Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_52 and Pf1N1B4_53 are separated by 6 nucleotides Pf1N1B4_53 and Pf1N1B4_54 are separated by 194 nucleotides Pf1N1B4_54 and Pf1N1B4_55 are separated by 237 nucleotides
Pf1N1B4_52 - FIG00956056: hypothetical protein, at 57,686 to 58,144
_52
Pf1N1B4_53 - hypothetical protein, at 58,151 to 58,342
_53
Pf1N1B4_54 - Cyclohexadienyl dehydratase (EC 4.2.1.51)(EC 4.2.1.91), at 58,537 to 59,328
_54
Pf1N1B4_55 - FIG00961388: hypothetical protein, at 59,566 to 59,892
_55
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 57.603 kb on - strand at 57.603 kb on - strand at 57.717 kb on - strand at 57.717 kb on - strand at 57.875 kb on - strand, within Pf1N1B4_52 at 58.055 kb on - strand, within Pf1N1B4_52 at 58.072 kb on + strand, within Pf1N1B4_52 at 58.093 kb on + strand, within Pf1N1B4_52 at 58.142 kb on + strand at 58.182 kb on + strand, within Pf1N1B4_53 at 58.186 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.207 kb on - strand, within Pf1N1B4_53 at 58.272 kb on + strand, within Pf1N1B4_53 at 58.352 kb on + strand at 58.388 kb on + strand at 58.389 kb on - strand at 58.389 kb on - strand at 58.426 kb on + strand at 58.427 kb on - strand at 58.427 kb on - strand at 58.548 kb on - strand at 58.684 kb on - strand, within Pf1N1B4_54 at 58.684 kb on - strand, within Pf1N1B4_54 at 58.684 kb on - strand, within Pf1N1B4_54 at 58.709 kb on - strand, within Pf1N1B4_54 at 59.132 kb on - strand at 59.145 kb on + strand, within Pf1N1B4_54 at 59.181 kb on + strand, within Pf1N1B4_54 at 59.182 kb on - strand, within Pf1N1B4_54 at 59.189 kb on - strand, within Pf1N1B4_54 at 59.203 kb on + strand, within Pf1N1B4_54 at 59.203 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.239 kb on + strand, within Pf1N1B4_54 at 59.282 kb on - strand at 59.282 kb on - strand at 59.322 kb on + strand at 59.322 kb on + strand at 59.326 kb on + strand at 59.326 kb on + strand at 59.326 kb on + strand at 59.350 kb on + strand at 59.350 kb on + strand at 59.504 kb on + strand at 59.504 kb on + strand at 59.504 kb on + strand at 59.505 kb on - strand at 59.505 kb on - strand at 59.505 kb on - strand at 59.505 kb on - strand at 59.549 kb on + strand at 59.550 kb on - strand at 59.603 kb on + strand, within Pf1N1B4_55 at 59.604 kb on - strand, within Pf1N1B4_55 at 59.604 kb on - strand, within Pf1N1B4_55 at 59.608 kb on - strand, within Pf1N1B4_55 at 59.608 kb on - strand, within Pf1N1B4_55 at 59.608 kb on - strand, within Pf1N1B4_55 at 59.608 kb on - strand, within Pf1N1B4_55 at 59.608 kb on - strand, within Pf1N1B4_55 at 59.608 kb on - strand, within Pf1N1B4_55 at 59.608 kb on - strand, within Pf1N1B4_55 at 59.663 kb on + strand, within Pf1N1B4_55 at 59.798 kb on + strand at 59.799 kb on - strand, within Pf1N1B4_55 at 59.799 kb on - strand, within Pf1N1B4_55 at 59.799 kb on - strand, within