Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5133 and Pf1N1B4_5134 are separated by 13 nucleotides Pf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotides Pf1N1B4_5135 and Pf1N1B4_5136 are separated by 124 nucleotides
Pf1N1B4_5133 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 5,711,996 to 5,713,180
_5133
Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045
_5134
Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685
_5135
Pf1N1B4_5136 - Carboxynorspermidine dehydrogenase, at 5,714,810 to 5,716,045
_5136
Position (kb)
5714
5715 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5713.718 kb on + strand, within Pf1N1B4_5134 at 5713.936 kb on - strand, within Pf1N1B4_5134 at 5714.038 kb on - strand at 5714.168 kb on - strand, within Pf1N1B4_5135 at 5714.222 kb on - strand, within Pf1N1B4_5135 at 5714.222 kb on - strand, within Pf1N1B4_5135 at 5714.436 kb on - strand, within Pf1N1B4_5135 at 5714.438 kb on + strand, within Pf1N1B4_5135 at 5714.439 kb on - strand, within Pf1N1B4_5135 at 5714.439 kb on - strand, within Pf1N1B4_5135 at 5714.533 kb on + strand, within Pf1N1B4_5135 at 5714.616 kb on + strand, within Pf1N1B4_5135 at 5714.684 kb on - strand at 5714.771 kb on - strand at 5714.985 kb on + strand, within Pf1N1B4_5136 at 5714.986 kb on - strand, within Pf1N1B4_5136 at 5714.986 kb on - strand, within Pf1N1B4_5136 at 5715.042 kb on + strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.234 kb on - strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.487 kb on - strand, within Pf1N1B4_5136 at 5715.536 kb on - strand, within Pf1N1B4_5136 at 5715.546 kb on + strand, within Pf1N1B4_5136 at 5715.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,713,718 + Pf1N1B4_5134 0.62 +0.2 5,713,936 - Pf1N1B4_5134 0.87 -2.4 5,714,038 - +0.3 5,714,168 - Pf1N1B4_5135 0.20 +0.4 5,714,222 - Pf1N1B4_5135 0.28 -2.2 5,714,222 - Pf1N1B4_5135 0.28 +1.8 5,714,436 - Pf1N1B4_5135 0.61 +1.6 5,714,438 + Pf1N1B4_5135 0.62 -0.3 5,714,439 - Pf1N1B4_5135 0.62 +0.4 5,714,439 - Pf1N1B4_5135 0.62 +0.0 5,714,533 + Pf1N1B4_5135 0.76 -0.2 5,714,616 + Pf1N1B4_5135 0.89 +1.1 5,714,684 - -0.3 5,714,771 - -1.2 5,714,985 + Pf1N1B4_5136 0.14 -1.7 5,714,986 - Pf1N1B4_5136 0.14 -1.2 5,714,986 - Pf1N1B4_5136 0.14 +0.1 5,715,042 + Pf1N1B4_5136 0.19 +1.7 5,715,043 - Pf1N1B4_5136 0.19 -0.3 5,715,043 - Pf1N1B4_5136 0.19 -1.0 5,715,043 - Pf1N1B4_5136 0.19 +0.2 5,715,043 - Pf1N1B4_5136 0.19 -0.3 5,715,043 - Pf1N1B4_5136 0.19 -0.8 5,715,234 - Pf1N1B4_5136 0.34 -1.2 5,715,252 + Pf1N1B4_5136 0.36 -0.2 5,715,252 + Pf1N1B4_5136 0.36 +0.0 5,715,252 + Pf1N1B4_5136 0.36 -1.2 5,715,253 - Pf1N1B4_5136 0.36 -0.5 5,715,253 - Pf1N1B4_5136 0.36 -0.9 5,715,253 - +1.4 5,715,253 - Pf1N1B4_5136 0.36 -0.3 5,715,253 - Pf1N1B4_5136 0.36 -0.6 5,715,253 - Pf1N1B4_5136 0.36 +0.0 5,715,340 - Pf1N1B4_5136 0.43 -0.2 5,715,340 - Pf1N1B4_5136 0.43 -0.6 5,715,487 - Pf1N1B4_5136 0.55 -2.9 5,715,536 - Pf1N1B4_5136 0.59 -0.9 5,715,546 + Pf1N1B4_5136 0.60 -0.3 5,715,657 + -0.3
Or see this region's nucleotide sequence