Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5021 and Pf1N1B4_5022 are separated by 127 nucleotides Pf1N1B4_5022 and Pf1N1B4_5023 overlap by 11 nucleotides Pf1N1B4_5023 and Pf1N1B4_5024 are separated by 60 nucleotides
Pf1N1B4_5021 - FIG00958580: hypothetical protein, at 5,569,700 to 5,569,876
_5021
Pf1N1B4_5022 - hypothetical protein, at 5,570,004 to 5,570,714
_5022
Pf1N1B4_5023 - Alkylated DNA repair protein AlkB, at 5,570,704 to 5,571,384
_5023
Pf1N1B4_5024 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,571,445 to 5,572,545
_5024
Position (kb) 5570
5571
5572 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5569.725 kb on - strand at 5569.725 kb on - strand, within Pf1N1B4_5021 at 5570.009 kb on + strand at 5570.122 kb on + strand at 5570.122 kb on + strand, within Pf1N1B4_5022 at 5570.134 kb on - strand, within Pf1N1B4_5022 at 5570.199 kb on + strand, within Pf1N1B4_5022 at 5570.199 kb on + strand, within Pf1N1B4_5022 at 5570.200 kb on - strand, within Pf1N1B4_5022 at 5570.200 kb on - strand, within Pf1N1B4_5022 at 5570.206 kb on - strand at 5570.206 kb on - strand, within Pf1N1B4_5022 at 5570.289 kb on + strand, within Pf1N1B4_5022 at 5570.289 kb on + strand at 5570.394 kb on + strand, within Pf1N1B4_5022 at 5570.409 kb on + strand, within Pf1N1B4_5022 at 5570.410 kb on - strand, within Pf1N1B4_5022 at 5570.800 kb on + strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.907 kb on + strand, within Pf1N1B4_5023 at 5570.908 kb on - strand at 5570.908 kb on - strand, within Pf1N1B4_5023 at 5570.974 kb on + strand, within Pf1N1B4_5023 at 5571.108 kb on + strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.418 kb on - strand at 5571.418 kb on - strand at 5571.437 kb on + strand at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.589 kb on + strand, within Pf1N1B4_5024 at 5571.590 kb on - strand at 5571.590 kb on - strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.947 kb on - strand, within Pf1N1B4_5024 at 5571.979 kb on + strand, within Pf1N1B4_5024 at 5571.979 kb on + strand, within Pf1N1B4_5024 at 5571.980 kb on - strand, within Pf1N1B4_5024 at 5572.280 kb on + strand, within Pf1N1B4_5024 at 5572.351 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.362 kb on - strand, within Pf1N1B4_5024
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,569,725 - +1.4 5,569,725 - Pf1N1B4_5021 0.14 +0.8 5,570,009 + +1.2 5,570,122 + +0.4 5,570,122 + Pf1N1B4_5022 0.17 -0.8 5,570,134 - Pf1N1B4_5022 0.18 -2.0 5,570,199 + Pf1N1B4_5022 0.27 -2.2 5,570,199 + Pf1N1B4_5022 0.27 -0.9 5,570,200 - Pf1N1B4_5022 0.28 -1.9 5,570,200 - Pf1N1B4_5022 0.28 -2.2 5,570,206 - +0.8 5,570,206 - Pf1N1B4_5022 0.28 -0.1 5,570,289 + Pf1N1B4_5022 0.40 +0.2 5,570,289 + -1.6 5,570,394 + Pf1N1B4_5022 0.55 -1.0 5,570,409 + Pf1N1B4_5022 0.57 -0.6 5,570,410 - Pf1N1B4_5022 0.57 +0.3 5,570,800 + Pf1N1B4_5023 0.14 +2.0 5,570,801 - Pf1N1B4_5023 0.14 +0.4 5,570,801 - Pf1N1B4_5023 0.14 +1.2 5,570,801 - Pf1N1B4_5023 0.14 -1.2 5,570,907 + Pf1N1B4_5023 0.30 +0.3 5,570,908 - +2.0 5,570,908 - Pf1N1B4_5023 0.30 +0.4 5,570,974 + Pf1N1B4_5023 0.40 -0.6 5,571,108 + Pf1N1B4_5023 0.59 -0.7 5,571,109 - Pf1N1B4_5023 0.59 -0.4 5,571,109 - -0.6 5,571,109 - Pf1N1B4_5023 0.59 +0.6 5,571,109 - Pf1N1B4_5023 0.59 +1.7 5,571,418 - +0.4 5,571,418 - -1.6 5,571,437 + -0.6 5,571,566 - Pf1N1B4_5024 0.11 +0.5 5,571,566 - Pf1N1B4_5024 0.11 -0.3 5,571,566 - Pf1N1B4_5024 0.11 +0.4 5,571,566 - Pf1N1B4_5024 0.11 -0.8 5,571,589 + Pf1N1B4_5024 0.13 -1.2 5,571,590 - +2.0 5,571,590 - Pf1N1B4_5024 0.13 -1.6 5,571,946 + Pf1N1B4_5024 0.46 +0.4 5,571,946 + Pf1N1B4_5024 0.46 +0.2 5,571,946 + Pf1N1B4_5024 0.46 -1.2 5,571,946 + Pf1N1B4_5024 0.46 -0.7 5,571,946 + Pf1N1B4_5024 0.46 +1.2 5,571,947 - Pf1N1B4_5024 0.46 -0.7 5,571,979 + Pf1N1B4_5024 0.49 -0.3 5,571,979 + Pf1N1B4_5024 0.49 -0.9 5,571,980 - Pf1N1B4_5024 0.49 +0.1 5,572,280 + Pf1N1B4_5024 0.76 +2.4 5,572,351 + Pf1N1B4_5024 0.82 +0.4 5,572,361 + Pf1N1B4_5024 0.83 +0.7 5,572,361 + Pf1N1B4_5024 0.83 +0.7 5,572,361 + Pf1N1B4_5024 0.83 +0.6 5,572,362 - Pf1N1B4_5024 0.83 -0.6
Or see this region's nucleotide sequence