Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4018 and Pf1N1B4_4019 are separated by 49 nucleotides Pf1N1B4_4019 and Pf1N1B4_4020 are separated by 51 nucleotides
Pf1N1B4_4018 - Heavy metal RND efflux outer membrane protein, CzcC family, at 4,469,028 to 4,470,287
_4018
Pf1N1B4_4019 - Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family, at 4,470,337 to 4,471,530
_4019
Pf1N1B4_4020 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 4,471,582 to 4,474,083
_4020
Position (kb)
4470
4471
4472 Strain fitness (log2 ratio)
-1
0
1
2 at 4469.871 kb on + strand, within Pf1N1B4_4018 at 4469.872 kb on - strand, within Pf1N1B4_4018 at 4470.208 kb on - strand at 4470.799 kb on + strand, within Pf1N1B4_4019 at 4470.799 kb on + strand, within Pf1N1B4_4019 at 4470.800 kb on - strand, within Pf1N1B4_4019 at 4470.800 kb on - strand at 4470.920 kb on + strand, within Pf1N1B4_4019 at 4470.921 kb on - strand, within Pf1N1B4_4019 at 4470.921 kb on - strand, within Pf1N1B4_4019 at 4471.581 kb on + strand at 4471.581 kb on + strand at 4471.581 kb on + strand at 4471.582 kb on - strand at 4471.582 kb on - strand at 4471.673 kb on - strand at 4472.054 kb on - strand at 4472.054 kb on - strand, within Pf1N1B4_4020 at 4472.197 kb on + strand, within Pf1N1B4_4020
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,469,871 + Pf1N1B4_4018 0.67 +0.7 4,469,872 - Pf1N1B4_4018 0.67 +1.5 4,470,208 - +0.1 4,470,799 + Pf1N1B4_4019 0.39 +1.4 4,470,799 + Pf1N1B4_4019 0.39 +0.2 4,470,800 - Pf1N1B4_4019 0.39 -0.3 4,470,800 - -0.9 4,470,920 + Pf1N1B4_4019 0.49 +0.7 4,470,921 - Pf1N1B4_4019 0.49 +1.5 4,470,921 - Pf1N1B4_4019 0.49 -1.3 4,471,581 + +0.7 4,471,581 + +0.1 4,471,581 + -0.9 4,471,582 - +2.5 4,471,582 - -0.9 4,471,673 - -0.5 4,472,054 - -1.3 4,472,054 - Pf1N1B4_4020 0.19 -0.0 4,472,197 + Pf1N1B4_4020 0.25 -0.3
Or see this region's nucleotide sequence