Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4764 and Pf1N1B4_4765 are separated by 61 nucleotides Pf1N1B4_4765 and Pf1N1B4_4766 overlap by 8 nucleotides Pf1N1B4_4766 and Pf1N1B4_4767 are separated by 165 nucleotides
Pf1N1B4_4764 - 2-ketogluconate transporter, at 5,260,243 to 5,261,529
_4764
Pf1N1B4_4765 - 2-ketogluconate kinase (EC 2.7.1.13), at 5,261,591 to 5,262,610
_4765
Pf1N1B4_4766 - Epimerase KguE, at 5,262,603 to 5,263,385
_4766
Pf1N1B4_4767 - 2-ketogluconate utilization repressor PtxS, at 5,263,551 to 5,264,576
_4767
Position (kb)
5261
5262
5263 Strain fitness (log2 ratio)
-2
-1
0
1 at 5260.593 kb on - strand at 5260.593 kb on - strand, within Pf1N1B4_4764 at 5260.700 kb on + strand, within Pf1N1B4_4764 at 5260.981 kb on - strand, within Pf1N1B4_4764 at 5261.315 kb on + strand at 5261.315 kb on + strand at 5261.315 kb on + strand, within Pf1N1B4_4764 at 5261.316 kb on - strand, within Pf1N1B4_4764 at 5261.435 kb on + strand at 5261.436 kb on - strand at 5261.465 kb on + strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.529 kb on + strand at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.962 kb on - strand, within Pf1N1B4_4765 at 5262.291 kb on + strand at 5262.291 kb on + strand, within Pf1N1B4_4765 at 5262.701 kb on - strand, within Pf1N1B4_4766 at 5262.897 kb on - strand, within Pf1N1B4_4766 at 5263.190 kb on - strand at 5263.388 kb on + strand at 5263.389 kb on + strand at 5263.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,260,593 - -0.6 5,260,593 - Pf1N1B4_4764 0.27 -0.6 5,260,700 + Pf1N1B4_4764 0.36 +0.2 5,260,981 - Pf1N1B4_4764 0.57 -1.5 5,261,315 + -1.2 5,261,315 + +0.4 5,261,315 + Pf1N1B4_4764 0.83 +1.0 5,261,316 - Pf1N1B4_4764 0.83 -2.3 5,261,435 + -1.9 5,261,436 - -0.3 5,261,465 + -1.3 5,261,493 - +0.0 5,261,493 - +1.2 5,261,493 - +0.0 5,261,493 - +0.7 5,261,493 - +0.1 5,261,493 - +0.4 5,261,493 - -1.6 5,261,529 + +0.7 5,261,908 - Pf1N1B4_4765 0.31 +0.3 5,261,908 - Pf1N1B4_4765 0.31 -1.9 5,261,908 - Pf1N1B4_4765 0.31 -0.6 5,261,962 - Pf1N1B4_4765 0.36 -0.6 5,262,291 + +0.4 5,262,291 + Pf1N1B4_4765 0.69 -2.4 5,262,701 - Pf1N1B4_4766 0.13 -0.9 5,262,897 - Pf1N1B4_4766 0.38 -0.6 5,263,190 - +0.0 5,263,388 + +1.0 5,263,389 + -0.4 5,263,429 + +0.4
Or see this region's nucleotide sequence