Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4593 and Pf1N1B4_4594 overlap by 4 nucleotides Pf1N1B4_4594 and Pf1N1B4_4595 are separated by 282 nucleotides
Pf1N1B4_4593 - ABC transporter, periplasmic substrate-binding protein, at 5,064,001 to 5,065,842
_4593
Pf1N1B4_4594 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 5,065,839 to 5,067,671
_4594
Pf1N1B4_4595 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,067,954 to 5,069,408
_4595
Position (kb)
5065
5066
5067
5068 Strain fitness (log2 ratio)
-2
-1
0
1 at 5064.983 kb on + strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5065.448 kb on - strand, within Pf1N1B4_4593 at 5065.487 kb on + strand, within Pf1N1B4_4593 at 5065.488 kb on - strand, within Pf1N1B4_4593 at 5065.589 kb on + strand, within Pf1N1B4_4593 at 5065.703 kb on - strand at 5065.942 kb on + strand at 5065.942 kb on + strand at 5065.942 kb on + strand at 5065.969 kb on + strand at 5065.970 kb on - strand at 5065.970 kb on - strand at 5066.218 kb on + strand, within Pf1N1B4_4594 at 5066.570 kb on - strand at 5066.588 kb on - strand, within Pf1N1B4_4594 at 5066.633 kb on + strand, within Pf1N1B4_4594 at 5066.894 kb on - strand, within Pf1N1B4_4594 at 5067.212 kb on - strand, within Pf1N1B4_4594 at 5067.223 kb on - strand, within Pf1N1B4_4594 at 5067.763 kb on + strand at 5068.067 kb on - strand at 5068.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,064,983 + Pf1N1B4_4593 0.53 -0.2 5,064,984 - Pf1N1B4_4593 0.53 -1.9 5,064,984 - Pf1N1B4_4593 0.53 +0.4 5,064,984 - Pf1N1B4_4593 0.53 +1.4 5,065,448 - Pf1N1B4_4593 0.79 -0.6 5,065,487 + Pf1N1B4_4593 0.81 +0.2 5,065,488 - Pf1N1B4_4593 0.81 +0.0 5,065,589 + Pf1N1B4_4593 0.86 +0.4 5,065,703 - +0.6 5,065,942 + +1.2 5,065,942 + -0.6 5,065,942 + +0.4 5,065,969 + -0.5 5,065,970 - +0.2 5,065,970 - +0.7 5,066,218 + Pf1N1B4_4594 0.21 -0.6 5,066,570 - +0.4 5,066,588 - Pf1N1B4_4594 0.41 -1.9 5,066,633 + Pf1N1B4_4594 0.43 -0.6 5,066,894 - Pf1N1B4_4594 0.58 +0.4 5,067,212 - Pf1N1B4_4594 0.75 -1.2 5,067,223 - Pf1N1B4_4594 0.76 +0.4 5,067,763 + +0.1 5,068,067 - +1.4 5,068,067 - +0.4
Or see this region's nucleotide sequence