Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4034 and Pf1N1B4_4035 overlap by 4 nucleotides Pf1N1B4_4035 and Pf1N1B4_4036 overlap by 78 nucleotides Pf1N1B4_4036 and Pf1N1B4_4037 are separated by 328 nucleotides
Pf1N1B4_4034 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 4,484,527 to 4,485,534
_4034
Pf1N1B4_4035 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 4,485,531 to 4,487,990
_4035
Pf1N1B4_4036 - hypothetical protein, at 4,487,913 to 4,488,056
_4036
Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740
_4037
Position (kb)
4485
4486
4487
4488 Strain fitness (log2 ratio)
-2
-1
0
1 at 4484.807 kb on + strand, within Pf1N1B4_4034 at 4485.124 kb on - strand, within Pf1N1B4_4034 at 4485.503 kb on - strand at 4485.742 kb on + strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4486.005 kb on + strand, within Pf1N1B4_4035 at 4487.245 kb on + strand, within Pf1N1B4_4035 at 4487.246 kb on - strand, within Pf1N1B4_4035 at 4487.468 kb on - strand, within Pf1N1B4_4035 at 4487.707 kb on + strand, within Pf1N1B4_4035 at 4487.708 kb on - strand, within Pf1N1B4_4035 at 4487.756 kb on - strand at 4487.765 kb on - strand at 4488.152 kb on + strand at 4488.239 kb on - strand at 4488.330 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.471 kb on - strand at 4488.782 kb on + strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,484,807 + Pf1N1B4_4034 0.28 +0.1 4,485,124 - Pf1N1B4_4034 0.59 -2.4 4,485,503 - -0.3 4,485,742 + -1.1 4,485,743 - +0.7 4,485,743 - +0.3 4,485,743 - -0.3 4,485,743 - -0.2 4,486,005 + Pf1N1B4_4035 0.19 -0.7 4,487,245 + Pf1N1B4_4035 0.70 +0.2 4,487,246 - Pf1N1B4_4035 0.70 -2.1 4,487,468 - Pf1N1B4_4035 0.79 -1.6 4,487,707 + Pf1N1B4_4035 0.88 -0.3 4,487,708 - Pf1N1B4_4035 0.88 -1.3 4,487,756 - -0.2 4,487,765 - -1.8 4,488,152 + +0.1 4,488,239 - -0.3 4,488,330 - -0.3 4,488,379 - +1.1 4,488,379 - -0.5 4,488,379 - -0.7 4,488,471 - -0.5 4,488,782 + Pf1N1B4_4037 0.29 -0.3 4,488,783 - Pf1N1B4_4037 0.29 -0.3 4,488,783 - Pf1N1B4_4037 0.29 -1.1
Or see this region's nucleotide sequence