Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4507 and Pf1N1B4_4508 overlap by 16 nucleotides Pf1N1B4_4508 and Pf1N1B4_4509 overlap by 4 nucleotides Pf1N1B4_4509 and Pf1N1B4_4510 are separated by 16 nucleotides Pf1N1B4_4510 and Pf1N1B4_4511 are separated by 45 nucleotides
Pf1N1B4_4507 - Sensory box histidine kinase/response regulator, at 4,969,873 to 4,971,558
_4507
Pf1N1B4_4508 - hypothetical protein, at 4,971,543 to 4,971,659
_4508
Pf1N1B4_4509 - Aldose 1-epimerase, at 4,971,656 to 4,972,417
_4509
Pf1N1B4_4510 - Gluconolactonase (EC 3.1.1.17), at 4,972,434 to 4,973,327
_4510
Pf1N1B4_4511 - D-galacturonate dehydrogenase (EC 1.1.1.203) (from data) , at 4,973,373 to 4,974,185
_4511
Position (kb)
4971
4972
4973 Strain fitness (log2 ratio)
-2
-1
0
1 at 4971.705 kb on + strand at 4971.708 kb on + strand at 4971.708 kb on + strand at 4971.709 kb on - strand at 4971.746 kb on + strand, within Pf1N1B4_4509 at 4971.746 kb on + strand, within Pf1N1B4_4509 at 4971.945 kb on + strand, within Pf1N1B4_4509 at 4971.945 kb on + strand, within Pf1N1B4_4509 at 4972.834 kb on + strand, within Pf1N1B4_4510 at 4972.834 kb on + strand, within Pf1N1B4_4510 at 4972.834 kb on + strand at 4973.249 kb on - strand at 4973.352 kb on + strand at 4973.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,971,705 + +0.3 4,971,708 + +0.5 4,971,708 + +0.7 4,971,709 - -1.5 4,971,746 + Pf1N1B4_4509 0.12 -0.2 4,971,746 + Pf1N1B4_4509 0.12 -0.5 4,971,945 + Pf1N1B4_4509 0.38 -1.1 4,971,945 + Pf1N1B4_4509 0.38 -1.0 4,972,834 + Pf1N1B4_4510 0.45 -1.9 4,972,834 + Pf1N1B4_4510 0.45 -0.9 4,972,834 + +0.5 4,973,249 - +1.5 4,973,352 + -0.5 4,973,417 - -0.3
Or see this region's nucleotide sequence