Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4037 and Pf1N1B4_4038 overlap by 4 nucleotides Pf1N1B4_4038 and Pf1N1B4_4039 are separated by 2 nucleotides Pf1N1B4_4039 and Pf1N1B4_4040 are separated by 72 nucleotides Pf1N1B4_4040 and Pf1N1B4_4041 are separated by 10 nucleotides
Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740
_4037
Pf1N1B4_4038 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 4,489,737 to 4,490,234
_4038
Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021
_4039
Pf1N1B4_4040 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 4,492,094 to 4,492,768
_4040
Pf1N1B4_4041 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 4,492,779 to 4,493,192
_4041
Position (kb)
4490
4491
4492
4493 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4489.310 kb on + strand, within Pf1N1B4_4037 at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.745 kb on - strand at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.012 kb on - strand, within Pf1N1B4_4038 at 4490.273 kb on - strand at 4490.354 kb on - strand at 4490.404 kb on - strand at 4490.404 kb on - strand at 4490.563 kb on - strand, within Pf1N1B4_4039 at 4490.950 kb on - strand, within Pf1N1B4_4039 at 4490.998 kb on - strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.377 kb on + strand, within Pf1N1B4_4039 at 4491.377 kb on + strand, within Pf1N1B4_4039 at 4491.378 kb on - strand, within Pf1N1B4_4039 at 4491.378 kb on - strand, within Pf1N1B4_4039 at 4491.393 kb on + strand, within Pf1N1B4_4039 at 4491.393 kb on + strand, within Pf1N1B4_4039 at 4491.604 kb on - strand, within Pf1N1B4_4039 at 4491.628 kb on - strand, within Pf1N1B4_4039 at 4491.704 kb on + strand, within Pf1N1B4_4039 at 4491.704 kb on + strand, within Pf1N1B4_4039 at 4491.705 kb on - strand, within Pf1N1B4_4039 at 4491.713 kb on - strand, within Pf1N1B4_4039 at 4491.713 kb on - strand, within Pf1N1B4_4039 at 4491.716 kb on - strand, within Pf1N1B4_4039 at 4491.739 kb on - strand, within Pf1N1B4_4039 at 4491.816 kb on + strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.828 kb on + strand, within Pf1N1B4_4039 at 4491.829 kb on - strand, within Pf1N1B4_4039 at 4491.987 kb on + strand at 4491.988 kb on - strand at 4492.000 kb on - strand at 4492.000 kb on - strand at 4492.025 kb on + strand at 4492.025 kb on + strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.133 kb on - strand at 4492.296 kb on + strand, within Pf1N1B4_4040 at 4492.297 kb on - strand, within Pf1N1B4_4040 at 4492.350 kb on + strand, within Pf1N1B4_4040 at 4492.351 kb on - strand, within Pf1N1B4_4040 at 4492.396 kb on + strand at 4492.563 kb on + strand, within Pf1N1B4_4040 at 4492.585 kb on + strand, within Pf1N1B4_4040 at 4492.586 kb on - strand, within Pf1N1B4_4040 at 4492.761 kb on + strand at 4492.762 kb on - strand at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.879 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,489,310 + Pf1N1B4_4037 0.68 -0.9 4,489,741 - -1.9 4,489,741 - -2.1 4,489,741 - -0.3 4,489,745 - +2.3 4,490,011 + Pf1N1B4_4038 0.55 -1.1 4,490,011 + Pf1N1B4_4038 0.55 +0.0 4,490,012 - Pf1N1B4_4038 0.55 -0.6 4,490,273 - -0.8 4,490,354 - -0.3 4,490,404 - -1.9 4,490,404 - +0.1 4,490,563 - Pf1N1B4_4039 0.18 -2.6 4,490,950 - Pf1N1B4_4039 0.40 -0.2 4,490,998 - Pf1N1B4_4039 0.43 -0.6 4,491,060 + Pf1N1B4_4039 0.46 -1.3 4,491,060 + Pf1N1B4_4039 0.46 -0.5 4,491,060 + Pf1N1B4_4039 0.46 -1.4 4,491,061 - Pf1N1B4_4039 0.46 +0.0 4,491,061 - Pf1N1B4_4039 0.46 -2.6 4,491,061 - Pf1N1B4_4039 0.46 -0.5 4,491,061 - Pf1N1B4_4039 0.46 -0.1 4,491,377 + Pf1N1B4_4039 0.64 +0.3 4,491,377 + Pf1N1B4_4039 0.64 -0.3 4,491,378 - Pf1N1B4_4039 0.64 -0.9 4,491,378 - Pf1N1B4_4039 0.64 -2.6 4,491,393 + Pf1N1B4_4039 0.65 -1.1 4,491,393 + Pf1N1B4_4039 0.65 -0.6 4,491,604 - Pf1N1B4_4039 0.77 +0.1 4,491,628 - Pf1N1B4_4039 0.78 +0.3 4,491,704 + Pf1N1B4_4039 0.82 -0.3 4,491,704 + Pf1N1B4_4039 0.82 +0.7 4,491,705 - Pf1N1B4_4039 0.82 +0.7 4,491,713 - Pf1N1B4_4039 0.83 -0.3 4,491,713 - Pf1N1B4_4039 0.83 -1.1 4,491,716 - Pf1N1B4_4039 0.83 -0.3 4,491,739 - Pf1N1B4_4039 0.84 -2.5 4,491,816 + Pf1N1B4_4039 0.88 +0.1 4,491,817 - Pf1N1B4_4039 0.89 -0.8 4,491,817 - Pf1N1B4_4039 0.89 -1.1 4,491,817 - Pf1N1B4_4039 0.89 -1.9 4,491,828 + Pf1N1B4_4039 0.89 -1.4 4,491,829 - Pf1N1B4_4039 0.89 -0.6 4,491,987 + +0.5 4,491,988 - -1.3 4,492,000 - +0.3 4,492,000 - -1.4 4,492,025 + -0.6 4,492,025 + -2.6 4,492,026 - -0.5 4,492,026 - +0.7 4,492,026 - -0.3 4,492,026 - -1.3 4,492,048 - +0.1 4,492,048 - +0.7 4,492,048 - +0.1 4,492,048 - +0.4 4,492,079 - -0.5 4,492,079 - +0.7 4,492,079 - -0.3 4,492,079 - -1.3 4,492,079 - +0.1 4,492,133 - -1.3 4,492,296 + Pf1N1B4_4040 0.30 +1.4 4,492,297 - Pf1N1B4_4040 0.30 -2.1 4,492,350 + Pf1N1B4_4040 0.38 -0.3 4,492,351 - Pf1N1B4_4040 0.38 -2.5 4,492,396 + -0.3 4,492,563 + Pf1N1B4_4040 0.69 -1.1 4,492,585 + Pf1N1B4_4040 0.73 +0.9 4,492,586 - Pf1N1B4_4040 0.73 -0.5 4,492,761 + +0.2 4,492,762 - +0.7 4,492,853 - Pf1N1B4_4041 0.18 -1.4 4,492,853 - Pf1N1B4_4041 0.18 -1.8 4,492,853 - Pf1N1B4_4041 0.18 +0.7 4,492,879 - Pf1N1B4_4041 0.24 +0.0 4,492,994 - Pf1N1B4_4041 0.52 -0.3 4,492,994 - Pf1N1B4_4041 0.52 -0.3
Or see this region's nucleotide sequence