Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4165 and Pf1N1B4_4166 are separated by 130 nucleotides Pf1N1B4_4166 and Pf1N1B4_4167 are separated by 122 nucleotides Pf1N1B4_4167 and Pf1N1B4_4168 overlap by 1 nucleotides Pf1N1B4_4168 and Pf1N1B4_4169 are separated by 1 nucleotides
Pf1N1B4_4165 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 4,615,351 to 4,616,787
_4165
Pf1N1B4_4166 - Transcriptional regulator, LysR family, at 4,616,918 to 4,617,823
_4166
Pf1N1B4_4167 - hypothetical protein, at 4,617,946 to 4,618,296
_4167
Pf1N1B4_4168 - putative membrane protein, at 4,618,296 to 4,618,808
_4168
Pf1N1B4_4169 - Hydrolases of the alpha/beta superfamily, at 4,618,810 to 4,619,559
_4169
Position (kb)
4616
4617
4618 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4615.928 kb on - strand at 4616.093 kb on + strand, within Pf1N1B4_4165 at 4616.094 kb on - strand, within Pf1N1B4_4165 at 4616.097 kb on - strand at 4616.177 kb on - strand at 4616.194 kb on + strand, within Pf1N1B4_4165 at 4616.195 kb on - strand, within Pf1N1B4_4165 at 4616.195 kb on - strand, within Pf1N1B4_4165 at 4616.261 kb on + strand, within Pf1N1B4_4165 at 4616.261 kb on + strand, within Pf1N1B4_4165 at 4616.262 kb on - strand, within Pf1N1B4_4165 at 4616.762 kb on + strand at 4616.762 kb on + strand at 4616.763 kb on - strand at 4616.763 kb on - strand at 4616.767 kb on + strand at 4616.768 kb on - strand at 4616.768 kb on - strand at 4616.771 kb on + strand at 4616.771 kb on + strand at 4616.772 kb on - strand at 4616.772 kb on - strand at 4616.817 kb on + strand at 4616.817 kb on + strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4617.416 kb on + strand, within Pf1N1B4_4166 at 4617.416 kb on + strand, within Pf1N1B4_4166 at 4617.432 kb on - strand, within Pf1N1B4_4166 at 4617.432 kb on - strand, within Pf1N1B4_4166 at 4617.521 kb on + strand, within Pf1N1B4_4166 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.422 kb on - strand at 4618.422 kb on - strand at 4618.422 kb on - strand at 4618.422 kb on - strand, within Pf1N1B4_4168 at 4618.493 kb on + strand, within Pf1N1B4_4168 at 4618.800 kb on + strand at 4618.800 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,615,928 - +1.6 4,616,093 + Pf1N1B4_4165 0.52 +1.7 4,616,094 - Pf1N1B4_4165 0.52 -2.0 4,616,097 - +2.2 4,616,177 - +3.0 4,616,194 + Pf1N1B4_4165 0.59 +2.0 4,616,195 - Pf1N1B4_4165 0.59 +2.0 4,616,195 - Pf1N1B4_4165 0.59 -0.6 4,616,261 + Pf1N1B4_4165 0.63 +1.0 4,616,261 + Pf1N1B4_4165 0.63 -1.5 4,616,262 - Pf1N1B4_4165 0.63 +0.6 4,616,762 + -0.7 4,616,762 + -2.0 4,616,763 - -1.4 4,616,763 - -0.1 4,616,767 + +1.6 4,616,768 - +0.8 4,616,768 - -0.6 4,616,771 + +0.4 4,616,771 + -1.4 4,616,772 - -0.5 4,616,772 - -0.1 4,616,817 + -1.8 4,616,817 + -1.0 4,616,818 - +0.2 4,616,818 - -0.5 4,616,818 - +1.2 4,616,818 - +0.1 4,616,818 - -1.0 4,616,818 - -2.7 4,617,416 + Pf1N1B4_4166 0.55 -1.7 4,617,416 + Pf1N1B4_4166 0.55 -0.7 4,617,432 - Pf1N1B4_4166 0.57 -2.2 4,617,432 - Pf1N1B4_4166 0.57 +1.8 4,617,521 + Pf1N1B4_4166 0.67 -1.2 4,618,421 + Pf1N1B4_4168 0.24 +2.2 4,618,421 + Pf1N1B4_4168 0.24 +1.6 4,618,421 + Pf1N1B4_4168 0.24 -2.0 4,618,422 - -1.0 4,618,422 - +2.6 4,618,422 - +1.2 4,618,422 - Pf1N1B4_4168 0.25 -0.2 4,618,493 + Pf1N1B4_4168 0.38 -0.6 4,618,800 + -0.4 4,618,800 + -0.4
Or see this region's nucleotide sequence