Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5499 and Pf1N1B4_5500 are separated by 177 nucleotides Pf1N1B4_5500 and Pf1N1B4_5501 are separated by 209 nucleotides Pf1N1B4_5501 and Pf1N1B4_5502 overlap by 35 nucleotides
Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695
_5499
Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862
_5500
Pf1N1B4_5501 - hypothetical protein, at 251,072 to 251,413
_5501
Pf1N1B4_5502 - hypothetical protein, at 251,379 to 252,542
_5502
Position (kb)
249
250
251 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 248.900 kb on + strand, within Pf1N1B4_5499 at 248.971 kb on + strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 249.004 kb on - strand at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.071 kb on - strand, within Pf1N1B4_5499 at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.232 kb on + strand at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.233 kb on - strand, within Pf1N1B4_5499 at 249.233 kb on - strand at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.274 kb on + strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.560 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.572 kb on - strand at 249.572 kb on - strand at 249.623 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.628 kb on - strand at 249.682 kb on + strand at 249.683 kb on - strand at 249.683 kb on - strand at 249.742 kb on - strand at 249.840 kb on - strand at 249.840 kb on - strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.882 kb on + strand at 249.883 kb on - strand at 249.883 kb on - strand at 249.883 kb on - strand at 250.144 kb on + strand, within Pf1N1B4_5500 at 250.145 kb on - strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.268 kb on - strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.737 kb on - strand, within Pf1N1B4_5500 at 250.757 kb on - strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.771 kb on - strand at 250.805 kb on - strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.870 kb on + strand at 250.870 kb on + strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.977 kb on - strand at 250.977 kb on - strand at 251.024 kb on + strand at 251.024 kb on + strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.068 kb on - strand at 251.260 kb on + strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.279 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.411 kb on + strand at 251.411 kb on + strand at 251.412 kb on - strand at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.534 kb on + strand, within Pf1N1B4_5502 at 251.590 kb on + strand at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.740 kb on - strand, within Pf1N1B4_5502 at 251.798 kb on + strand, within Pf1N1B4_5502
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 248,900 + Pf1N1B4_5499 0.56 -0.3 248,971 + Pf1N1B4_5499 0.60 -1.0 248,972 - Pf1N1B4_5499 0.60 +0.8 248,972 - Pf1N1B4_5499 0.60 -1.1 248,972 - Pf1N1B4_5499 0.60 -1.6 249,004 - -1.3 249,070 + Pf1N1B4_5499 0.65 +0.0 249,070 + Pf1N1B4_5499 0.65 +1.2 249,070 + Pf1N1B4_5499 0.65 +0.2 249,071 - Pf1N1B4_5499 0.65 +0.0 249,232 + Pf1N1B4_5499 0.74 -1.6 249,232 + +0.0 249,232 + Pf1N1B4_5499 0.74 -0.4 249,233 - Pf1N1B4_5499 0.74 -0.1 249,233 - +2.0 249,246 - Pf1N1B4_5499 0.75 -0.4 249,246 - Pf1N1B4_5499 0.75 -0.4 249,274 + Pf1N1B4_5499 0.77 +0.4 