Pf1N1B4_55 at 59.811 kb on + strand, within Pf1N1B4_55 at 59.812 kb on - strand, within Pf1N1B4_55 at 59.851 kb on - strand, within Pf1N1B4_55 at 59.851 kb on - strand, within Pf1N1B4_55 at 59.895 kb on + strand at 59.903 kb on + strand at 59.904 kb on - strand at 59.995 kb on + strand at 60.096 kb on + strand at 60.096 kb on + strand at 60.096 kb on + strand at 60.096 kb on + strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.097 kb on - strand at 60.144 kb on + strand at 60.145 kb on - strand at 60.227 kb on - strand at 60.241 kb on + strand at 60.241 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 57,603 - +0.7 57,603 - -1.3 57,717 - +0.0 57,717 - -1.1 57,875 - Pf1N1B4_52 0.41 -1.3 58,055 - Pf1N1B4_52 0.80 +0.3 58,072 + Pf1N1B4_52 0.84 -0.6 58,093 + Pf1N1B4_52 0.89 +1.0 58,142 + -0.1 58,182 + Pf1N1B4_53 0.16 -1.1 58,186 + Pf1N1B4_53 0.18 -0.1 58,202 + Pf1N1B4_53 0.27 +0.0 58,202 + Pf1N1B4_53 0.27 +1.7 58,206 + +0.7 58,206 + Pf1N1B4_53 0.29 -0.3 58,206 + Pf1N1B4_53 0.29 +1.0 58,206 + Pf1N1B4_53 0.29 -1.3 58,206 + Pf1N1B4_53 0.29 -2.1 58,207 - Pf1N1B4_53 0.29 -0.1 58,272 + Pf1N1B4_53 0.63 +0.4 58,352 + +1.5 58,388 + -2.7 58,389 - +1.5 58,389 - +1.7 58,426 + -2.2 58,427 - +1.4 58,427 - -1.3 58,548 - +1.7 58,684 - Pf1N1B4_54 0.19 -0.1 58,684 - Pf1N1B4_54 0.19 +0.9 58,684 - Pf1N1B4_54 0.19 +0.5 58,709 - Pf1N1B4_54 0.22 +0.4 59,132 - +2.3 59,145 + Pf1N1B4_54 0.77 +0.7 59,181 + Pf1N1B4_54 0.81 +1.7 59,182 - Pf1N1B4_54 0.81 -0.8 59,189 - Pf1N1B4_54 0.82 +2.1 59,203 + Pf1N1B4_54 0.84 +0.2 59,203 + Pf1N1B4_54 0.84 +1.0 59,239 + Pf1N1B4_54 0.89 +0.0 59,239 + Pf1N1B4_54 0.89 +0.8 59,239 + Pf1N1B4_54 0.89 -0.1 59,239 + Pf1N1B4_54 0.89 -0.7 59,239 + +1.2 59,239 + Pf1N1B4_54 0.89 -0.1 59,239 + Pf1N1B4_54 0.89 -1.3 59,239 + Pf1N1B4_54 0.89 -0.3 59,282 - -2.1 59,282 - +1.5 59,322 + +0.9 59,322 + -0.5 59,326 + +0.7 59,326 + -0.3 59,326 + +2.7 59,350 + +0.7 59,350 + +0.7 59,504 + -0.6 59,504 + -0.8 59,504 + -0.3 59,505 - -0.6 59,505 - +3.1 59,505 - +1.7 59,505 - -0.3 59,549 + +0.2 59,550 - -0.5 59,603 + Pf1N1B4_55 0.11 +0.5 59,604 - Pf1N1B4_55 0.12 -0.6 59,604 - Pf1N1B4_55 0.12 -1.6 59,608 - Pf1N1B4_55 0.13 +1.2 59,608 - Pf1N1B4_55 0.13 -0.5 59,608 - Pf1N1B4_55 0.13 +1.3 59,608 - Pf1N1B4_55 0.13 +1.6 59,608 - Pf1N1B4_55 0.13 +1.3 59,608 - Pf1N1B4_55 0.13 -0.3 59,608 - Pf1N1B4_55 0.13 +0.3 59,663 + Pf1N1B4_55 0.30 +0.5 59,798 + +1.1 59,799 - Pf1N1B4_55 0.71 +1.2 59,799 - Pf1N1B4_55 0.71 +0.1 59,799 - Pf1N1B4_55 0.71 +0.9 59,811 + Pf1N1B4_55 0.75 -0.4 59,812 - Pf1N1B4_55 0.75 +1.3 59,851 - Pf1N1B4_55 0.87 +0.2 59,851 - Pf1N1B4_55 0.87 -1.1 59,895 + -0.3 59,903 + -0.6 59,904 - +2.1 59,995 + -0.5 60,096 + +0.1 60,096 + +0.4 60,096 + +2.7 60,096 + +0.4 60,097 - -0.3 60,097 - -0.1 60,097 - -0.6 60,097 - +0.9 60,097 - +1.7 60,144 + +0.2 60,145 - +2.0 60,227 - -1.8 60,241 + +0.7 60,241 + +0.8
Or see this region's nucleotide sequence