Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5767 and Pf1N1B4_5768 are separated by 1 nucleotides Pf1N1B4_5768 and Pf1N1B4_5769 overlap by 4 nucleotides Pf1N1B4_5769 and Pf1N1B4_5770 overlap by 8 nucleotides Pf1N1B4_5770 and Pf1N1B4_5771 overlap by 26 nucleotides
Pf1N1B4_5767 - Heme d1 biosynthesis protein NirF, at 205,503 to 206,681
_5767
Pf1N1B4_5768 - Heme d1 biosynthesis protein NirD, at 206,683 to 207,141
_5768
Pf1N1B4_5769 - Heme d1 biosynthesis protein NirL, at 207,138 to 207,653
_5769
Pf1N1B4_5770 - Heme d1 biosynthesis protein NirG, at 207,646 to 208,089
_5770
Pf1N1B4_5771 - Heme d1 biosynthesis protein NirH, at 208,064 to 208,579
_5771
Position (kb) 207
208 Strain fitness (log2 ratio) -2
-1
0
1
2 at 206.756 kb on - strand, within Pf1N1B4_5768 at 206.756 kb on - strand, within Pf1N1B4_5768 at 206.964 kb on + strand, within Pf1N1B4_5768 at 206.965 kb on - strand, within Pf1N1B4_5768 at 206.968 kb on + strand at 206.968 kb on + strand, within Pf1N1B4_5768 at 206.969 kb on - strand, within Pf1N1B4_5768 at 206.987 kb on - strand, within Pf1N1B4_5768 at 207.344 kb on + strand, within Pf1N1B4_5769 at 207.344 kb on + strand, within Pf1N1B4_5769 at 207.439 kb on - strand, within Pf1N1B4_5769 at 207.472 kb on - strand, within Pf1N1B4_5769 at 207.929 kb on - strand, within Pf1N1B4_5770 at 207.929 kb on - strand, within Pf1N1B4_5770 at 208.060 kb on + strand at 208.060 kb on + strand at 208.061 kb on - strand at 208.076 kb on - strand at 208.284 kb on - strand, within Pf1N1B4_5771 at 208.548 kb on + strand at 208.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 206,756 - Pf1N1B4_5768 0.16 -2.1 206,756 - Pf1N1B4_5768 0.16 +0.1 206,964 + Pf1N1B4_5768 0.61 -1.3 206,965 - Pf1N1B4_5768 0.61 -2.4 206,968 + -1.5 206,968 + Pf1N1B4_5768 0.62 -0.5 206,969 - Pf1N1B4_5768 0.62 +0.8 206,987 - Pf1N1B4_5768 0.66 -0.5 207,344 + Pf1N1B4_5769 0.40 +1.3 207,344 + Pf1N1B4_5769 0.40 -0.5 207,439 - Pf1N1B4_5769 0.58 -1.9 207,472 - Pf1N1B4_5769 0.65 -1.1 207,929 - Pf1N1B4_5770 0.64 -0.5 207,929 - Pf1N1B4_5770 0.64 +1.8 208,060 + -0.6 208,060 + -0.2 208,061 - +0.2 208,076 - +0.2 208,284 - Pf1N1B4_5771 0.43 -0.3 208,548 + +0.6 208,618 + -0.5
Or see this region's nucleotide sequence