Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3128 and Pf1N1B4_3129 are separated by 135 nucleotides Pf1N1B4_3129 and Pf1N1B4_3130 overlap by 1 nucleotides Pf1N1B4_3130 and Pf1N1B4_3131 overlap by 11 nucleotides
Pf1N1B4_3128 - FIG003269: Prophage tail protein, at 3,513,921 to 3,514,964
_3128
Pf1N1B4_3129 - Prophage baseplate assembly protein V, at 3,515,100 to 3,515,609
_3129
Pf1N1B4_3130 - Bacteriophage protein GP46, at 3,515,609 to 3,516,007
_3130
Pf1N1B4_3131 - Phage FluMu protein gp47, at 3,515,997 to 3,517,040
_3131
Position (kb)
3515
3516
3517 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3514.638 kb on + strand, within Pf1N1B4_3128 at 3514.638 kb on + strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.639 kb on - strand, within Pf1N1B4_3128 at 3514.755 kb on + strand, within Pf1N1B4_3128 at 3514.755 kb on + strand, within Pf1N1B4_3128 at 3514.755 kb on + strand, within Pf1N1B4_3128 at 3514.756 kb on - strand, within Pf1N1B4_3128 at 3514.756 kb on - strand, within Pf1N1B4_3128 at 3514.756 kb on - strand, within Pf1N1B4_3128 at 3514.872 kb on + strand at 3514.872 kb on + strand at 3514.873 kb on - strand at 3514.873 kb on - strand at 3514.873 kb on - strand at 3514.926 kb on - strand at 3515.030 kb on + strand at 3515.030 kb on + strand at 3515.030 kb on + strand at 3515.031 kb on - strand at 3515.038 kb on - strand at 3515.107 kb on + strand at 3515.108 kb on - strand at 3515.371 kb on - strand, within Pf1N1B4_3129 at 3515.371 kb on - strand, within Pf1N1B4_3129 at 3515.371 kb on - strand at 3515.543 kb on - strand, within Pf1N1B4_3129 at 3515.743 kb on + strand, within Pf1N1B4_3130 at 3515.831 kb on + strand, within Pf1N1B4_3130 at 3515.831 kb on + strand, within Pf1N1B4_3130 at 3515.832 kb on - strand, within Pf1N1B4_3130 at 3515.996 kb on + strand at 3515.996 kb on + strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3515.997 kb on - strand at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.325 kb on - strand, within Pf1N1B4_3131 at 3516.325 kb on - strand, within Pf1N1B4_3131 at 3516.465 kb on - strand, within Pf1N1B4_3131 at 3516.465 kb on - strand, within Pf1N1B4_3131 at 3516.585 kb on + strand, within Pf1N1B4_3131 at 3516.585 kb on + strand, within Pf1N1B4_3131 at 3516.586 kb on - strand, within Pf1N1B4_3131 at 3516.617 kb on - strand, within Pf1N1B4_3131 at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.669 kb on + strand at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.735 kb on + strand at 3516.735 kb on + strand, within Pf1N1B4_3131 at 3516.735 kb on + strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.804 kb on + strand, within Pf1N1B4_3131 at 3516.805 kb on - strand, within Pf1N1B4_3131 at 3516.805 kb on - strand, within Pf1N1B4_3131 at 3516.838 kb on + strand, within Pf1N1B4_3131
