf1N1B4_5854 0.69 +0.1 287,173 + Pf1N1B4_5854 0.80 +0.2 287,302 + +1.5 287,320 + -0.8 287,328 + -0.7 287,329 - +2.4 287,329 - -1.6 287,387 + +0.3 287,536 + Pf1N1B4_5855 0.12 -1.2 287,536 + Pf1N1B4_5855 0.12 -1.3 287,537 - Pf1N1B4_5855 0.12 -0.1
Or see this region's nucleotide sequence