Experiment: LB 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_3109 and Pf1N1B4_3110 are separated by 212 nucleotides Pf1N1B4_3110 and Pf1N1B4_3111 overlap by 4 nucleotides Pf1N1B4_3111 and Pf1N1B4_3112 overlap by 13 nucleotides Pf1N1B4_3112 and Pf1N1B4_3113 overlap by 1 nucleotides 
        Pf1N1B4_3109 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,496,179 to 3,497,024 
        _3109 
         
        
        Pf1N1B4_3110 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 3,497,237 to 3,497,710 
        _3110 
         
        
        Pf1N1B4_3111 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,497,707 to 3,498,765 
        _3111 
         
        
        Pf1N1B4_3112 - 5-nucleotidase SurE (EC 3.1.3.5) @ Exopolyphosphatase (EC 3.6.1.11), at 3,498,753 to 3,499,502 
        _3112 
         
        
        Pf1N1B4_3113 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 3,499,502 to 3,500,179 
        _3113 
         Position (kb) 3497 
3498 
3499 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.960 kb on - strand at 3497.014 kb on + strand at 3497.022 kb on + strand at 3497.181 kb on + strand at 3497.181 kb on + strand at 3497.182 kb on - strand at 3497.200 kb on + strand at 3497.200 kb on + strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.741 kb on - strand at 3497.811 kb on + strand at 3497.933 kb on - strand, within Pf1N1B4_3111 at 3498.177 kb on + strand, within Pf1N1B4_3111 at 3498.177 kb on + strand, within Pf1N1B4_3111 at 3498.178 kb on - strand, within Pf1N1B4_3111 at 3498.453 kb on + strand, within Pf1N1B4_3111 at 3498.758 kb on + strand at 3498.810 kb on + strand at 3498.810 kb on + strand at 3499.056 kb on + strand at 3499.056 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.454 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.523 kb on - strand at 3499.586 kb on + strand, within Pf1N1B4_3113 at 3499.586 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.708 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction LB remove 3,496,959 +  -0.2 3,496,959 +  -0.6 3,496,959 +  -0.3 3,496,959 +  -1.0 3,496,959 +  -1.1 3,496,959 +  -1.0 3,496,959 +  +0.5 3,496,959 +  -2.0 3,496,960 -  +0.0 3,497,014 +  -0.1 3,497,022 +  +0.8 3,497,181 +  -1.5 3,497,181 +  -1.4 3,497,182 -  +0.0 3,497,200 +  -0.4 3,497,200 +  -0.5 3,497,201 -  +1.6 3,497,201 -  -0.5 3,497,201 -  +2.0 3,497,741 -  +0.2 3,497,811 +  +0.0 3,497,933 -  Pf1N1B4_3111 0.21  +1.0 3,498,177 +  Pf1N1B4_3111 0.44  +0.0 3,498,177 +  Pf1N1B4_3111 0.44  +0.2 3,498,178 -  Pf1N1B4_3111 0.44  +1.0 3,498,453 +  Pf1N1B4_3111 0.70  +1.0 3,498,758 +  +2.0 3,498,810 +  +1.0 3,498,810 +  -0.7 3,499,056 +  -0.5 3,499,056 +  Pf1N1B4_3112 0.40  -1.8 3,499,138 +  Pf1N1B4_3112 0.51  -0.5 3,499,138 +  Pf1N1B4_3112 0.51  -2.0 3,499,138 +  Pf1N1B4_3112 0.51  -0.5 3,499,416 +  Pf1N1B4_3112 0.88  +0.0 3,499,416 +  Pf1N1B4_3112 0.88  -0.6 3,499,416 +  Pf1N1B4_3112 0.88  -1.0 3,499,416 +  Pf1N1B4_3112 0.88  +0.2 3,499,416 +  Pf1N1B4_3112 0.88  -1.8 3,499,416 +  Pf1N1B4_3112 0.88  +0.5 3,499,416 +  Pf1N1B4_3112 0.88  -1.1 3,499,416 +  Pf1N1B4_3112 0.88  +0.0 3,499,416 +  Pf1N1B4_3112 0.88  -1.0 3,499,416 +  Pf1N1B4_3112 0.88  -1.0 3,499,454 +  +0.4 