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,514,638 + Pf1N1B4_3128 0.69 +0.4 3,514,638 + Pf1N1B4_3128 0.69 +0.0 3,514,639 - Pf1N1B4_3128 0.69 -0.1 3,514,639 - Pf1N1B4_3128 0.69 +1.0 3,514,639 - Pf1N1B4_3128 0.69 +1.8 3,514,639 - Pf1N1B4_3128 0.69 -2.0 3,514,639 - Pf1N1B4_3128 0.69 +1.7 3,514,755 + Pf1N1B4_3128 0.80 +0.2 3,514,755 + Pf1N1B4_3128 0.80 -0.6 3,514,755 + Pf1N1B4_3128 0.80 +0.3 3,514,756 - Pf1N1B4_3128 0.80 +0.5 3,514,756 - Pf1N1B4_3128 0.80 -0.6 3,514,756 - Pf1N1B4_3128 0.80 +0.4 3,514,872 + +0.5 3,514,872 + +0.4 3,514,873 - -0.6 3,514,873 - +0.2 3,514,873 - -1.0 3,514,926 - +0.9 3,515,030 + -1.0 3,515,030 + -1.6 3,515,030 + +1.4 3,515,031 - -0.6 3,515,038 - -1.0 3,515,107 + +1.6 3,515,108 - -0.8 3,515,371 - Pf1N1B4_3129 0.53 -0.3 3,515,371 - Pf1N1B4_3129 0.53 +1.0 3,515,371 - +1.0 3,515,543 - Pf1N1B4_3129 0.87 -2.4 3,515,743 + Pf1N1B4_3130 0.34 -0.5 3,515,831 + Pf1N1B4_3130 0.56 -2.6 3,515,831 + Pf1N1B4_3130 0.56 -0.7 3,515,832 - Pf1N1B4_3130 0.56 -1.0 3,515,996 + -0.3 3,515,996 + +1.0 3,515,997 - +0.6 3,515,997 - -0.4 3,515,997 - -1.7 3,515,997 - +1.0 3,515,997 - +2.0 3,515,997 - -2.6 3,515,997 - -0.4 3,515,997 - +0.5 3,516,324 + Pf1N1B4_3131 0.31 -0.3 3,516,324 + Pf1N1B4_3131 0.31 +3.0 3,516,324 + Pf1N1B4_3131 0.31 -1.1 3,516,325 - Pf1N1B4_3131 0.31 +0.3 3,516,325 - Pf1N1B4_3131 0.31 +2.0 3,516,465 - Pf1N1B4_3131 0.45 +1.6 3,516,465 - Pf1N1B4_3131 0.45 -0.5 3,516,585 + Pf1N1B4_3131 0.56 +0.6 3,516,585 + Pf1N1B4_3131 0.56 +0.0 3,516,586 - Pf1N1B4_3131 0.56 -0.1 3,516,617 - Pf1N1B4_3131 0.59 +2.0 3,516,669 + Pf1N1B4_3131 0.64 +1.0 3,516,669 + +1.6 3,516,669 + Pf1N1B4_3131 0.64 -0.7 3,516,669 + Pf1N1B4_3131 0.64 +0.2 3,516,669 + Pf1N1B4_3131 0.64 +0.8 3,516,670 - Pf1N1B4_3131 0.64 -0.4 3,516,670 - Pf1N1B4_3131 0.64 -0.1 3,516,670 - Pf1N1B4_3131 0.64 -2.0 3,516,670 - Pf1N1B4_3131 0.64 +0.3 3,516,670 - Pf1N1B4_3131 0.64 +0.3 3,516,735 + +0.0 3,516,735 + Pf1N1B4_3131 0.71 -0.3 3,516,735 + Pf1N1B4_3131 0.71 +0.0 3,516,736 - Pf1N1B4_3131 0.71 -1.6 3,516,736 - Pf1N1B4_3131 0.71 +0.9 3,516,736 - Pf1N1B4_3131 0.71 +1.2 3,516,736 - Pf1N1B4_3131 0.71 +0.3 3,516,769 + Pf1N1B4_3131 0.74 +0.0 3,516,769 + Pf1N1B4_3131 0.74 -1.2 3,516,769 + Pf1N1B4_3131 0.74 -0.3 3,516,769 + Pf1N1B4_3131 0.74 +0.0 3,516,769 + +0.0 3,516,770 - Pf1N1B4_3131 0.74 +0.4 3,516,770 - Pf1N1B4_3131 0.74 +0.9 3,516,770 - Pf1N1B4_3131 0.74 +0.7 3,516,770 - Pf1N1B4_3131 0.74 -0.2 3,516,804 + Pf1N1B4_3131 0.77 +1.0 3,516,805 - Pf1N1B4_3131 0.77 +0.0 3,516,805 - Pf1N1B4_3131 0.77 -1.1 3,516,838 + Pf1N1B4_3131 0.81 -0.3
Or see this region's nucleotide sequence