Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5149 and Pf1N1B4_5150 are separated by 2 nucleotides
Pf1N1B4_5149 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 5,732,960 to 5,733,703
_5149
Pf1N1B4_5150 - FIG00933687: hypothetical protein, at 5,733,706 to 5,736,129
_5150
Position (kb)
5732
5733
5734 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5731.991 kb on + strand at 5731.992 kb on - strand at 5732.181 kb on + strand at 5732.193 kb on + strand at 5732.221 kb on + strand at 5732.221 kb on + strand at 5732.221 kb on + strand at 5732.272 kb on + strand at 5732.272 kb on + strand at 5732.336 kb on + strand at 5732.336 kb on + strand at 5732.338 kb on + strand at 5732.338 kb on + strand at 5732.338 kb on + strand at 5732.339 kb on - strand at 5732.348 kb on - strand at 5732.504 kb on - strand at 5732.504 kb on - strand at 5732.504 kb on - strand at 5732.504 kb on - strand at 5732.504 kb on - strand at 5732.558 kb on - strand at 5732.634 kb on - strand at 5732.860 kb on + strand at 5732.886 kb on - strand at 5733.172 kb on + strand, within Pf1N1B4_5149 at 5733.172 kb on + strand, within Pf1N1B4_5149 at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.173 kb on - strand at 5733.173 kb on - strand, within Pf1N1B4_5149 at 5733.257 kb on + strand, within Pf1N1B4_5149 at 5733.257 kb on + strand, within Pf1N1B4_5149 at 5733.257 kb on + strand, within Pf1N1B4_5149 at 5733.258 kb on - strand, within Pf1N1B4_5149 at 5733.258 kb on - strand, within Pf1N1B4_5149 at 5733.497 kb on + strand, within Pf1N1B4_5149 at 5733.498 kb on - strand, within Pf1N1B4_5149 at 5733.579 kb on + strand, within Pf1N1B4_5149 at 5734.036 kb on + strand, within Pf1N1B4_5150 at 5734.036 kb on + strand, within Pf1N1B4_5150 at 5734.037 kb on - strand, within Pf1N1B4_5150 at 5734.037 kb on - strand, within Pf1N1B4_5150 at 5734.037 kb on - strand, within Pf1N1B4_5150 at 5734.124 kb on - strand, within Pf1N1B4_5150 at 5734.360 kb on + strand at 5734.694 kb on - strand, within Pf1N1B4_5150
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,731,991 + -1.4 5,731,992 - +0.4 5,732,181 + +0.4 5,732,193 + +2.2 5,732,221 + -1.8 5,732,221 + +0.7 5,732,221 + -1.2 5,732,272 + +2.0 5,732,272 + -0.8 5,732,336 + +0.1 5,732,336 + -0.3 5,732,338 + +0.2 5,732,338 + +1.2 5,732,338 + -0.1 5,732,339 - -0.6 5,732,348 - +0.4 5,732,504 - -0.4 5,732,504 - +0.4 5,732,504 - -2.9 5,732,504 - -2.2 5,732,504 - +1.4 5,732,558 - +0.0 5,732,634 - -0.3 5,732,860 + -0.8 5,732,886 - +0.1 5,733,172 + Pf1N1B4_5149 0.28 -0.6 5,733,172 + Pf1N1B4_5149 0.28 -1.2 5,733,173 - Pf1N1B4_5149 0.29 -0.9 5,733,173 - Pf1N1B4_5149 0.29 -1.0 5,733,173 - Pf1N1B4_5149 0.29 +1.4 5,733,173 - +1.4 5,733,173 - Pf1N1B4_5149 0.29 -1.0 5,733,257 + Pf1N1B4_5149 0.40 -0.6 5,733,257 + Pf1N1B4_5149 0.40 -0.6 5,733,257 + Pf1N1B4_5149 0.40 +1.2 5,733,258 - Pf1N1B4_5149 0.40 -1.0 5,733,258 - Pf1N1B4_5149 0.40 -0.6 5,733,497 + Pf1N1B4_5149 0.72 -1.0 5,733,498 - Pf1N1B4_5149 0.72 +0.8 5,733,579 + Pf1N1B4_5149 0.83 -1.9 5,734,036 + Pf1N1B4_5150 0.14 +0.1 5,734,036 + Pf1N1B4_5150 0.14 +0.0 5,734,037 - Pf1N1B4_5150 0.14 +0.2 5,734,037 - Pf1N1B4_5150 0.14 -0.3 5,734,037 - Pf1N1B4_5150 0.14 +0.3 5,734,124 - Pf1N1B4_5150 0.17 +0.8 5,734,360 + -0.9 5,734,694 - Pf1N1B4_5150 0.41 -0.4
Or see this region's nucleotide sequence