Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4270 and Pf1N1B4_4271 overlap by 8 nucleotides Pf1N1B4_4271 and Pf1N1B4_4272 are separated by 173 nucleotides Pf1N1B4_4272 and Pf1N1B4_4273 are separated by 74 nucleotides
Pf1N1B4_4270 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 4,733,645 to 4,735,054
_4270
Pf1N1B4_4271 - Hydrogen cyanide synthase HcnC / Opine oxidase subunit B, at 4,735,047 to 4,736,306
_4271
Pf1N1B4_4272 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 4,736,480 to 4,737,091
_4272
Pf1N1B4_4273 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 4,737,166 to 4,738,086
_4273
Position (kb)
4735
4736
4737 Strain fitness (log2 ratio)
-1
0
1
2 at 4734.085 kb on + strand, within Pf1N1B4_4270 at 4734.085 kb on + strand, within Pf1N1B4_4270 at 4734.183 kb on - strand, within Pf1N1B4_4270 at 4734.305 kb on + strand at 4734.305 kb on + strand, within Pf1N1B4_4270 at 4734.306 kb on - strand, within Pf1N1B4_4270 at 4734.306 kb on - strand, within Pf1N1B4_4270 at 4734.306 kb on - strand, within Pf1N1B4_4270 at 4734.306 kb on - strand, within Pf1N1B4_4270 at 4734.306 kb on - strand, within Pf1N1B4_4270 at 4734.368 kb on + strand, within Pf1N1B4_4270 at 4734.740 kb on + strand, within Pf1N1B4_4270 at 4735.052 kb on + strand at 4735.052 kb on + strand at 4735.053 kb on - strand at 4735.059 kb on + strand at 4735.059 kb on + strand at 4735.060 kb on - strand at 4735.107 kb on + strand at 4735.365 kb on + strand, within Pf1N1B4_4271 at 4735.365 kb on + strand at 4735.446 kb on + strand, within Pf1N1B4_4271 at 4735.669 kb on - strand, within Pf1N1B4_4271 at 4735.908 kb on + strand at 4736.027 kb on + strand, within Pf1N1B4_4271 at 4736.027 kb on + strand, within Pf1N1B4_4271 at 4736.028 kb on - strand, within Pf1N1B4_4271 at 4736.028 kb on - strand, within Pf1N1B4_4271 at 4736.256 kb on - strand at 4736.335 kb on + strand at 4736.353 kb on + strand at 4736.524 kb on - strand at 4736.524 kb on - strand at 4736.615 kb on - strand at 4737.060 kb on + strand at 4737.131 kb on + strand at 4737.132 kb on - strand at 4737.220 kb on - strand at 4737.220 kb on - strand at 4737.221 kb on + strand at 4737.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,734,085 + Pf1N1B4_4270 0.31 +0.0 4,734,085 + Pf1N1B4_4270 0.31 +0.6 4,734,183 - Pf1N1B4_4270 0.38 +0.3 4,734,305 + +1.6 4,734,305 + Pf1N1B4_4270 0.47 -1.2 4,734,306 - Pf1N1B4_4270 0.47 +0.4 4,734,306 - Pf1N1B4_4270 0.47 -0.8 4,734,306 - Pf1N1B4_4270 0.47 +2.2 4,734,306 - Pf1N1B4_4270 0.47 +0.1 4,734,306 - Pf1N1B4_4270 0.47 +0.3 4,734,368 + Pf1N1B4_4270 0.51 +0.8 4,734,740 + Pf1N1B4_4270 0.78 -0.2 4,735,052 + +0.3 4,735,052 + -0.7 4,735,053 - -0.4 4,735,059 + +0.6 4,735,059 + +0.6 4,735,060 - +1.0 4,735,107 + +1.1 4,735,365 + Pf1N1B4_4271 0.25 +0.6 4,735,365 + -1.4 4,735,446 + Pf1N1B4_4271 0.32 +1.1 4,735,669 - Pf1N1B4_4271 0.49 -0.1 4,735,908 + -0.4 4,736,027 + Pf1N1B4_4271 0.78 +0.6 4,736,027 + Pf1N1B4_4271 0.78 -0.6 4,736,028 - Pf1N1B4_4271 0.78 +0.8 4,736,028 - Pf1N1B4_4271 0.78 +1.4 4,736,256 - +1.2 4,736,335 + +1.6 4,736,353 + +1.3 4,736,524 - -1.0 4,736,524 - +2.6 4,736,615 - -1.0 4,737,060 + -1.4 4,737,131 + +0.8 4,737,132 - -0.6 4,737,220 - +1.6 4,737,220 - +1.0 4,737,221 + -1.2 4,737,222 - -0.1
Or see this region's nucleotide sequence