Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3168 and Pf1N1B4_3169 are separated by 44 nucleotides Pf1N1B4_3169 and Pf1N1B4_3170 are separated by 167 nucleotides
Pf1N1B4_3168 - AmpG permease, at 3,550,979 to 3,552,538
_3168
Pf1N1B4_3169 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 3,552,583 to 3,552,936
_3169
Pf1N1B4_3170 - Peptide chain release factor RF-3, at 3,553,104 to 3,554,111
_3170
Position (kb)
3552
3553 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3551.691 kb on + strand, within Pf1N1B4_3168 at 3551.692 kb on - strand, within Pf1N1B4_3168 at 3551.987 kb on - strand, within Pf1N1B4_3168 at 3551.988 kb on + strand, within Pf1N1B4_3168 at 3551.989 kb on - strand at 3551.989 kb on - strand, within Pf1N1B4_3168 at 3551.989 kb on - strand, within Pf1N1B4_3168 at 3552.075 kb on + strand, within Pf1N1B4_3168 at 3552.445 kb on - strand at 3552.624 kb on + strand, within Pf1N1B4_3169 at 3552.836 kb on + strand, within Pf1N1B4_3169 at 3552.837 kb on - strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.878 kb on + strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.879 kb on - strand, within Pf1N1B4_3169 at 3552.976 kb on + strand at 3552.981 kb on + strand at 3552.982 kb on - strand at 3552.982 kb on - strand at 3553.041 kb on + strand at 3553.042 kb on - strand at 3553.042 kb on - strand at 3553.103 kb on + strand at 3553.104 kb on - strand at 3553.104 kb on - strand at 3553.104 kb on - strand at 3553.213 kb on + strand, within Pf1N1B4_3170 at 3553.214 kb on - strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.329 kb on + strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.330 kb on - strand, within Pf1N1B4_3170 at 3553.468 kb on + strand, within Pf1N1B4_3170 at 3553.468 kb on + strand, within Pf1N1B4_3170 at 3553.469 kb on - strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.530 kb on + strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.531 kb on - strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.593 kb on + strand, within Pf1N1B4_3170 at 3553.594 kb on - strand, within Pf1N1B4_3170 at 3553.594 kb on - strand, within Pf1N1B4_3170 at 3553.594 kb on - strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.618 kb on + strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.619 kb on - strand, within Pf1N1B4_3170 at 3553.701 kb on + strand, within Pf1N1B4_3170 at 3553.701 kb on + strand, within Pf1N1B4_3170 at 3553.824 kb on + strand, within Pf1N1B4_3170 at 3553.824 kb on + strand, within Pf1N1B4_3170 at 3553.824 kb on + strand, within Pf1N1B4_3170 at 3553.825 kb on - strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.857 kb on + strand, within Pf1N1B4_3170 at 3553.881 kb on + strand, within Pf1N1B4_3170 at 3553.881 kb on + strand, within Pf1N1B4_3170 at 3553.882 kb on - strand, within Pf1N1B4_3170 at 3553.882 kb on - strand, within Pf1N1B4_3170 at 3553.882 kb on - strand at 3553.882 kb on - strand, within Pf1N1B4_3170