249,450 - Pf1N1B4_5499 0.86 -0.7 249,450 - Pf1N1B4_5499 0.86 +2.4 249,450 - Pf1N1B4_5499 0.86 +0.3 249,560 + +1.3 249,571 + -0.4 249,571 + +1.0 249,571 + +0.0 249,572 - +0.3 249,572 - +1.6 249,623 + +0.1 249,627 + +0.0 249,627 + -0.6 249,627 + -0.8 249,628 - -0.6 249,682 + +0.5 249,683 - +0.0 249,683 - +0.4 249,742 - -0.4 249,840 - -0.1 249,840 - +1.0 249,880 + -0.5 249,880 + +1.0 249,880 + -0.3 249,880 + +0.0 249,880 + +0.9 249,880 + +0.4 249,881 - +1.6 249,881 - +0.8 249,881 - -0.3 249,881 - -1.7 249,881 - -1.0 249,881 - +1.4 249,881 - +3.0 249,881 - +0.7 249,881 - +0.4 249,882 + -1.2 249,883 - +1.0 249,883 - +0.5 249,883 - +1.0 250,144 + Pf1N1B4_5500 0.27 +2.0 250,145 - Pf1N1B4_5500 0.27 -1.0 250,224 + Pf1N1B4_5500 0.35 -0.4 250,224 + +1.0 250,224 + Pf1N1B4_5500 0.35 +2.0 250,224 + -1.0 250,224 + Pf1N1B4_5500 0.35 +0.1 250,224 + Pf1N1B4_5500 0.35 +0.5 250,225 - Pf1N1B4_5500 0.36 -0.6 250,225 - Pf1N1B4_5500 0.36 -0.2 250,225 - Pf1N1B4_5500 0.36 +1.0 250,225 - Pf1N1B4_5500 0.36 -1.0 250,225 - Pf1N1B4_5500 0.36 +1.7 250,225 - Pf1N1B4_5500 0.36 +1.7 250,225 - Pf1N1B4_5500 0.36 +0.2 250,268 - Pf1N1B4_5500 0.40 +1.0 250,365 + Pf1N1B4_5500 0.50 +0.4 250,365 + Pf1N1B4_5500 0.50 +1.4 250,366 - Pf1N1B4_5500 0.50 -0.3 250,366 - Pf1N1B4_5500 0.50 +0.2 250,366 - Pf1N1B4_5500 0.50 -0.6 250,366 - Pf1N1B4_5500 0.50 +0.0 250,429 + Pf1N1B4_5500 0.56 +0.4 250,429 + Pf1N1B4_5500 0.56 -0.7 250,430 - Pf1N1B4_5500 0.56 +2.0 250,430 - Pf1N1B4_5500 0.56 +0.2 250,430 - Pf1N1B4_5500 0.56 +0.0 250,430 - Pf1N1B4_5500 0.56 -0.4 250,689 - Pf1N1B4_5500 0.82 -0.6 250,689 - Pf1N1B4_5500 0.82 +0.1 250,737 - Pf1N1B4_5500 0.87 -1.6 250,757 - -0.6 250,758 + Pf1N1B4_5500 0.89 -1.6 250,758 + -1.0 250,758 + Pf1N1B4_5500 0.89 +0.0 250,758 + Pf1N1B4_5500 0.89 +0.7 250,759 - Pf1N1B4_5500 0.89 +2.2 250,759 - Pf1N1B4_5500 0.89 -2.0 250,759 - Pf1N1B4_5500 0.89 -0.8 250,759 - Pf1N1B4_5500 0.89 +1.2 250,759 - Pf1N1B4_5500 0.89 +0.4 250,759 - Pf1N1B4_5500 0.89 +0.6 250,759 - Pf1N1B4_5500 0.89 -0.7 250,759 - Pf1N1B4_5500 0.89 -0.4 250,771 - -0.3 250,805 - -0.3 250,807 + -0.8 250,807 + +0.4 250,807 + -1.0 250,870 + +1.0 250,870 + +1.2 250,871 - +0.0 250,871 - -1.0 250,871 - +0.8 250,976 + -0.6 250,976 + -2.0 250,976 + -0.6 250,977 - +0.3 250,977 - +0.9 251,024 + +1.6 251,024 + +1.2 251,025 - +0.0 251,025 - +0.0 251,025 - -0.1 251,025 - -1.2 251,025 - -1.0 251,025 - +0.0 251,067 + +1.0 251,067 + -1.0 251,067 + -1.0 251,068 - -1.0 251,260 + Pf1N1B4_5501 0.55 -1.1 251,261 - Pf1N1B4_5501 0.55 +0.4 251,261 - Pf1N1B4_5501 0.55 +0.2 251,279 + Pf1N1B4_5501 0.61 -0.4 251,302 + Pf1N1B4_5501 0.67 +1.0 251,302 + Pf1N1B4_5501 0.67 -1.1 251,302 + Pf1N1B4_5501 0.67 -1.1 251,302 + Pf1N1B4_5501 0.67 -1.3 251,303 - Pf1N1B4_5501 0.68 +0.6 251,303 - Pf1N1B4_5501 0.68 +1.0 251,303 - Pf1N1B4_5501 0.68 -0.3 251,411 + +1.8 251,411 + +0.4 251,412 - -0.8 251,517 + Pf1N1B4_5502 0.12 -0.1 251,517 + Pf1N1B4_5502 0.12 +1.0 251,517 + Pf1N1B4_5502 0.12 +0.0 251,517 + Pf1N1B4_5502 0.12 +0.3 251,517 + Pf1N1B4_5502 0.12 +0.6 251,517 + Pf1N1B4_5502 0.12 +1.2 251,518 - Pf1N1B4_5502 0.12 +0.7 251,518 - Pf1N1B4_5502 0.12 -0.4 251,534 + Pf1N1B4_5502 0.13 -0.3 251,590 + -1.3 251,739 + Pf1N1B4_5502 0.31 -2.1 251,739 + Pf1N1B4_5502 0.31 +0.0 251,739 + Pf1N1B4_5502 0.31 +1.2 251,740 - Pf1N1B4_5502 0.31 -1.0 251,798 + Pf1N1B4_5502 0.36 -0.1
Or see this region's nucleotide sequence