Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4740 and Pf1N1B4_4741 are separated by 292 nucleotides Pf1N1B4_4741 and Pf1N1B4_4742 overlap by 4 nucleotides Pf1N1B4_4742 and Pf1N1B4_4743 are separated by 6 nucleotides Pf1N1B4_4743 and Pf1N1B4_4744 overlap by 4 nucleotides
Pf1N1B4_4740 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,236,816 to 5,238,450
_4740
Pf1N1B4_4741 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 5,238,743 to 5,239,573
_4741
Pf1N1B4_4742 - Cold-shock DNA-binding domain, at 5,239,570 to 5,240,001
_4742
Pf1N1B4_4743 - FIG00955061: hypothetical protein, at 5,240,008 to 5,240,367
_4743
Pf1N1B4_4744 - Transcriptional regulator, LysR family, at 5,240,364 to 5,240,957
_4744
Position (kb)
5238
5239
5240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5237.919 kb on - strand, within Pf1N1B4_4740 at 5237.919 kb on - strand, within Pf1N1B4_4740 at 5237.966 kb on + strand at 5237.966 kb on + strand, within Pf1N1B4_4740 at 5238.379 kb on + strand at 5238.379 kb on + strand at 5238.480 kb on + strand at 5238.541 kb on + strand at 5238.687 kb on - strand at 5238.687 kb on - strand at 5238.693 kb on + strand at 5238.693 kb on + strand at 5238.836 kb on - strand, within Pf1N1B4_4741 at 5238.883 kb on + strand, within Pf1N1B4_4741 at 5238.883 kb on + strand at 5238.886 kb on + strand, within Pf1N1B4_4741 at 5238.886 kb on + strand at 5239.072 kb on + strand, within Pf1N1B4_4741 at 5239.174 kb on + strand, within Pf1N1B4_4741 at 5239.174 kb on + strand, within Pf1N1B4_4741 at 5239.174 kb on + strand at 5239.346 kb on + strand, within Pf1N1B4_4741 at 5239.355 kb on + strand at 5239.459 kb on - strand, within Pf1N1B4_4741 at 5240.017 kb on + strand at 5240.017 kb on + strand at 5240.294 kb on + strand, within Pf1N1B4_4743 at 5240.295 kb on - strand, within Pf1N1B4_4743 at 5240.295 kb on - strand, within Pf1N1B4_4743 at 5240.499 kb on + strand, within Pf1N1B4_4744 at 5240.499 kb on + strand, within Pf1N1B4_4744 at 5240.500 kb on - strand, within Pf1N1B4_4744 at 5240.539 kb on + strand, within Pf1N1B4_4744 at 5240.539 kb on + strand, within Pf1N1B4_4744 at 5240.539 kb on + strand, within Pf1N1B4_4744 at 5240.558 kb on + strand, within Pf1N1B4_4744 at 5240.559 kb on - strand, within Pf1N1B4_4744 at 5240.559 kb on - strand, within Pf1N1B4_4744
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,237,919 - Pf1N1B4_4740 0.67 +0.4 5,237,919 - Pf1N1B4_4740 0.67 +0.4 5,237,966 + +1.4 5,237,966 + Pf1N1B4_4740 0.70 +1.7 5,238,379 + +1.2 5,238,379 + -0.6 5,238,480 + +0.4 5,238,541 + +0.1 5,238,687 - +0.7 5,238,687 - +1.0 5,238,693 + -0.8 5,238,693 + +1.0 5,238,836 - Pf1N1B4_4741 0.11 -1.4 5,238,883 + Pf1N1B4_4741 0.17 +0.4 5,238,883 + +1.4 5,238,886 + Pf1N1B4_4741 0.17 -1.9 5,238,886 + +1.4 5,239,072 + Pf1N1B4_4741 0.40 -0.3 5,239,174 + Pf1N1B4_4741 0.52 -1.4 5,239,174 + Pf1N1B4_4741 0.52 -0.5 5,239,174 + +0.4 5,239,346 + Pf1N1B4_4741 0.73 -0.3 5,239,355 + -0.2 5,239,459 - Pf1N1B4_4741 0.86 +0.7 5,240,017 + -0.6 5,240,017 + +0.4 5,240,294 + Pf1N1B4_4743 0.79 +0.0 5,240,295 - Pf1N1B4_4743 0.80 -1.6 5,240,295 - Pf1N1B4_4743 0.80 -1.2 5,240,499 + Pf1N1B4_4744 0.23 -0.2 5,240,499 + Pf1N1B4_4744 0.23 -1.6 5,240,500 - Pf1N1B4_4744 0.23 -0.3 5,240,539 + Pf1N1B4_4744 0.29 -0.6 5,240,539 + Pf1N1B4_4744 0.29 -0.2 5,240,539 + Pf1N1B4_4744 0.29 +1.2 5,240,558 + Pf1N1B4_4744 0.33 +0.0 5,240,559 - Pf1N1B4_4744 0.33 -2.8 5,240,559 - Pf1N1B4_4744 0.33 -0.9
Or see this region's nucleotide sequence