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,551,691 + Pf1N1B4_3168 0.46 -1.8 3,551,692 - Pf1N1B4_3168 0.46 -0.2 3,551,987 - Pf1N1B4_3168 0.65 -0.2 3,551,988 + Pf1N1B4_3168 0.65 -0.7 3,551,989 - +0.0 3,551,989 - Pf1N1B4_3168 0.65 -1.0 3,551,989 - Pf1N1B4_3168 0.65 -0.7 3,552,075 + Pf1N1B4_3168 0.70 -0.3 3,552,445 - +0.0 3,552,624 + Pf1N1B4_3169 0.12 +1.0 3,552,836 + Pf1N1B4_3169 0.71 +1.0 3,552,837 - Pf1N1B4_3169 0.72 +0.6 3,552,878 + Pf1N1B4_3169 0.83 +1.0 3,552,878 + Pf1N1B4_3169 0.83 +0.0 3,552,878 + Pf1N1B4_3169 0.83 +2.0 3,552,879 - Pf1N1B4_3169 0.84 +0.2 3,552,879 - Pf1N1B4_3169 0.84 +0.5 3,552,879 - Pf1N1B4_3169 0.84 +0.0 3,552,976 + +1.8 3,552,981 + +2.6 3,552,982 - +0.2 3,552,982 - +1.3 3,553,041 + -0.6 3,553,042 - -1.0 3,553,042 - -0.3 3,553,103 + +1.0 3,553,104 - +2.0 3,553,104 - +1.0 3,553,104 - -0.3 3,553,213 + Pf1N1B4_3170 0.11 +0.4 3,553,214 - Pf1N1B4_3170 0.11 -0.6 3,553,329 + Pf1N1B4_3170 0.22 +2.0 3,553,329 + Pf1N1B4_3170 0.22 +1.0 3,553,329 + Pf1N1B4_3170 0.22 -0.6 3,553,329 + Pf1N1B4_3170 0.22 -0.4 3,553,329 + Pf1N1B4_3170 0.22 +1.0 3,553,329 + Pf1N1B4_3170 0.22 +1.8 3,553,330 - Pf1N1B4_3170 0.22 -0.6 3,553,330 - Pf1N1B4_3170 0.22 -1.6 3,553,330 - Pf1N1B4_3170 0.22 -0.2 3,553,330 - Pf1N1B4_3170 0.22 +0.9 3,553,330 - Pf1N1B4_3170 0.22 -0.2 3,553,330 - Pf1N1B4_3170 0.22 +1.0 3,553,330 - Pf1N1B4_3170 0.22 +0.5 3,553,468 + Pf1N1B4_3170 0.36 +0.6 3,553,468 + Pf1N1B4_3170 0.36 +0.5 3,553,469 - Pf1N1B4_3170 0.36 -0.3 3,553,530 + Pf1N1B4_3170 0.42 +0.7 3,553,530 + Pf1N1B4_3170 0.42 +2.0 3,553,530 + Pf1N1B4_3170 0.42 +0.0 3,553,530 + Pf1N1B4_3170 0.42 +0.1 3,553,530 + Pf1N1B4_3170 0.42 +0.2 3,553,531 - Pf1N1B4_3170 0.42 +0.6 3,553,531 - Pf1N1B4_3170 0.42 -2.3 3,553,531 - Pf1N1B4_3170 0.42 +0.3 3,553,593 + Pf1N1B4_3170 0.49 -1.9 3,553,593 + Pf1N1B4_3170 0.49 +0.3 3,553,593 + Pf1N1B4_3170 0.49 +0.4 3,553,593 + Pf1N1B4_3170 0.49 +1.4 3,553,593 + Pf1N1B4_3170 0.49 +0.3 3,553,594 - Pf1N1B4_3170 0.49 +0.1 3,553,594 - Pf1N1B4_3170 0.49 +0.0 3,553,594 - Pf1N1B4_3170 0.49 -0.4 3,553,618 + Pf1N1B4_3170 0.51 +1.2 3,553,618 + Pf1N1B4_3170 0.51 -0.3 3,553,618 + Pf1N1B4_3170 0.51 -1.3 3,553,618 + Pf1N1B4_3170 0.51 -0.2 3,553,618 + Pf1N1B4_3170 0.51 -0.6 3,553,618 + Pf1N1B4_3170 0.51 -1.0 3,553,619 - Pf1N1B4_3170 0.51 +0.0 3,553,619 - Pf1N1B4_3170 0.51 -0.6 3,553,619 - -1.0 3,553,619 - Pf1N1B4_3170 0.51 +0.0 3,553,619 - Pf1N1B4_3170 0.51 +0.3 3,553,619 - Pf1N1B4_3170 0.51 +0.7 3,553,701 + Pf1N1B4_3170 0.59 +0.1 3,553,701 + Pf1N1B4_3170 0.59 -0.8 3,553,824 + Pf1N1B4_3170 0.71 +0.0 3,553,824 + Pf1N1B4_3170 0.71 +1.0 3,553,824 + Pf1N1B4_3170 0.71 +0.0 3,553,825 - Pf1N1B4_3170 0.72 -1.3 3,553,857 + Pf1N1B4_3170 0.75 -0.6 3,553,857 + Pf1N1B4_3170 0.75 +0.6 3,553,857 + Pf1N1B4_3170 0.75 -1.0 3,553,857 + Pf1N1B4_3170 0.75 +0.6 3,553,881 + Pf1N1B4_3170 0.77 +1.3 3,553,881 + Pf1N1B4_3170 0.77 -1.8 3,553,882 - Pf1N1B4_3170 0.77 +0.0 3,553,882 - Pf1N1B4_3170 0.77 +0.8 3,553,882 - +1.0 3,553,882 - Pf1N1B4_3170 0.77 +0.2
Or see this region's nucleotide sequence