3,499,522 +  +0.5 3,499,522 +  -1.6 3,499,522 +  -1.3 3,499,522 +  +0.5 3,499,522 +  -1.0 3,499,522 +  +0.0 3,499,523 -  -1.8 3,499,586 +  Pf1N1B4_3113 0.12  +2.6 3,499,586 +  Pf1N1B4_3113 0.12  +1.0 3,499,679 +  Pf1N1B4_3113 0.26  +1.0 3,499,679 +  Pf1N1B4_3113 0.26  -0.6 3,499,679 +  Pf1N1B4_3113 0.26  +0.7 3,499,687 +  Pf1N1B4_3113 0.27  +0.2 3,499,687 +  Pf1N1B4_3113 0.27  +0.1 3,499,687 +  Pf1N1B4_3113 0.27  +0.2 3,499,708 +  Pf1N1B4_3113 0.30  +0.2 3,499,730 +  Pf1N1B4_3113 0.34  +0.5 3,499,730 +  Pf1N1B4_3113 0.34  +0.0 3,499,730 +  Pf1N1B4_3113 0.34  +1.4 3,499,730 +  Pf1N1B4_3113 0.34  -0.2 3,499,730 +  Pf1N1B4_3113 0.34  +0.0 3,499,730 +  Pf1N1B4_3113 0.34  -0.1 3,499,730 +  Pf1N1B4_3113 0.34  -0.3 3,499,730 +  Pf1N1B4_3113 0.34  -0.3 3,499,730 +  Pf1N1B4_3113 0.34  +0.9 3,499,730 +  Pf1N1B4_3113 0.34  +1.6 3,499,730 +  Pf1N1B4_3113 0.34  -0.6 3,499,730 +  Pf1N1B4_3113 0.34  +2.4 3,499,730 +  Pf1N1B4_3113 0.34  +0.0 3,499,730 +  Pf1N1B4_3113 0.34  -0.1 3,499,730 +  Pf1N1B4_3113 0.34  +1.0 3,499,730 +  Pf1N1B4_3113 0.34  +0.2 3,499,730 +  Pf1N1B4_3113 0.34  +1.3 3,499,730 +  Pf1N1B4_3113 0.34  -0.2 3,499,730 +  Pf1N1B4_3113 0.34  +0.5 3,499,730 +  Pf1N1B4_3113 0.34  -0.3 3,499,730 +  Pf1N1B4_3113 0.34  +0.8 3,499,730 +  Pf1N1B4_3113 0.34  +0.6 3,499,730 +  Pf1N1B4_3113 0.34  +0.0 3,499,730 +  Pf1N1B4_3113 0.34  -0.7 3,499,730 +  Pf1N1B4_3113 0.34  +1.3 3,499,730 +  Pf1N1B4_3113 0.34  -1.1 3,499,730 +  Pf1N1B4_3113 0.34  -0.7 3,499,730 +  Pf1N1B4_3113 0.34  +0.0 3,499,730 +  Pf1N1B4_3113 0.34  +0.4 3,499,730 +  Pf1N1B4_3113 0.34  -2.0 3,499,730 +  Pf1N1B4_3113 0.34  +0.2 3,499,730 +  Pf1N1B4_3113 0.34  -1.0 3,499,730 +  Pf1N1B4_3113 0.34  -0.6 3,499,730 +  Pf1N1B4_3113 0.34  +0.6 3,499,730 +  Pf1N1B4_3113 0.34  +0.4 3,499,730 +  Pf1N1B4_3113 0.34  -0.4 3,499,730 +  Pf1N1B4_3113 0.34  -0.7 3,499,730 +  -1.3 3,499,730 +  Pf1N1B4_3113 0.34  -0.1 3,499,730 +  Pf1N1B4_3113 0.34  +0.2 3,499,730 +  Pf1N1B4_3113 0.34  +1.3 3,499,730 +  Pf1N1B4_3113 0.34  -2.1 3,499,730 +  Pf1N1B4_3113 0.34  +0.6 3,499,730 +  Pf1N1B4_3113 0.34  -0.8 3,499,730 +  Pf1N1B4_3113 0.34  +0.0 3,499,730 +  Pf1N1B4_3113 0.34  +1.0 3,499,730 +  Pf1N1B4_3113 0.34  +1.4 3,499,730 +  Pf1N1B4_3113 0.34  -0.2 3,499,732 +  Pf1N1B4_3113 0.34  -0.6 3,499,732 +  Pf1N1B4_3113 0.34  -1.0 3,499,732 +  Pf1N1B4_3113 0.34  +1.0 3,499,732 +  Pf1N1B4_3113 0.34  +1.8 3,499,732 +  Pf1N1B4_3113 0.34  +0.6 3,499,732 +  Pf1N1B4_3113 0.34  +0.2 3,499,732 +  Pf1N1B4_3113 0.34  -0.1 3,499,732 +  Pf1N1B4_3113 0.34  +1.6 3,499,732 +  Pf1N1B4_3113 0.34  -0.7 3,499,732 +  -0.6 3,499,732 +  Pf1N1B4_3113 0.34  -0.1 3,499,732 +  Pf1N1B4_3113 0.34  +0.4 3,499,732 +  Pf1N1B4_3113 0.34  +1.3 3,499,732 +  Pf1N1B4_3113 0.34  +0.0 3,499,732 +  Pf1N1B4_3113 0.34  -1.0 3,499,732 +  Pf1N1B4_3113 0.34  -0.8 3,499,732 +  Pf1N1B4_3113 0.34  -1.0 3,499,732 +  Pf1N1B4_3113 0.34  -0.1 3,499,732 +  Pf1N1B4_3113 0.34  +0.2 3,499,732 +  Pf1N1B4_3113 0.34  -0.6 3,499,732 +  Pf1N1B4_3113 0.34  -0.7 3,499,732 +  Pf1N1B4_3113 0.34  +0.3 3,499,732 +  Pf1N1B4_3113 0.34  +1.0 3,499,732 +  Pf1N1B4_3113 0.34  -0.2 3,499,732 +  Pf1N1B4_3113 0.34  +1.7 3,499,732 +  Pf1N1B4_3113 0.34  -1.0 
Or see this region's nucleotide sequence