Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5541 and Pf1N1B4_5542 are separated by 497 nucleotides Pf1N1B4_5542 and Pf1N1B4_5543 are separated by 370 nucleotides Pf1N1B4_5543 and Pf1N1B4_5544 are separated by 10 nucleotides
Pf1N1B4_5541 - hypothetical protein, at 307,334 to 307,822
_5541
Pf1N1B4_5542 - hypothetical protein, at 308,320 to 308,523
_5542
Pf1N1B4_5543 - hypothetical protein, at 308,894 to 309,010
_5543
Pf1N1B4_5544 - Methyl-accepting chemotaxis protein, at 309,021 to 311,015
_5544
Position (kb) 308
309 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 307.343 kb on + strand at 307.344 kb on - strand at 307.460 kb on + strand, within Pf1N1B4_5541 at 307.472 kb on + strand, within Pf1N1B4_5541 at 307.472 kb on + strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.473 kb on - strand, within Pf1N1B4_5541 at 307.587 kb on + strand at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.587 kb on + strand, within Pf1N1B4_5541 at 307.588 kb on - strand, within Pf1N1B4_5541 at 307.588 kb on - strand, within Pf1N1B4_5541 at 307.588 kb on - strand, within Pf1N1B4_5541 at 307.656 kb on + strand, within Pf1N1B4_5541 at 307.656 kb on + strand, within Pf1N1B4_5541 at 307.657 kb on - strand, within Pf1N1B4_5541 at 307.745 kb on + strand, within Pf1N1B4_5541 at 307.746 kb on - strand, within Pf1N1B4_5541 at 307.800 kb on - strand at 307.840 kb on + strand at 307.840 kb on + strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.841 kb on - strand at 307.965 kb on - strand at 308.061 kb on - strand at 308.113 kb on + strand at 308.114 kb on - strand at 308.114 kb on - strand at 308.117 kb on + strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.122 kb on - strand at 308.122 kb on - strand at 308.129 kb on - strand at 308.129 kb on - strand at 308.152 kb on - strand at 308.152 kb on - strand at 308.152 kb on - strand at 308.252 kb on - strand at 308.366 kb on - strand, within Pf1N1B4_5542 at 308.366 kb on - strand, within Pf1N1B4_5542 at 308.449 kb on + strand, within Pf1N1B4_5542 at 308.450 kb on - strand, within Pf1N1B4_5542 at 308.521 kb on + strand at 308.522 kb on - strand at 308.522 kb on - strand at 308.574 kb on - strand at 308.574 kb on - strand at 308.637 kb on - strand at 308.695 kb on - strand at 308.695 kb on - strand at 308.702 kb on + strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.848 kb on + strand at 308.848 kb on + strand at 308.848 kb on + strand at 308.849 kb on - strand at 308.849 kb on - strand at 308.849 kb on - strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.963 kb on + strand, within Pf1N1B4_5543 at 308.964 kb on - strand, within Pf1N1B4_5543 at 308.964 kb on - strand at 308.993 kb on - strand, within Pf1N1B4_5543 at 309.004 kb on - strand at 309.004 kb on - strand at 309.004 kb on - strand at 309.004 kb on - strand at 309.036 kb on + strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.181 kb on - strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.183 kb on - strand at 309.183 kb on - strand at 309.213 kb on + strand at 309.213 kb on + strand at 309.214 kb on - strand at 309.248 kb on - strand, within Pf1N1B4_5544 at 309.330 kb on - strand, within Pf1N1B4_5544 at 309.344 kb on + strand, within Pf1N1B4_5544 at 309.359 kb on - strand at 309.359 kb on - strand at 309.398 kb on + strand, within Pf1N1B4_5544 at 309.398 kb on + strand, within Pf1N1B4_5544 at 309.410 kb on + strand, within Pf1N1B4_5544 at 309.421 kb on + strand, within Pf1N1B4_5544 at 309.421 kb on + strand, within Pf1N1B4_5544 at 309.