Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_323 and Pf1N1B4_324 are separated by 567 nucleotides Pf1N1B4_324 and Pf1N1B4_325 are separated by 535 nucleotides
Pf1N1B4_323 - Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-), at 381,190 to 382,218
_323
Pf1N1B4_324 - Glycosyl transferase, group 2 family protein, at 382,786 to 383,091
_324
Pf1N1B4_325 - D amino acid oxidase (DAO) family (EC 1.4.3.3), at 383,627 to 384,769
_325
Position (kb) 382
383
384 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.373 kb on - strand at 382.391 kb on - strand at 382.413 kb on + strand at 382.413 kb on + strand at 382.414 kb on - strand at 382.414 kb on - strand at 382.418 kb on - strand at 382.418 kb on - strand at 382.426 kb on + strand at 382.583 kb on - strand at 382.583 kb on - strand at 382.590 kb on - strand at 382.646 kb on + strand at 382.646 kb on + strand at 382.647 kb on - strand at 382.647 kb on - strand at 382.647 kb on - strand at 382.647 kb on - strand at 382.653 kb on + strand at 382.654 kb on - strand at 382.707 kb on - strand at 382.740 kb on + strand at 382.741 kb on - strand at 382.741 kb on - strand at 382.741 kb on - strand at 382.877 kb on + strand, within Pf1N1B4_324 at 382.878 kb on - strand, within Pf1N1B4_324 at 383.009 kb on + strand, within Pf1N1B4_324 at 383.016 kb on + strand, within Pf1N1B4_324 at 383.174 kb on + strand at 383.175 kb on - strand at 383.175 kb on - strand at 383.175 kb on - strand at 383.178 kb on + strand at 383.179 kb on - strand at 383.322 kb on + strand at 383.323 kb on - strand at 383.448 kb on + strand at 383.448 kb on + strand at 383.449 kb on - strand at 383.449 kb on - strand at 383.491 kb on - strand at 383.546 kb on + strand at 383.624 kb on - strand at 383.624 kb on - strand at 383.627 kb on - strand at 383.627 kb on - strand at 383.627 kb on - strand at 383.627 kb on - strand at 383.660 kb on + strand at 383.756 kb on + strand, within Pf1N1B4_325 at 383.776 kb on - strand, within Pf1N1B4_325 at 383.881 kb on + strand, within Pf1N1B4_325 at 383.882 kb on - strand, within Pf1N1B4_325 at 383.884 kb on + strand, within Pf1N1B4_325 at 383.891 kb on + strand, within Pf1N1B4_325 at 384.042 kb on - strand, within Pf1N1B4_325 at 384.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 382,241 - +0.3 382,241 - +0.8 382,241 - +0.4 382,241 - +1.0 382,241 - +2.0 382,241 - +0.3 382,241 - -1.3 382,241 - +2.3 382,241 - +0.7 382,241 - +1.4 382,241 - -0.2 382,250 - +2.4 382,250 - +1.0 382,250 - -1.5 382,250 - -0.1 382,250 - +0.7 382,373 - +0.3 382,391 - -1.7 382,413 + +0.8 382,413 + +0.4 382,414 - -1.7 382,414 - +1.8 382,418 - +1.0 382,418 - -0.7 382,426 + +1.3 382,583 - +0.8 382,583 - +1.5 382,590 - +1.2 382,646 + +1.3 382,646 + +0.1 382,647 - -2.0 382,647 - +0.4 382,647 - +1.5 382,647 - +1.8 382,653 + -0.4 382,654 - +0.4 382,707 - -1.5 382,740 + +0.3 382,741 - -0.7 382,741 - -1.1 382,741 - +0.7 382,877 + Pf1N1B4_324 0.30 -0.3 382,878 - Pf1N1B4_324 0.30 -0.3 383,009 + Pf1N1B4_324 0.73 -0.2 383,016 + Pf1N1B4_324 0.75 -1.1 383,174 + -0.5 383,175 - -0.7 383,175 - +1.2 383,175 - -0.5 383,178 + +0.6 383,179 - -1.2 383,322 + +0.0 383,323 - -1.3 383,448 + +0.1 383,448 + -0.4 383,449 - -0.7 383,449 - +1.6 383,491 - +0.3 383,546 + -1.2 383,624 - +0.3 383,624 - -0.7 383,627 - +0.5 383,627 - +1.1 383,627 - +0.8 383,627 - +0.4 383,660 + -0.7 383,756 + Pf1N1B4_325 0.11 -2.1 383,776 - Pf1N1B4_325 0.13 -1.2 383,881 + Pf1N1B4_325 0.22 -0.4 383,882 - Pf1N1B4_325 0.22 -0.2 383,884 + Pf1N1B4_325 0.22 -2.2 383,891 + Pf1N1B4_325 0.23 +2.8 384,042 - Pf1N1B4_325 0.36 -0.2 384,053 - -0.2
Or see this region's nucleotide sequence