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 307,343 + +0.9 307,344 - +0.3 307,460 + Pf1N1B4_5541 0.26 +0.0 307,472 + Pf1N1B4_5541 0.28 +1.0 307,472 + Pf1N1B4_5541 0.28 +0.7 307,473 - Pf1N1B4_5541 0.28 +0.4 307,473 - Pf1N1B4_5541 0.28 -0.1 307,473 - Pf1N1B4_5541 0.28 -0.1 307,473 - Pf1N1B4_5541 0.28 +0.0 307,473 - Pf1N1B4_5541 0.28 -0.3 307,587 + +1.6 307,587 + Pf1N1B4_5541 0.52 -0.3 307,587 + Pf1N1B4_5541 0.52 -1.1 307,587 + Pf1N1B4_5541 0.52 +0.4 307,587 + Pf1N1B4_5541 0.52 -0.8 307,587 + Pf1N1B4_5541 0.52 -1.8 307,587 + Pf1N1B4_5541 0.52 -1.5 307,587 + Pf1N1B4_5541 0.52 -0.3 307,588 - Pf1N1B4_5541 0.52 +1.0 307,588 - Pf1N1B4_5541 0.52 +1.2 307,588 - Pf1N1B4_5541 0.52 -0.2 307,656 + Pf1N1B4_5541 0.66 -1.3 307,656 + Pf1N1B4_5541 0.66 +1.0 307,657 - Pf1N1B4_5541 0.66 +0.4 307,745 + Pf1N1B4_5541 0.84 +1.0 307,746 - Pf1N1B4_5541 0.84 +0.0 307,800 - -1.6 307,840 + -1.0 307,840 + +0.0 307,841 - +0.4 307,841 - +0.4 307,841 - +1.1 307,841 - +0.0 307,841 - +0.1 307,965 - -0.0 308,061 - +0.7 308,113 + -1.0 308,114 - -1.0 308,114 - +0.8 308,117 + -0.3 308,118 - -1.0 308,118 - -2.1 308,118 - +1.6 308,118 - -0.6 308,118 - +0.4 308,118 - -1.1 308,118 - -0.3 308,122 - +1.0 308,122 - +0.8 308,129 - +0.7 308,129 - +0.6 308,152 - +0.8 308,152 - -0.8 308,152 - -1.1 308,252 - -1.8 308,366 - Pf1N1B4_5542 0.23 +1.0 308,366 - Pf1N1B4_5542 0.23 -1.3 308,449 + Pf1N1B4_5542 0.63 +0.4 308,450 - Pf1N1B4_5542 0.64 +0.0 308,521 + +1.0 308,522 - -1.8 308,522 - +0.7 308,574 - +1.0 308,574 - +0.2 308,637 - +1.0 308,695 - -1.7 308,695 - +1.4 308,702 + +2.0 308,762 - +1.0 308,762 - +1.0 308,762 - -0.1 308,762 - -0.3 308,848 + -0.3 308,848 + -1.3 308,848 + +1.0 308,849 - +0.3 308,849 - +0.2 308,849 - -0.6 308,850 + +0.7 308,850 + +2.0 308,850 + +0.3 308,850 + -0.4 308,851 - +0.2 308,851 - -0.6 308,851 - +0.1 308,851 - -1.6 308,851 - +0.2 308,851 - +0.0 308,963 + Pf1N1B4_5543 0.59 +0.5 308,964 - Pf1N1B4_5543 0.60 +0.3 308,964 - +1.0 308,993 - Pf1N1B4_5543 0.85 +0.0 309,004 - +1.9 309,004 - +0.6 309,004 - -1.3 309,004 - +0.0 309,036 + +2.4 309,037 - -0.3 309,037 - +1.4 309,037 - +3.6 309,037 - -0.6 309,037 - -0.3 309,037 - -0.8 309,180 + +0.0 309,180 + +0.0 309,180 + -1.0 309,181 - -0.1 309,182 + +0.4 309,182 + -0.2 309,182 + -0.6 309,182 + -1.0 309,183 - -0.2 309,183 - -0.6 309,213 + -0.4 309,213 + -0.4 309,214 - +1.0 309,248 - Pf1N1B4_5544 0.11 -0.3 309,330 - Pf1N1B4_5544 0.15 +0.0 309,344 + Pf1N1B4_5544 0.16 +0.8 309,359 - +0.0 309,359 - +0.0 309,398 + Pf1N1B4_5544 0.19 +0.3 309,398 + Pf1N1B4_5544 0.19 +0.6 309,410 + Pf1N1B4_5544 0.19 +0.2 309,421 + Pf1N1B4_5544 0.20 -0.6 309,421 + Pf1N1B4_5544 0.20 -1.0 309,422 - +1.0
Or see this region's nucleotide sequence